Argonaute 3 Antibody (JJ201-07)

Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Analysis of paraffin-embedded rat testis tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

Flow Cytometry: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Flow cytometric analysis of N2A cells with Argonaute 3 antibody at 1/50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Staining EIF2C3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Staining EIF2C3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Analysis of paraffin-embedded mouse brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Analysis of paraffin-embedded rat brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (JJ201-07) [NBP2-67851] - Analysis of paraffin-embedded rat spinal cord tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.

Product Discontinued

Argonaute 3 Antibody (JJ201-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human Argonaute 3 aa 689-860/860. (SwissProt: Q9H9G7 Human; SwissProt: Q8CJF9 Mouse; Unigene: 92025 Rat)
• Localization: Cytoplasm,Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGO3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Argonaute 3 Antibody (JJ201-07)

• AGO3argonaute 3
• argonaute3
• eIF2C 3
• eIF-2C 3
• Eukaryotic translation initiation factor 2C 3
• eukaryotic translation initiation factor 2C, 3
• FLJ12765
• hAgo3
• MGC86946
• protein argonaute-3

Background

Argonaute 3 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO3