ARFIP1 Antibody (1F10) - Azide and BSA Free

Western Blot: ARFIP1 Antibody (1F10) [H00027236-M05] - Analysis of ARFIP1 expression in transfected 293T cell line by ARFIP1 monoclonal antibody (M05), clone 1F10. Lane 1: ARFIP1 transfected lysatE (41.7 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: ARFIP1 Antibody (1F10) [H00027236-M05] - Analysis of monoclonal antibody to ARFIP1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml

Sandwich ELISA: ARFIP1 Antibody (1F10) [H00027236-M05] - Detection limit for recombinant GST tagged ARFIP1 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARFIP1 Antibody (1F10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ARFIP1 Antibody (1F10) - Azide and BSA Free (H00027236-M05) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ARFIP1 (NP_055262.1, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQESPKNSAAEIPVTSNGEVDDSREHSFNRDLKHSLPSGLGLSETQITSHGFDNTKEGVIEAGAFQGGQRTQTKSGPVILADEIKNPAMEKLELVRKWSL
• Specificity: ARFIP1 - ADP-ribosylation factor interacting protein 1 (arfaptin 1) (1F10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARFIP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ARFIP1 Antibody (1F10) - Azide and BSA Free

• ADP-ribosylation factor interacting protein 1
• ADP-ribosylation factor-interacting protein 1
• arfaptin 1
• arfaptin-1
• HSU52521
• MGC117369

Background

ADP-ribosylation factors, or ARFs, enhance the ADP ribosyltransferase activity of cholera toxin and are implicated in vesicle transport between endoplasmic reticulum and the Golgi complex. Arfaptin 1 is recruited from the cytosol to Golgi membranes by ARFs in a guanosine 5'-O-(3-thiotriphosphate)-dependent and brefeldin A-sensitive manner but is not a constituent of coatomer. Arfaptin 1 binds to nonmyristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1, another class I ARF. It binds with lower affinity to ARF5, a class II ARF, and with very little affinity to ARF6, a class III ARF. POR1 (also designated arfaptin 2) was first isolated as a Rac 1 binding protein necessary for Rac mediated Actin polymerization and the subsequent formation of membrane ruffles and lamellipodia. POR1 has also been shown to interact with the ADP ribosylation factor ARF6, a GTPase that associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent manner. The association of POR1 with ARF6 stimulates induction of Actin polymerization. POR1 appears to play a regulatory role through multiple signaling pathways in the reorganization of the cytoskeletal structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARFIP1
• Entrez:
  • 27236(Human)
• Uniprot:
  • NP_055262(Human)