ARFGAP3 Antibody - BSA Free

Western Blot: ARFGAP3 Antibody [NBP1-31064] - Sample(30 ug of whole cell lysate)A:293T7. 5% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: ARFGAP3 Antibody [NBP1-31064] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: ARFGAP3 protein stained by ARFGAP3 antibody, C-term diluted at 1:500. Blue: Hoechst 33343 staining.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARFGAP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARFGAP3 Antibody - BSA Free (NBP1-31064) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ARFGAP3. The exact sequence is proprietary.
• Localization: Cytoplasm, Golgi apparatus membrane
• Predicted Species: Mouse (92%), Rat (92%), Chicken (92%), Primate (100%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARFGAP3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (85%), Xenopus Tropicalis (85%), Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ARFGAP3 Antibody - BSA Free

• ADP-ribosylation factor GTPase activating protein 1
• ADP-ribosylation factor GTPase activating protein 3
• ADP-ribosylation factor GTPase-activating protein 3
• ARF GAP 3
• ARFGAP1
• FLJ45618

Background

The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ARFGAP3