ARF6 Antibody (JF101-2)

Western Blot: ARF6 Antibody (JF101-2) [NBP2-67165] - Analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ARF6 Antibody (JF101-2) [NBP2-67165] - Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-ARF6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

ARF6 Antibody (JF101-2) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ARF6 aa 133-165 / 175. (SwissProt: P62330 Human; SwissProt: P62331 Mouse; SwissProt: P62332 Rat)
• Localization: Golgi apparatus, Cell membrane, Endosome membrane, Cell projection, Midbody, Cytoplasm, Cleavage furrow.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARF6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ARF6 Antibody (JF101-2)

• ADP-ribosylation factor 6
• ARF6
• DKFZp564M0264
• EC 3.6.5.2

Background

The ARF6 gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encodesmall guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and playa role in vesicular tr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ARF6 Antibody (NBP2-67165). (Showing 1 - 2 of 2 FAQ).

1. We would like to buy a myristoylated Arf6 purified protein. If you have myristoylated Arf6 purified protein, please let me know.
   • Unfortunately, due to our expression system as well as Abnova's, we do not believe that these proteins have post-translational modifications similar to the endogenous protein
2. I saw another protein purified product ARF6, which is expressed in E.coli. Did you expressed that protein with n-myristoyltransferase co-transformation E.coli?
   • We do not express the ARF6 protein with n-myristoyltransferase co-transformation E.coli.

Bioinformatics

• Gene Symbol: ARF6