ARF1,2,3,4 Antibody

Images

 
Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of A431 (A) and (0.3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: ARF1,2,3,4 Antibody [NB100-55421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of NIH3T3 (A), KNRK (B) and MDCK (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ARF1,2,3,4 Antibody Summary

Immunogen
Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1.
Epitope
EGLDWLSNQLRNQK
Specificity
This antibody is expected to recognize all four reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 18 kDa band observed in HeLa, A431, MCF7, NIH3T3, KNRK, and MDCK lysates (calculated MW of 20.7 kDa band according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1).
Control
PACAP/ADCYAP1 Overexpression Lysate
Publications
Read Publications using NB100-55421.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARF1,2,3,4 Antibody

  • ADP-ribosylation factor 1

Background

DP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARF1,2,3,4 Antibody (NB100-55421)(2)

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARF1
Entrez
Uniprot