ARF1,2,3,4 Antibody

Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of A431 (A) and (0.3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Flow Cytometry: ARF1,2,3,4 Antibody [NB100-55421] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: ARF1,2,3,4 Antibody [NB100-55421] - Staining (1ug/ml) of NIH3T3 (A), KNRK (B) and MDCK (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, Flow, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARF1,2,3,4 Antibody Summary

• Immunogen: Peptide with sequence C-EGLDWLSNQLRNQK, from the C Terminus of the protein sequence according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1.
• Epitope: EGLDWLSNQLRNQK
• Specificity: This antibody is expected to recognize all four reported isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 18 kDa band observed in HeLa, A431, MCF7, NIH3T3, KNRK, and MDCK lysates (calculated MW of 20.7 kDa band according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1).
• Control:

  ARF1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARF1,2,3,4 Antibody

• ADP-ribosylation factor 1

Background

DP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARF1,2,3,4 Antibody (NB100-55421)(2)

Publications using NB100-55421

• Klein S, Partisani M, Franco M et al. EFA6 Facilitates the Assembly of the Tight Junction by Coordinating an Arf6-dependent -independent Pathway. J Biol Chem;283(44):30129-30138. 2008-01-01 [PMID: 18779331]
• Dubois T, Paleotti O, Mironov AA et al. Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics. Nat Cell Biol 2005-04-01 [PMID: 15793564]

Bioinformatics

• Gene Symbol: ARF1
• Entrez:
  • 375(Human)
  • 11840(Mouse)
  • 64310(Rat)
• Uniprot:
  • NP_001019397.1(Human)