ARF1 Antibody (1D9)

Western Blot: ARF1 Antibody (1D9) [NB300-505] - Analysis of canine heart extract.

Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - Analysis of ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.

Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of MCF-7 cells compared to an isotype control (blue).

Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - Analysis of ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human liver tissues.

Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of 3T3 cells compared to an isotype control (blue).

Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of Hela cells compared to an isotype control (blue).

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
• Clone: 1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ARF1 Antibody (1D9) Summary

• Immunogen: Recombinant human ARF1.
• Localization: Golgi Apparatus
• Specificity: Detects ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARF1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:20
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: ELISA usage was reported in scientific literature. WB: Detects an approx. 21 kDa protein representing Arf in rat pancreas extract. IF: Staining of Arf in rat pancreas results in staining of the cytoplasmic face of the trans-Golgi membranes.This antibody cannot be used to neutralize Arf in solution.
• Control:

  PACAP/ADCYAP1 Overexpression Lysate
  ARF1 Knockout HeLa Cell Lysate

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 10747089). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for ARF1 Antibody (1D9)

• ADP-ribosylation factor 1

Background

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARF1 Antibody (NB300-505)(13)

Publications using NB300-505

• Wei M, Zhu Z, Wu J et al. DRAM1 deficiency affects the organization and function of the Golgi apparatus Cell. Signal. Jul 26 2019 [PMID: 31356858] (WB, Human)
• Nawrotek A, Benabdi S et al. PH-domain-binding inhibitors of nucleotide exchange factor BRAG2 disrupt Arf GTPase signaling. Nat Chem Biol Jan 4 2019 [PMID: 30742123] (ICC/IF, Human)
• Ramirez-Peinado S, Ignashkova TI, van Raam BJ et al. TRAPPC13 modulates autophagy and the response to Golgi stress. J. Cell. Sci. May 23 2017 [PMID: 28536105]
• Reiling JH, Olive AJ, Sanyal S et al. A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens. Nat Cell Biol. 2013 Dec [PMID: 24185178] (WB, Human)
• Wang J, Sun HQ, Macia E, Kirchhausen T, Watson H, Bonifacino JS, Yin HL. PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal. Mol Biol Cell;18(7):2646-55. 2007 Jul. [PMID: 17494868] (ICC/IF)
• Shen X, Xu KF, Fan Q, Pacheco-Rodriguez G, Moss J, Vaughan M. Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake. Proc Natl Acad Sci U S A;103(8):2635-40. 2006 Feb 21. [PMID: 16477018] (ELISA)
• Belov GA, Fogg MH, Ehrenfeld E. Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor. J Virol;79(11):7207-16. 2005 Jun. [PMID: 15890959] (WB)
• Chan D, Strang M, Judson B, Brown WJ. Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes. Mol Biol Cell. 15(4):1871-80. 2004 Apr. [PMID: 14767064] (ICC/IF)
• Spitzenberger F, Pietropaolo S, Verkade P, Habermann B, Lacas-Gervais S, Mziaut H, Pietropaolo M, Solimena M. Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells. J Biol Chem;278(28):26166-73. 2003 Jul 11. [PMID: 12682071] (IP)
• Lu L, Horstmann H, Ng C, Hong W. Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J Cell Sci;114(Pt 24):4543-55. 2001 Dec. [PMID: 11792819] (ICC/IF)
• Cavenagh MM, Whitney JA, Carroll K, Zhang C, Boman AL, Rosenwald AG, Mellman I, Kahn RA. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J Biol Chem;271(36):21767-74. 1996 Sep 6. [PMID: 8702973] (ELISA)
• Serafini T, Orci L, Amherdt M, Brunner M, Kahn RA, Rothman JE. ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein. Cell;67(2):239-53. 1991 Oct 18. [PMID: 1680566] (WB)
• Zeuzem S, Feick P, Zimmermann P, Haase W, Kahn RA, Schulz I. Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes. Proc Natl Acad Sci U S A;89(14):6619-23. 1992 Jul 15. [PMID: 1385876] (IHC)

Bioinformatics

• Gene Symbol: ARF1
• Entrez:
  • 375(Human)
  • 11840(Mouse)
  • 64310(Rat)
• Uniprot:
  • P84077(Human)