ELISA usage was reported in scientific literature. WB: Detects an approx. 21 kDa protein representing Arf in rat pancreas extract. IF: Staining of Arf in rat pancreas results in staining of the cytoplasmic face of the trans-Golgi membranes.This antibody cannot be used to neutralize Arf in solution.
Bovine reactivity reported in scientific literature (PMID: 10747089). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Ascites diluted with PBS
0.05% Sodium Azide
Alternate Names for ARF1 Antibody (1D9)
ADP-ribosylation factor 1
The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Arf1: A New Focus In Cancer Drug Therapy ARF1 (ADP-ribosylation factor 1) is a protein in the ARF gene family that is responsible for vesicular trafficking within the cell through its activation of phospholipase D. It is found in the cells golgi apparatus and its main function is intra-Golgi... Read full blog post.
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