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ARF1 Antibody (1D9)

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Western Blot: ARF1 Antibody (1D9) [NB300-505] - Analysis of canine heart extract.
Western Blot: ARF1 Antibody (1D9) [NB300-505] - Analysis of canine heart extract.
Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - Analysis of ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of MCF-7 cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunocytochemistry/ Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - Analysis of ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human liver tissues.
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of 3T3 cells compared to an isotype control (blue).
Flow Cytometry: ARF1 Antibody (1D9) [NB300-505] - Analysis of Hela cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clone
1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ARF1 Antibody (1D9) Summary

Immunogen
Recombinant human ARF1.
Localization
Golgi Apparatus
Specificity
Detects ADP-ribosylation factor1 (Arf 1), Arf 3, Arf 5 and Arf 6 and at about a ten-fold reduction with Arf 4.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ARF1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:20
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500
Application Notes
ELISA usage was reported in scientific literature. WB: Detects an approx. 21 kDa protein representing Arf in rat pancreas extract. IF: Staining of Arf in rat pancreas results in staining of the cytoplasmic face of the trans-Golgi membranes.This antibody cannot be used to neutralize Arf in solution.
Control
ARF1 Overexpression Lysate
Publications
Read Publications using
NB300-505 in the following applications:

  • ELISA
    2 publications
  • ICC/IF
    4 publications
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 10747089). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for ARF1 Antibody (1D9)

  • ADP-ribosylation factor 1
  • ARF1

Background

The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. The Arf family is divided functionally into the Arf and the Arf-like (Arl) proteins. Arfs share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: Cytohesin 2 Antibody (6H5) [H00009266-M02] - Analysis of monoclonal antibody to CYTH2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Cytohesin 2 Antibody (6H5) [H00009266-M02] - Analysis of monoclonal antibody to CYTH2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
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Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP1-85727] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP1-85727] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus. Antibody staining is shown in green.
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Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Species: Hu, Mu
Applications: ICC/IF, WB
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
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Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.
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(1 Publication)
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Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human tonsil tissue.
Immunohistochemistry-Paraffin: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human breast carcinoma.
Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human placenta tissue.
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Immunohistochemistry-Paraffin: ARF3 Antibody [NBP2-99493] - Immunochemical staining ARF3 in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: ARF3 Antibody [NBP2-99493] - Immunochemical staining ARF3 in human kidney with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.
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Western Blot: ARF4 Antibody [NBP2-26147] - NIH3T3 (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ARF4 Antibody [NBP2-26147] - Human Thymus tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Immunocytochemistry/Immunofluorescence: ARFGAP3 Antibody [NBP1-88842] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ARFGAP3 Antibody [NBP1-88842] - Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.
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Western Blot: ARF5 Antibody (1B4) [H00000381-M01] - Analysis of ARF5 expression in transfected 293T cell line by ARF5 monoclonal antibody (M01), clone 1B4.Lane 1: ARF5 transfected lysate(20.5 KDa).Lane 2: Non-transfected lysate.
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Flow Cytometry: ARFGAP1 Antibody (1F6) [NBP2-00513] - Analysis of Hela cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: ARFGAP1 Antibody (1F6) [NBP2-00513] - Analysis of Jurkat cells, using anti-ARFGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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Immunohistochemistry-Paraffin: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of paraffin-embedded mouse brain tissue using anti-FosB antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of paraffin-embedded rat spleen tissue using anti-FosB antibody. Counter stained with hematoxylin.
Western Blot: FosB/G0S3 Antibody (SN703) [NBP2-67710] - Analysis of FosB on human liver lysates using anti-FosB antibody at 1/1,000 dilution.
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Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ARF1 Antibody (1D9)
NB300-505
ARF1 Antibody (1D9)
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP

Publications for ARF1 Antibody (NB300-505)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA, ICC/IF, IHC, IP, WB.


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ELISA
(2)
ICC/IF
(4)
IHC
(1)
IP
(1)
WB
(4)
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Human
(3)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB300-505 Applications Species
Wei M, Zhu Z, Wu J et al. DRAM1 deficiency affects the organization and function of the Golgi apparatus Cell. Signal. Jul 26 2019 [PMID: 31356858] (WB, Human) WB Human
Nawrotek A, Benabdi S et al. PH-domain-binding inhibitors of nucleotide exchange factor BRAG2 disrupt Arf GTPase signaling. Nat Chem Biol Jan 4 2019 [PMID: 30742123] (ICC/IF, Human) ICC/IF Human
Ramirez-Peinado S, Ignashkova TI, van Raam BJ et al. TRAPPC13 modulates autophagy and the response to Golgi stress. J. Cell. Sci. May 23 2017 [PMID: 28536105]
Reiling JH, Olive AJ, Sanyal S et al. A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens. Nat Cell Biol. 2013 Dec [PMID: 24185178] (WB, Human) WB Human
Wang J, Sun HQ, Macia E, Kirchhausen T, Watson H, Bonifacino JS, Yin HL. PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal. Mol Biol Cell;18(7):2646-55. 2007 Jul. [PMID: 17494868] (ICC/IF) ICC/IF
Shen X, Xu KF, Fan Q, Pacheco-Rodriguez G, Moss J, Vaughan M. Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake. Proc Natl Acad Sci U S A;103(8):2635-40. 2006 Feb 21. [PMID: 16477018] (ELISA) ELISA
Belov GA, Fogg MH, Ehrenfeld E. Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor. J Virol;79(11):7207-16. 2005 Jun. [PMID: 15890959] (WB) WB
Chan D, Strang M, Judson B, Brown WJ. Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes. Mol Biol Cell. 15(4):1871-80. 2004 Apr. [PMID: 14767064] (ICC/IF) ICC/IF
Spitzenberger F, Pietropaolo S, Verkade P, Habermann B, Lacas-Gervais S, Mziaut H, Pietropaolo M, Solimena M. Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells. J Biol Chem;278(28):26166-73. 2003 Jul 11. [PMID: 12682071] (IP) IP
Lu L, Horstmann H, Ng C, Hong W. Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J Cell Sci;114(Pt 24):4543-55. 2001 Dec. [PMID: 11792819] (ICC/IF) ICC/IF
Show All 13 Publications.

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Product General Protocols

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WB Video Protocol
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FAQs for ARF1 Antibody (NB300-505) (0)

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Control Lysate(s)

ARF1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Arf1: A New Focus In Cancer Drug Therapy
ARF1 (ADP-ribosylation factor 1) is a protein in the ARF gene family that is responsible for vesicular trafficking within the cell through its activation of phospholipase D. It is found in the cells golgi apparatus and its main function is intra-Golgi...  Read full blog post.
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Bioinformatics

Gene Symbol ARF1
Entrez
Uniprot