Apolipoprotein B/ApoB Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Apolipoprotein B/ApoB Antibody Summary

Immunogen
Apolipoprotein purified from human plasma.
Specificity
It shows negligible cross-reactivity with type A-I, A-II, C-I, C-II, C-III, E, and J apoLipoproteins (<1%).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APOB
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blotting (0.5-1 ug/ml), immunoprecipitation (5-10 ug/ml), Immunohistochemistry (5-10 ug/ml) and Elisa (0.5-1 ug/ml). However, the optimal conditions should be determined individually. The antibody reacts with human apoLipoprotein B. It shows negligible cross-reactivity with type A-I, A-II, C-I, C-II, C-III, E, and J apoLipoproteins (<1%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein B/ApoB Antibody

  • Apo B-100
  • APOB
  • apoB-100
  • apoB-48
  • apolipoprotein B (including Ag(x) antigen)
  • Apolipoprotein B
  • apolipoprotein B-100
  • apolipoprotein B48
  • FLDB
  • LDLCQ4
  • mutant Apo B 100

Background

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol APOB
Entrez
Uniprot