Apolipoprotein B/ApoB Antibody

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878] - Staining of paraffin embedded Human Small Intestine with antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Apolipoprotein B/ApoB Antibody [NBP1-20878] - Staining of paraffin embedded Human Liver with antibody at 2 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein B/ApoB Antibody Summary

• Description: Novus Biologicals Goat Apolipoprotein B/ApoB Antibody (NBP1-20878) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Apolipoprotein B/ApoB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This Apolipoprotein B/ApoB Antibody was developed against a peptide with sequence C-TDLHLRYQKDKK corresponding to internal region according to NP_000375.2.
• Epitope: C terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 ug/mL
  • Immunohistochemistry-Paraffin 2 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein B/ApoB Antibody

• Apo B-100
• APOB
• ApoB-100
• ApoB-48
• apolipoprotein B (including Ag(x) antigen)
• Apolipoprotein B
• apolipoprotein B-100
• apolipoprotein B48
• FLDB
• LDLCQ4
• mutant Apo B 100

Background

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein B/ApoB Antibody (NBP1-20878)(1)

Publication using NBP1-20878

• Benn M, Nordestgaard BG, Jensen JS, Tybjaerg-Hansen A. Polymorphisms in apolipoprotein B and risk of ischemic stroke. J Clin Endocrinol Metab 2007-09-01 [PMID: 17595251]

Bioinformatics

• Gene Symbol: APOB
• Entrez:
  • 338(Human)
• Uniprot:
  • NP_000375.2(Human)