Apc5 Overexpression Lysate

Images

 
Western Blot: Apc5 Overexpression Lysate (Adult Normal) [NBP2-04352] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Apc5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Apc5 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_057321. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Apc5, mRNA.
Gene
ANAPC5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
84.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Apc5 Overexpression Lysate

  • anaphase promoting complex subunit 5
  • anaphase-promoting complex subunit 5
  • APC5Cyclosome subunit 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-91662
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-66987
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89095
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-61889
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-77375
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89093
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-57669
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-01128
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF4497
Species: Hu
Applications: WB, Simple Western
NB100-59824
Species: Hu, Mu
Applications: WB, IP
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-20389
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67820
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP2-61656
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-13286
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-40367
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-31361
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-04352
Species: Hu
Applications: WB

Publications for Apc5 Lysate (NBP2-04352) (0)

There are no publications for Apc5 Lysate (NBP2-04352).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Apc5 Lysate (NBP2-04352) (0)

There are no reviews for Apc5 Lysate (NBP2-04352). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Apc5 Lysate (NBP2-04352) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Apc5 Products

Bioinformatics Tool for Apc5 Lysate (NBP2-04352)

Discover related pathways, diseases and genes to Apc5 Lysate (NBP2-04352). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Apc5 Lysate (NBP2-04352)

Discover more about diseases related to Apc5 Lysate (NBP2-04352).
 

Pathways for Apc5 Lysate (NBP2-04352)

View related products by pathway.

Research Areas for Apc5 Lysate (NBP2-04352)

Find related products by research area.

Blogs on Apc5

There are no specific blogs for Apc5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Apc5 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ANAPC5