Apc10 Antibody

Western Blot: Apc10 Antibody [H00010393-D01P] - Analysis of ANAPC10 expression in transfected 293T cell line by ANAPC10 polyclonal antibody.Lane 1: ANAPC10 transfected lysate(21.30 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Apc10 Antibody Summary

• Immunogen: ANAPC10 (AAH05217.1, 1 a.a. - 185 a.a.) full-length human protein. MTTPNKTPPGADPKQLERTGTVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGSQPHLVNIQFRRKTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWIHVPLTDNHKKPTRTFMIQIAVLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMMYRSIR
• Specificity: ANAPC10 - anaphase promoting complex subunit 10,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Apc10 Antibody

• anaphase promoting complex subunit 10
• anaphase-promoting complex subunit 10
• APC10DOC1
• Cyclosome subunit 10
• DKFZP564L0562

Background

APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANAPC10
• Entrez:
  • 10393(Human)
• Uniprot:
  • AAH05217.1(Human)