AP2S1 Antibody (JE47-37)

Western Blot: AP2S1 Antibody (JE47-37) [NBP3-32041] - Western blot analysis of AP2S1 on different lysates with Rabbit anti-AP2S1 antibody (NBP3-32041) at 1/1,000 dilution. Lane 1: K-562 cell lysate (30 ug/Lane) Lane 2: MCF7 cell lysate (30 ug/Lane) Lane 3: NIH/3T3 cell lysate (30 ug/Lane) Lane 4: PC-12 cell lysate (30 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Lane 6: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 14 kDa Exposure time: 1 minute 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32041) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE47-37
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AP2S1 Antibody (JE47-37) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human AP2S1 aa 1-50 / 142. (Uniprot: P53680)
• Localization: Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AP2S1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AP2S1 Antibody (JE47-37)

• Adapter-related protein complex 2 sigma subunit
• Adaptor protein complex AP-2 subunit sigma
• adaptor-related protein complex 2, sigma 1 subunit
• AP17HA2 17 kDa subunit
• AP-2 complex subunit sigma
• CLAPS2AP17-DELTA
• Clathrin assembly protein 2 small chain
• Clathrin coat assembly protein AP17
• Clathrin coat-associated protein AP17
• clathrin-associated/assembly/adaptor protein, small 2 (17kD)
• Plasma membrane adaptor AP-2 17 kDa protein
• sigma2-adaptin

Background

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP2S1