ANKRD15 Overexpression Lysate

Images

 
Western Blot: ANKRD15 Overexpression Lysate (Adult Normal) [NBP2-10297] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ANKRD15.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ANKRD15 Overexpression Lysate Summary

Description

ANKRD15 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC207331

Accession#: NM_015158

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
KANK1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
147.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ANKRD15 Overexpression Lysate

  • ANKRD15ankyrin repeat domain 15
  • Ankyrin repeat domain-containing protein 15
  • KANKDKFZp451G231
  • KIAA0172MGC43128
  • Kidney ankyrin repeat-containing protein
  • KN motif and ankyrin repeat domain-containing protein 1
  • KN motif and ankyrin repeat domains 1

Background

The ANKRD15 gene encodes a protein containing four ankyrin repeat domains in its C-terminus. The suggested role for this protein is in tumorigenesis of renal cell carcinoma. Two alternatively spliced transcript variants encoding different isoforms have been iden

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr,  IHC-P, WB
NBP2-59451
Species: Hu
Applications: ELISA, Flow, ICC/IF, MiAr, Simple Western, WB
NBP2-46471
Species: Hu, Mu, Rt
Applications: IHC,  IHC-P, WB
NBP1-84071
Species: Hu
Applications: IHC,  IHC-P, WB
NBP1-88711
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NBP1-33736
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, IP, WB
NBP2-37969
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
NBP1-80963
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
AF1042
Species: Mu
Applications: Dual ISH-IHC, IHC, WB
AF2408
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, KO, Simple Western, WB
NBP2-34031
Species: Hu
Applications: ICC/IF, IHC,  IHC-P, WB
NBP3-46466
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, WB
NBP2-31844
Species: Hu
Applications: ICC/IF, IHC,  IHC-P
NBP1-92025
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NBP3-15868
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC,  IHC-P, WB
NBP2-69049
Species: Hu
Applications: IHC,  IHC-P
NB400-141
Species: Ha, Hu, Mu
Applications: ICC/IF, IP, WB
H00002298-P01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
AF3505
Species: Hu
Applications: CyTOF-ready, Flow, WB
NBP2-10297
Species: Hu
Applications: WB

Publications for ANKRD15 Lysate (NBP2-10297) (0)

There are no publications for ANKRD15 Lysate (NBP2-10297).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ANKRD15 Lysate (NBP2-10297) (0)

There are no reviews for ANKRD15 Lysate (NBP2-10297). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ANKRD15 Lysate (NBP2-10297) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ANKRD15 Products

Research Areas for ANKRD15 Lysate (NBP2-10297)

Find related products by research area.

Blogs on ANKRD15

There are no specific blogs for ANKRD15, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ANKRD15 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol KANK1