Angiostatin Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Host: Goat
• Conjugate: Alexa Fluor 700

Angiostatin Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Angiostatin Kringle 1-3
  Val98-Ser358 with Cys188Ser & Cys316Ser substitutions
  Accession # P00747
• Specificity: Detects human Angiostatin in direct ELISAs and Western blots. This antibody will also recognize human plasminogen.
• Isotype: IgG
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiostatin Antibody [Alexa Fluor® 700]

• Angiostatin
• HAE4

Background

Angiostatin is a 38-45 kDa proteolytic fragment of plasminogen, a 92-100 kDa glycosylated blood zymogen that serves as the precursor for plasmin.  Mature human plasminogen is 713 amino acids (aa) in length (aa 98-810).  It contains five N-terminal kringle domains (aa 103-561) plus one peptidase S1 domain (aa 581-808). Upon cleavage by tPA between Arg580 and Val581, plasminogen is converted into the disulfide-linked two-subunit enzyme plasmin that dissolves fibrin clots.  Plasminogen is also cleaved by MMPs which generate multiple fragments.  Angiostatin has documented antiangiogenic and antitumor activity, likely mediated by a downregulation of Bcl2, and an upregulation of thrombospondin-1.  Over aa 98-358, human angiostatin shares 81% aa identity with mouse angiostatin.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.