Recombinant Human AMELX His Protein

Recombinant Human AMELX Protein [NBP2-22683] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human AMELX His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 17-191 of Human AMELX
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPLPPHP GHPGYINFSY EVLTPLKWYQ SIRPPYPSYG YEPMGGWLHH QIIPVLSQQH PPTHTLQPHH HIPVVPAQQP VIPQQPMMPV PGQHSMTPIQ HHQPNLPPPA QQPYQPQPVQ PQPHQPMQPQ PPVHPMQPLP PQPPLPPMFP MQPLPPMLPD LTLEAWPSTD KTKREEVD

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AMELX
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.5), 0.2 M NaCl, 30% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human AMELX His Protein

• AIH1
• ALGN
• amelogenin (amelogenesis imperfecta 1, X-linked)
• amelogenin (X chromosome, amelogenesis imperfecta 1)
• amelogenin, X isoform
• amelogenin, X-linked
• AMGAMGL
• AMGXamelogenesis imperfecta 1

Background

AMELX is a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in AMELX cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. Recombinant human AMELX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for AMELX Recombinant Protein (NBP2-22683)(1)

Publication using NBP2-22683

• Petronijevic S, Stig S, Halstensen T Epitope mapping of anti-amelogenin IgA in coeliac disease Eur. J. Oral Sci. 2020-02-01 [PMID: 32084301] (Cytometric Bead Assay Standard, Human)

Bioinformatics

• Gene Symbol: AMELX