AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

Western Blot: AlphaB Crystallin/CRYAB [p Ser45] Antibody [NB120-5598] - Analysis of recombinant bovine phospho-alpha-B crystallin (Ser45). Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.

Product Discontinued

AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free Summary

• Immunogen: Synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.
• Modification: p Ser45
• Localization: Cytoplasmic
• Specificity: This does not detect the unphosphorylated form of the protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRYAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 8 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: WB: Detects an approx. 20 kDa representing recombinant bovine phospho-alpha-B crystallin (Ser45). IF: Staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.
• Control:

  AlphaB Crystallin/CRYAB Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute to desired concentration.

Alternate Names for AlphaB Crystallin/CRYAB [p Ser45] Antibody - BSA Free

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products--alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease where it accumulates in brain cells of those afflicted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NB120-5598)(1)

Publication using NB120-5598

• Ueda S, Nishihara M, Hioka Y et al. Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5 International Journal of Molecular Sciences 2022-09-24 [PMID: 36232565] (Western Blot, Immunocytochemistry/ Immunofluorescence)

Bioinformatics

• Gene Symbol: CRYAB
• Entrez:
  • 1410(Human)
  • 12955(Mouse)
  • 25420(Rat)