AlphaB Crystallin/CRYAB [p Ser19] Antibody

Product Discontinued

AlphaB Crystallin/CRYAB [p Ser19] Antibody Summary

• Immunogen: Synthetic peptide: FFPFHSPSRLFD, corresponding to amino acids 14-25 of Human Crystallin (alpha B).
• Modification: p Ser19
• Localization: Cytoplasmic
• Specificity: NB120-5597 detects phospho-alpha-B crystallin (Ser19) from bovine, human, mouse and rat samples. This does not detect the unphosphorylated form of the protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRYAB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Western Blot
• Application Notes: WB: Detects a band of approximately 20 kDa representing phospho-alpha-B crystallin (Ser19) from human cell extract.Immunofluorescence staining of phospho-alpha-B crystallin (Ser19) in U373 MG cells results in cytoplasmic staining. Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  AlphaB Crystallin/CRYAB Overexpression Lysate

Reactivity Notes

Cross-reacts with Cow, Human, Mouse and Rat.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 3% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AlphaB Crystallin/CRYAB [p Ser19] Antibody

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AlphaB Crystallin/CRYAB Antibody (NB120-5597)(1)

Publication using NB120-5597

• Dong Y, Dong Z, Kase S et al. Phosphorylation of alphaB-crystallin in epiretinal membrane of human proliferative diabetic retinopathy. Int J Ophthalmol. 2016-09-02 [PMID: 27588262] (Human)

Bioinformatics

• Gene Symbol: CRYAB
• Entrez:
  • 1410(Human)
  • 12955(Mouse)
  • 25420(Rat)