alpha-2A Adrenergic R/ADRA2A Antibody

Product Discontinued

alpha-2A Adrenergic R/ADRA2A Antibody Summary

• Immunogen: A synthetic peptide (FGYCNSSLNPVIYTIFNH) corresponding to amino acids 414-431 from human Adrenergic Receptor alpha 2a. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
• Specificity: The specificity of this antibody has been confirmed by WB and IHC against the antigen.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADRA2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2.0 ug/ml
  • Western Blot 1.0 ug/ml
• Application Notes: This antibody is useful in Western Blot Human Adrenergic Receptor alpha 2a has a predicted length of 450 amino acids and MW of 49 kDa. This antibody may also be useful in Immunohistochemistry-paraffin.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 mg DiSodium Phosphate, 0.9 mg NaCl and 5 mg BSA
• Preservative: 0.05mg Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.1 ml sterilized distilled water to a final concentration of 1.0 mg/ml. Centrifuge to remove any insoluble material.

Alternate Names for alpha-2A Adrenergic R/ADRA2A Antibody

• a-2A AdrenergicR
• ADRA2
• ADRA2A
• ADRA2Ralpha-2-adrenergic receptor, platelet type
• ADRARZNF32
• adrenergic, alpha-2A-, receptor
• Alpha-2 adrenergic receptor subtype C10
• alpha-2A Adrenergic R
• alpha-2A adrenergic receptor
• alpha2A AdrenergicR
• Alpha-2A adrenoceptor
• Alpha-2A adrenoreceptor
• alpha-2AAR subtype C10
• ALPHA2AAR
• Alpha-2AAR
• ZNF32

Background

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq]. Sequence Note: The 5'-most in-frame translation initiation codon is selected for this RefSeq based on good conservation across mammalian species. A possible downstream start codon would result in a protein that is 15 aa shorter at the N-terminus. The literature, including PMIDs:2823383, 2568356, 1354394 and 1678390, assumes the use of the downstream start codon based on initial cloning reports, but there is no experimental evidence indicating which start codon is preferentially used in vivo. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available transcript and conservation data. Two possible start codons are present in this update, with each having a weak Kozak signal. Due to leaky scanning by ribosomes, it is possible that some ribosomes may initiate translation from the upstream AUG while others start from the downstream AUG. Translation initiation from the downstream AUG would result in a protein that is 15 aa shorter at the N-terminus. The literature, including PMIDs 2823383, 2568356, 1354394 and 1678390, assumes the use of the downstream start codon based on initial cloning reports, but there is no experimental evidence indicating which start codon is preferentially used in vivo.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-2A Adrenergic R/ADRA2A Antibody (R-1034-100)(1)

Publication using R-1034-100

• Yang J, Dolinger M, Ritaccio G et al. Leucine Stimulates Insulin Secretion via Down-regulation of Surface Expression of Adrenergic alpha 2A Receptor through the mTOR (Mammalian Target of Rapamycin) Pathway: IMPLICATION IN NEW-ONSET DIABETES IN RENAL TRANSPLANTATION J Biol Chem 2012-07-13 [PMID: 22645144] (WB, Human)

Bioinformatics

• Gene Symbol: ADRA2A
• Entrez:
  • 150(Human)
  • 11551(Mouse)
  • 25083(Rat)
• Uniprot:
  • P08913(Human)