alpha-2A Adrenergic R/ADRA2A Antibody

Product Discontinued

alpha-2A Adrenergic R/ADRA2A Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor alpha-2A. The immunogen region is: C-terminal.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADRA2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for alpha-2A Adrenergic R/ADRA2A Antibody

• a-2A AdrenergicR
• ADRA2
• ADRA2A
• ADRA2Ralpha-2-adrenergic receptor, platelet type
• ADRARZNF32
• adrenergic, alpha-2A-, receptor
• Alpha-2 adrenergic receptor subtype C10
• alpha-2A Adrenergic R
• alpha-2A adrenergic receptor
• alpha2A AdrenergicR
• Alpha-2A adrenoceptor
• Alpha-2A adrenoreceptor
• alpha-2AAR subtype C10
• ALPHA2AAR
• Alpha-2AAR
• ZNF32

Background

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq]. Sequence Note: The 5'-most in-frame translation initiation codon is selected for this RefSeq based on good conservation across mammalian species. A possible downstream start codon would result in a protein that is 15 aa shorter at the N-terminus. The literature, including PMIDs:2823383, 2568356, 1354394 and 1678390, assumes the use of the downstream start codon based on initial cloning reports, but there is no experimental evidence indicating which start codon is preferentially used in vivo. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available transcript and conservation data. Two possible start codons are present in this update, with each having a weak Kozak signal. Due to leaky scanning by ribosomes, it is possible that some ribosomes may initiate translation from the upstream AUG while others start from the downstream AUG. Translation initiation from the downstream AUG would result in a protein that is 15 aa shorter at the N-terminus. The literature, including PMIDs 2823383, 2568356, 1354394 and 1678390, assumes the use of the downstream start codon based on initial cloning reports, but there is no experimental evidence indicating which start codon is preferentially used in vivo.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha-2A Adrenergic R/ADRA2A Antibody (NBP1-67832)(2)

Publications using NBP1-67832

• Alhamyani A, Mahmood ASMH, Alshamrani A et al. Central Type II Glucocorticoid Receptor Regulation of Ventromedial Hypothalamic Nucleus Glycogen Metabolic Enzyme and Glucoregulatory Neurotransmitter Marker Protein Expression in the Male Rat J Endocrinol Diabetes 2021-01-13 [PMID: 34258390] (Western Blot)
• Hasan Mahmood ASM, Mandal SK, Bheemanapally K et al. Norepinephrine control of ventromedial hypothalamic nucleus glucoregulatory neurotransmitter expression in the female rat: Role of monocarboxylate transporter function. Mol. Cell. Neurosci. 2019-01-17 [PMID: 30660767] (WB, Rat)

FAQs for alpha-2A Adrenergic R/ADRA2A Antibody (NBP1-67832). (Showing 1 - 2 of 2 FAQs).

1. Can you please specify whether blocking peptides are available for the following antibodies? NBP1-19562 and NBP1-67832
   • Unfortunately we do not have any blocking peptides available for these antibodies at this time. If we had them available they would be under the same catalog number with a PEP at the end of it.
2. Do you have any reference regarding the use of the antibodies NBP1-19562 and NBP1-67832 on rat brain?
   • At this time we do not have any product specific references available, but will guarantee the use in rat as it is indicated on the datasheet. If the protein is expressed in the customer's rat samples then they can receive a full refund or replacement should it not work as specified.

Bioinformatics

• Gene Symbol: ADRA2A
• Entrez:
  • 150(Human)
• OMIM:
  • 104210(Human)
• Uniprot:
  • P08913(Human)