Recombinant Human ALKBH5 GST (N-Term) Protein


SDS-Page: Recombinant Human ALKBH5 Protein [H00054890-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, PA, AP

Order Details

Recombinant Human ALKBH5 GST (N-Term) Protein Summary

A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-458 of Human ALKBH5 full-length ORF

Source: Wheat Germ (in vitro)


in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Wheat germ
Protein/Peptide Type
Full Length Recombinant Protein
>80% by SDS-PAGE and Coomassie blue staining


  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
77.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
No Preservative
>80% by SDS-PAGE and Coomassie blue staining


This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human ALKBH5 GST (N-Term) Protein

  • ABH5
  • alkB, alkylation repair homolog 5 (E. coli)
  • ALKBH5
  • Alkylated DNA repair protein alkB homolog 5
  • EC 1.14.11.-
  • EC
  • FLJ20308
  • OFOXD1
  • oxoglutarate and iron-dependent oxygenase domain containing
  • probable alpha-ketoglutarate-dependent dioxygenase ABH5


ALKBH5, RNA demethylase ALKBH5, ALKB homolog 5 (44 kDa) is a dioxygenase enzyme which demethylates RNA in a reaction requiring oxygen, alpha-ketoglutarate, and iron. ALKBH5 and fat mass and obesity-associated protein (FTO) are involved in the demethylation of N6-methyladenosine or m6A mRNA, the most common post-translational modification in eukaryotic mRNA. m6A mRNA modifications are very dynamic; they are introduced into the mRNA by m6A methyltransferases (e.g., METTL3/14 and KIAA1429), removed by erasers such as FTO and ALKBH5, and decoded by m6A binding proteins (e.g., IGF2BP1 and YTHDF1/2/3). m6A mRNA modifications play roles in regulating mRNA splicing, processing, translation and stability. m6A mRNA modifications are also associated with pathological states including cancer (1, 2).

ALKBH5 has been implicated in various physiological and pathophysiological processes. In mice, ALKBH5 is highly expressed in the testes where loss of ALKBH5 expression leads to decreased spermatogenesis, abnormal spermatozoa and increased apoptosis (3). In glioblastoma, ALKBH5 overexpression promotes stemness and tumor progression (2). In breast cancer cells under hypoxic conditions, HIF-1 alpha and HIF-2 alpha induce the expression of ALKH5 and leads to increased demethylation and expression of Nanog thus promoting breast cancer stem cell properties (4).


1. Chen, X. Y., Zhang, J., & Zhu, J. S. (2019). The role of m6A RNA methylation in human cancer. Molecular Cancer.

2. Deng, X., Su, R., Weng, H., Huang, H., Li, Z., & Chen, J. (2018). RNA N 6 -methyladenosine modification in cancers: Current status and perspectives. Cell Research.

3. Meyer, K. D., & Jaffrey, S. R. (2014). The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nature Reviews Molecular Cell Biology.

4. Zhang, C., Samanta, D., Lu, H., Bullen, J. W., Zhang, H., Chen, I., ... Semenza, G. L. (2016). Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m6A-demethylation of NANOG mRNA. Proceedings of the National Academy of Sciences of the United States of America.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ICC, KO, WB
Species: Hu
Applications: WB
Species: Hu
Applications: ELISA, AP, PA, WB
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Species: Hu
Applications: ELISA, AP, PA, WB
Species: Hu, Ma, Mu, Po, Rt
Applications: ChIP, DB, ICC/IF, WB
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA
Species: Hu
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Sh
Applications: ELISA, Flow, IHC, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ChIP, WB
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ChIP, ELISA, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, PLA, Simple Western, WB
Species: Hu
Applications: WB, ELISA, PA, AP

Publications for ALKBH5 Recombinant Protein (H00054890-P01) (0)

There are no publications for ALKBH5 Recombinant Protein (H00054890-P01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ALKBH5 Recombinant Protein (H00054890-P01) (0)

There are no reviews for ALKBH5 Recombinant Protein (H00054890-P01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ALKBH5 Recombinant Protein (H00054890-P01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ALKBH5 Products

Bioinformatics Tool for ALKBH5 Recombinant Protein (H00054890-P01)

Discover related pathways, diseases and genes to ALKBH5 Recombinant Protein (H00054890-P01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ALKBH5 Recombinant Protein (H00054890-P01)

Discover more about diseases related to ALKBH5 Recombinant Protein (H00054890-P01).

Pathways for ALKBH5 Recombinant Protein (H00054890-P01)

View related products by pathway.

PTMs for ALKBH5 Recombinant Protein (H00054890-P01)

Learn more about PTMs related to ALKBH5 Recombinant Protein (H00054890-P01).

Research Areas for ALKBH5 Recombinant Protein (H00054890-P01)

Find related products by research area.

Blogs on ALKBH5

There are no specific blogs for ALKBH5, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human ALKBH5 GST (N-Term) Protein and receive a gift card or discount.


Gene Symbol ALKBH5