Recombinant Human Alkaline Ceramidase 2 GST (N-Term) Protein

12.5% SDS-PAGE Stained with Coomassie Blue.

Product Discontinued

Recombinant Human Alkaline Ceramidase 2 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-226 of Human ASAH3L
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MCLFRQYATCFNSGIYLIWTLLVVVGIGSVYFHATLSFLGQMLDELAVLWVLMCALAMWFPRRYLPKIFRNDRGRFKVVVSVLSAVTTCLAFVKPAINNISLMTLGVPCTALLIAELKRCDNMRVFKLGLFSGLWWTLALFCWISDRAFCELLSSFNFPYLHCMWHILICLAAYLGCVCFAYFDAASEIPEQGPVIKFWPNEKWAFIGVPYVSLLCANKKSSVKIT

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: ACER2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 52.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Alkaline Ceramidase 2 GST (N-Term) Protein

• Acylsphingosine deacylase 3-like
• Alkaline CDase 2
• alkaline ceramidase 2
• AlkCDase 2
• ALKCDase2
• ASAH3L
• ceramide hydrolase
• EC 3.5.1.23
• FLJ41587
• haCER2
• N-acylsphingosine amidohydrolase 3-like

Background

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Regulates the maturation ofintegrin beta-1 (ITGB1) by controlling the generation of sphingosine in the Golgi complex. Inhibits cell adhesion byreducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems todepend on the balance between sphingosine and sphingosine-1-phosphate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ACER2