ALDH6A1 Antibody

Western Blot: ALDH6A1 Antibody [NBP1-52101] - Analysis of ALDH6A1 in Mouse Kidney lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ALDH6A1 Antibody Summary

• Description: Novus Biologicals Goat ALDH6A1 Antibody (NBP1-52101) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ALDH6A1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SRSSFRGDTN corresponding to internal region according to NP_005580.1.
• Epitope: C-SRSSFRGDTN
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ALDH6A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in human, mouse and rat kidney lysates (calculated MW of 57.8 kDa band according to NP_005580.1). IHC-P: Human liver shows textured staining of the cytoplasm consistent with mitochondria.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ALDH6A1 Antibody

• aldehyde dehydrogenase 6 family, member A1
• Aldehyde dehydrogenase family 6 member A1
• EC 1.2.1.18
• EC 1.2.1.27
• Malonate-semialdehyde dehydrogenase [acylating]
• malonate-semialdehyde dehydrogenase
• methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
• MGC40271
• MMSADHA
• MMSDHmitochondrial acylating methylmalonate-semialdehyde dehydrogenase

Background

This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALDH6A1 Antibody (NBP1-52101)(1)

Publication using NBP1-52101

• Alnouti Y, Klaassen CD. Tissue distribution, ontogeny, and regulation of aldehyde dehydrogenase (Aldh) enzymes mRNA by prototypical microsomal enzyme inducers in mice. Toxicol Sci 2008-01-01 [PMID: 17998271]

Bioinformatics

• Gene Symbol: ALDH6A1
• Entrez:
  • 4329(Human)
  • 104776(Mouse)
  • 81708(Rat)
• Uniprot:
  • NP_005580.1(Human)