ALDH2 Antibody (E4-D10)

Western Blot: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis on HepG2 cells lysate using anti-ALDH2 Mouse mAb.

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (E4-D10) [NBP2-67132] - Staining of PC-3M cells using anti-ALDH2 Mouse mAb.

Immunohistochemistry-Paraffin: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of paraffin- embedded mouse stomach tissue using anti-ALDH2 Mouse mAb.

Flow Cytometry: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of HepG2 cells with ALDH2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ALDH2 Antibody (E4-D10) [NBP2-67132] - Staining of HepG2 cells using anti-ALDH2 Mouse mAb.

Immunohistochemistry-Paraffin: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of paraffin- embedded human colon cancer tissue using anti-ALDH2 Mouse mAb.

Immunohistochemistry-Paraffin: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of paraffin- embedded human gastric cancer tissue using anti-ALDH2 Mouse mAb.

Immunohistochemistry-Paraffin: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of paraffin- embedded human liver cancer tissue using anti-ALDH2 Mouse mAb.

Immunohistochemistry-Paraffin: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of paraffin- embedded human lung cancer tissue using anti-ALDH2 Mouse mAb.

Immunohistochemistry-Paraffin: ALDH2 Antibody (E4-D10) [NBP2-67132] - Analysis of paraffin- embedded mouse colon tissue using anti-ALDH2 Mouse mAb.

Product Discontinued

ALDH2 Antibody (E4-D10) Summary

• Immunogen: Synthetic peptide within Human ALDH2 aa 468-517 / 517. (SwissProt: P05091 Human)
• Localization: Mitochondrion matrix
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for ALDH2 Antibody (E4-D10)

• acetaldehyde dehydrogenase 2
• aldehyde dehydrogenase 2 family (mitochondrial)
• aldehyde dehydrogenase, mitochondrial
• ALDH class 2
• ALDH2
• ALDH-E2
• ALDHI
• ALDM
• EC 1.2.1
• EC 1.2.1.3
• liver mitochondrial ALDH
• MGC1806
• nucleus-encoded mitochondrial aldehyde dehydrogenase 2

Background

ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALDH2