AKAP95/AKAP8 Antibody


Western Blot: AKAP95/AKAP8 Antibody [NBP1-79139] - Western blot analysis of AKAP95/AKAP8 expression in 1) A431 and 2) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-79139] - AKAP95/AKAP8 antibody was tested in HeLa cells at 1:500 with DyLight 488 (green). Nuclei and alpha Tubulin (NB100-690) were counterstained ...read more
Immunohistochemistry: AKAP95/AKAP8 Antibody [NBP1-79139] - IHC staining of AKAP95/AKAP8 in human ovarian cancer using DAB with hematoxylin counterstain.

Product Details

Product Discontinued
View other related AKAP95/AKAP8 Primary Antibodies

Order Details

    • Catalog Number
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

AKAP95/AKAP8 Antibody Summary

Human AKAP95/AKAP8 peptide within amino acids 650 to 692 [Swiss-Prot# O43823].
Nucleus matrix. Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
Nucleus without Nucleoli Marker
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This AKAP95/AKAP8 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. Use in Flow Cytometry reported in scientific literature (PMID: 16980585). In ICC/IF, nuclear staining was observed in HeLa cells. In Western Blot, a band can be seen at ~95 kDa representing AKAP95/AKAP8. In IHC-P, staining was observed in the nucleus, specifically nucleoli of human ovarian cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using
NBP1-79139 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole antisera
No Preservative

Alternate Names for AKAP95/AKAP8 Antibody

  • A kinase (PRKA) anchor protein 8
  • AKAP-8
  • AKAP-95 AKAP 95
  • A-kinase anchor protein 8
  • A-kinase anchor protein 95 kDa
  • A-kinase anchor protein, 95kDa
  • DKFZp586B1222


AKAP95 (A-kinase anchor protein 95 kD; murine gene - Akap8) is an anchoring protein which is responsible for the subcellular compartmentalization of PKA type II (cAMP-dependent protein kinase). AKAP95 localizes to nuclear matrix along with fidgetin and gets redistributed or detached from condensed chromatin during mitotic cell division. AKAP95 is expressed in heart, liver, skeletal muscle, kidney, pancreas etc. and shows a broad expression pattern overlapping with that of fidgetin during murine embryogenesis. AKAP95 can interact with fidgetin and binds to several proteins such as regulatory subunit of type II cAMP-dependent protein kinase (during mitosis), Eg7 of chromosome-condensing complex, p68 RNA helicase, c-Myc-binding protein, minichromosome maintenance 2 protein, and D-type cyclins as well as phosphodiesterases for serving diverse cellular functions including PKA signaling, DNA condensation, transcription, and DNA replication. Moreover, AKAP95 has recently been shown to implicate in cAMP-regulated mRNA stability as well as the nuclear translocation of active caspase 3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu, Rt, Am, Dr
Applications: WB, EM, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, IHC-FrFl, KO
Species: Hu
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P, IP
Species: Hu, Bt, Bv, Ca, Pm, Pm, Rb, Xp
Applications: IHC, IHC-P
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Po, Bv
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt, Bv, Fe
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for AKAP95/AKAP8 Antibody (NBP1-79139)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, ICC/IF, IP.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for AKAP95/AKAP8 Antibody (NBP1-79139) (0)

There are no reviews for AKAP95/AKAP8 Antibody (NBP1-79139). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for AKAP95/AKAP8 Antibody (NBP1-79139): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AKAP95/AKAP8 Antibody (NBP1-79139) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional AKAP95/AKAP8 Products

Bioinformatics Tool for AKAP95/AKAP8 Antibody (NBP1-79139)

Discover related pathways, diseases and genes to AKAP95/AKAP8 Antibody (NBP1-79139). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for AKAP95/AKAP8 Antibody (NBP1-79139)

Discover more about diseases related to AKAP95/AKAP8 Antibody (NBP1-79139).

Pathways for AKAP95/AKAP8 Antibody (NBP1-79139)

View related products by pathway.

PTMs for AKAP95/AKAP8 Antibody (NBP1-79139)

Learn more about PTMs related to AKAP95/AKAP8 Antibody (NBP1-79139).

Research Areas for AKAP95/AKAP8 Antibody (NBP1-79139)

Find related products by research area.

Blogs on AKAP95/AKAP8.

AKAP95/AKAP8 Orchestrates and Synchronizes Cellular Events
A-kinase anchor proteins (AKAPs), such as AKAP95/AKAP8, are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA). AKAPs orchestrate and synchronize cellular events by tethering the cAMP-dependent PKA and oth...  Read full blog post.

Coronavirus Brochure

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our AKAP95/AKAP8 Antibody and receive a gift card or discount.


Gene Symbol AKAP8