AKAP95/AKAP8 Antibody

Images

 
Western Blot: AKAP95/AKAP8 Antibody [NBP1-79139] - Western blot analysis of AKAP95/AKAP8 expression in 1) A431 and 2) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: AKAP95/AKAP8 Antibody [NBP1-79139] - AKAP95/AKAP8 antibody was tested in HeLa cells at 1:500 with DyLight 488 (green). Nuclei and alpha Tubulin (NB100-690) were counterstained ...read more
Immunohistochemistry: AKAP95/AKAP8 Antibody [NBP1-79139] - IHC staining of AKAP95/AKAP8 in human ovarian cancer using DAB with hematoxylin counterstain.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-79139
    • Availability
      Product Discontinued

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AKAP95/AKAP8 Antibody Summary

Immunogen
Human AKAP95/AKAP8 peptide within amino acids 650 to 692 [Swiss-Prot# O43823].
Localization
Nucleus matrix. Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.
Marker
Nucleus without Nucleoli Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AKAP8
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This AKAP95/AKAP8 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot, and Immunohistochemistry on paraffin-embedded sections. Use in Flow Cytometry reported in scientific literature (PMID: 16980585). In ICC/IF, nuclear staining was observed in HeLa cells. In Western Blot, a band can be seen at ~95 kDa representing AKAP95/AKAP8. In IHC-P, staining was observed in the nucleus, specifically nucleoli of human ovarian cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-79139 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for AKAP95/AKAP8 Antibody

  • A kinase (PRKA) anchor protein 8
  • AKAP-8
  • AKAP-95 AKAP 95
  • A-kinase anchor protein 8
  • A-kinase anchor protein 95 kDa
  • A-kinase anchor protein, 95kDa
  • DKFZp586B1222

Background

AKAP95 (A-kinase anchor protein 95 kD; murine gene - Akap8) is an anchoring protein which is responsible for the subcellular compartmentalization of PKA type II (cAMP-dependent protein kinase). AKAP95 localizes to nuclear matrix along with fidgetin and gets redistributed or detached from condensed chromatin during mitotic cell division. AKAP95 is expressed in heart, liver, skeletal muscle, kidney, pancreas etc. and shows a broad expression pattern overlapping with that of fidgetin during murine embryogenesis. AKAP95 can interact with fidgetin and binds to several proteins such as regulatory subunit of type II cAMP-dependent protein kinase (during mitosis), Eg7 of chromosome-condensing complex, p68 RNA helicase, c-Myc-binding protein, minichromosome maintenance 2 protein, and D-type cyclins as well as phosphodiesterases for serving diverse cellular functions including PKA signaling, DNA condensation, transcription, and DNA replication. Moreover, AKAP95 has recently been shown to implicate in cAMP-regulated mRNA stability as well as the nuclear translocation of active caspase 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP95/AKAP8 Antibody (NBP1-79139)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: FLOW, ICC/IF, IP.


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Product General Protocols

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ICC/IF Video Protocol

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Bioinformatics Tool for AKAP95/AKAP8 Antibody (NBP1-79139)

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Blogs on AKAP95/AKAP8.

AKAP95/AKAP8 Orchestrates and Synchronizes Cellular Events
A-kinase anchor proteins (AKAPs), such as AKAP95/AKAP8, are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA). AKAPs orchestrate and synchronize cellular events by tethering the cAMP-dependent PKA and oth...  Read full blog post.

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Bioinformatics

Gene Symbol AKAP8
Entrez
Uniprot