Recombinant Human AFAP His Protein Summary
Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 250-588 of Human AFAP Source: E.coli Uniprot ID: Q8N556 Amino Acid Sequence: GCSGPVDSE CPPPPSSPVH KAELEKKLSS ERPSSDGEGV VENGITTCNG KEQVKRKKSS KSEAKGTVSK VTGKKITKII SLGKKKPSTD EQTSSAEEDV PTCGYLNVLS NSRWRERWCR VKDNKLIFHK DRTDLKTHIV SIPLRGCEVI PGLDCKHPLT FRLLRNGQEV AVLEASSSED MGRWIGILLA ETGSSTDPEA LHYDYIDVEM SASVIQTAKQ TFCFMNRRVI SANPYLGGTS NGYAHPSGTA LHYDDVPCIN GSLRGKKPPV ASNGVTGKGK TLSSQPKKAD PAAVVKRTGS NAAQYKYGKN RVEADAKRLQ TKEEELLKRK EALRNRLAQL RK |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
AFAP1 |
Purity |
>80%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
Theoretical MW |
39.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA |
Preservative |
No Preservative |
Concentration |
0.5 mg/ml |
Purity |
>80%, by SDS-PAGE |
Alternate Names for Recombinant Human AFAP His Protein
Background
AFAP, or Actin filament-associated protein 1, contains a 81 kDa and a 90 kDa isoform, and is involved in the manipulation of actin filaments to form network or bundle structures and the regulation of cell-matrix linkage. Disease research is currently being conducted with AFAP and its relation to familial adenomatous polyposis, adenocarcinoma, breast cancer, prostate adenocarcinoma, prostatitis, polyposis, prostate cancer, and carcinoma. This protein interacts with PRKCB, PRKCD, PRKCG, ACTC1, and PRKCZ during the processes of signal transduction regulation and mechanical stimulus response.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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