Human AFAP ELISA Kit (Colorimetric)

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ELISA: Human AFAP ELISA Kit (Colorimetric) [NBP2-66655] - Samples were spiked with high concentrations of Human AFAP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human AFAP ELISA Kit (Colorimetric) [NBP2-66655] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

Human AFAP ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human AFAP1 in samples. No significant cross-reactivity or interference between Human AFAP1 and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.25%
Intra-Assay
CV% < 5.36%
Spike Recovery
86-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
AFAP1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human AFAP ELISA Kit (Colorimetric)

  • actin filament associated protein 1
  • AFAP
  • AFAP1
  • AFAP-110
  • FLJ56849

Background

AFAP, or Actin filament-associated protein 1, contains a 81 kDa and a 90 kDa isoform, and is involved in the manipulation of actin filaments to form network or bundle structures and the regulation of cell-matrix linkage. Disease research is currently being conducted with AFAP and its relation to familial adenomatous polyposis, adenocarcinoma, breast cancer, prostate adenocarcinoma, prostatitis, polyposis, prostate cancer, and carcinoma. This protein interacts with PRKCB, PRKCD, PRKCG, ACTC1, and PRKCZ during the processes of signal transduction regulation and mechanical stimulus response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AFAP1