ADI1 Overexpression Lysate

Images

 
Western Blot: ADI1 Overexpression Lysate (Adult Normal) [NBL1-07340] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADI1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ADI1 Overexpression Lysate Summary

Description
Transient overexpression lysate of acireductone dioxygenase 1 (ADI1) The lysate was created in HEK293T cells, using Plasmid ID RC200115 and based on accession number NM_018269. The protein contains a C-MYC/DDK Tag.
Gene
ADI1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ADI1 Overexpression Lysate

  • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
  • Acireductone dioxygenase (Ni(2+)-requiring)
  • acireductone dioxygenase 1
  • APL1
  • ARDsubmergence induced protein 2
  • EC 1.13.11.53
  • FLJ10913
  • HMFT1638
  • membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1
  • Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1
  • MTCBP1
  • MTCBP-1
  • Ni-ARD
  • SIPLMT1-MMP cytoplasmic tail-binding protein-1
  • Submergence-induced protein 2 homolog

Background

This gene encodes an enzyme that belongs to the aci-reductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. A pseudogene that is located on chromosome 20 has been defined for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Other Available Formats

NBL1-07340

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Bioinformatics

Gene Symbol ADI1