ADAR Knockout 293T Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
LYOPH

Order Details

ADAR Knockout 293T Cell Lysate Summary

Preparation
Method
Knockout achieved by using CRISPR/Cas9,17 bp deletion in exon 2 and 1 bp insertion in exon 2
Gene
ADAR

Applications/Dilutions

Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Concentration
LYOPH
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Type
Knockout 293T Cell
Life Stage
Embryonic
Tissue
Kidney

Notes

Powered by EDIGENE.
Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for ADAR Knockout 293T Cell Lysate

  • ADAR1DSH
  • adenosine deaminase, RNA-specific
  • DRADAP136
  • DSRADadenosine deaminase acting on RNA 1-A
  • EC 3.5.4
  • EC 3.5.4.-
  • G1P1double-stranded RNA-specific adenosine deaminase
  • IFI-4
  • IFI4dsRNA adenosine deaminase
  • interferon-induced protein 4
  • Interferon-inducible protein 4
  • K88DSRBP
  • p136,136 kDa double-stranded RNA-binding protein

Background

This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Other Available Formats

NBP2-66402

Additional ADAR Products

Array NBP2-66402

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Bioinformatics

Gene Symbol ADAR