Acylglycerol Kinase Antibody

Western Blot: Acylglycerol Kinase Antibody [NBP1-06013] - Analysis of Acylglycerol Kinase using NBP1-06013 at 1ug/ml in Human Brain (Substantia nigra) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Acylglycerol Kinase Antibody Summary

• Description: Novus Biologicals Goat Acylglycerol Kinase Antibody (NBP1-06013) is a polyclonal antibody validated for use in WB and ELISA. Anti-Acylglycerol Kinase Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CDPRKREQMLTSP corresponding to C-Terminus according to NP_060708.1.
• Epitope: CDPRKREQMLTSP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human brain (substantia nigra, hippocampus) lysates (calculated MW of 47.1 kDa band according to NP_060708.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26635614).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Acylglycerol Kinase Antibody

• acylglycerol kinase
• AGK
• EC 2.7.1.107
• EC 2.7.1.94
• FLJ10842
• FLJ10842
• hAGK
• HsMuLK
• mitochondrial
• MULK
• MULKhsMuLK
• Multi-substrate lipid kinase

Background

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Acylglycerol Kinase Antibody (NBP1-06013)(2)

Publications using NBP1-06013

• Daimon CM, Jasien JM, Wood WH et al. Hippocampal Transcriptomic and Proteomic Alterations in the BTBR Mouse Model of Autism Spectrum Disorder. Front Physiol. 2015-12-04 [PMID: 26635614] (WB, Mouse)
• Outeiro TF, Kontopoulos E, Altman S et al. Sirtuin 2 Inhibitors Rescue {alpha}-Synuclein-Mediated Toxicity in Models of Parkinson's Disease. Science 2007-06-21 [PMID: 17588900]

Bioinformatics

• Gene Symbol: AGK
• Entrez:
  • 55750(Human)
  • 69923(Mouse)
• Uniprot:
  • NP_060708.1(Human)