ACCN2 Overexpression Lysate


Western Blot: ACCN2 Overexpression Lysate [NBL1-07231] - Left-Control; Right -Over-expression Lysate for ACCN2

Product Details

Product Discontinued
View other related ACCN2 Lysates

Order Details

    • Catalog Number
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

ACCN2 Overexpression Lysate Summary

The lysate was created in HEK293T cells, using plasmid ID RC214490 and based on accession number NM_020039. The protein contains a C-terminal DDK tag.
C-terminal Myc/DDK
Homo sapiens amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
64.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ACCN2 Overexpression Lysate

  • Acid-sensing ion channel 1
  • acid-sensing ion channel 1a protein
  • amiloride-sensitive cation channel 2, neuronal
  • ASIC
  • BNAC2
  • BNaC2ACCN2 variant 3
  • Brain sodium channel 2
  • Cation channel, amiloride-sensitive, neuronal, 2
  • hBNaC2


This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products. [provided by RefSeq]. Transcript Variant: This variant (1) contains an insertion of 138 bps near the beginning of the region encoding the second hydrophobic transmembrane domain resulting in an additional 46 amino acids, as compared to variant 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PAGE, Flow-IC
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB, IHC, IP
Species: Hu, Mu, Rt, GP, Ze
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Mu
Applications: WB, Simple Western, IHC
Species: Hu
Applications: WB, IHC, IHC-P

Publications for ACCN2 Lysate (NBL1-07231) (0)

There are no publications for ACCN2 Lysate (NBL1-07231).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ACCN2 Lysate (NBL1-07231) (0)

There are no reviews for ACCN2 Lysate (NBL1-07231). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ACCN2 Lysate (NBL1-07231) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional ACCN2 Products

Bioinformatics Tool for ACCN2 Lysate (NBL1-07231)

Discover related pathways, diseases and genes to ACCN2 Lysate (NBL1-07231). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ACCN2 Lysate (NBL1-07231)

Discover more about diseases related to ACCN2 Lysate (NBL1-07231).

Pathways for ACCN2 Lysate (NBL1-07231)

View related products by pathway.

PTMs for ACCN2 Lysate (NBL1-07231)

Learn more about PTMs related to ACCN2 Lysate (NBL1-07231).

Blogs on ACCN2

There are no specific blogs for ACCN2, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our ACCN2 Overexpression Lysate and receive a gift card or discount.


Gene Symbol ASIC1
Novus 100% Guarantee