ACCN2 Overexpression Lysate

Product Discontinued

ACCN2 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC214490 and based on accession number NM_020039. The protein contains a C-terminal DDK tag.
• Epitope: C-terminal Myc/DDK
• Specificity: Homo sapiens amiloride-sensitive cation channel 2, neuronal (ACCN2), transcript variant 1, mRNA.
• Gene: ASIC1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 64.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ACCN2 Overexpression Lysate

• ACCN2
• Acid-sensing ion channel 1
• acid-sensing ion channel 1a protein
• amiloride-sensitive cation channel 2, neuronal
• ASIC
• ASIC1
• ASIC1ASIC1A
• BNaC2
• BNaC2ACCN2 variant 3
• Brain sodium channel 2
• Cation channel, amiloride-sensitive, neuronal, 2
• hBNaC2

Background

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown. Alternative splicing of this gene generates 2 transcript products. [provided by RefSeq]. Transcript Variant: This variant (1) contains an insertion of 138 bps near the beginning of the region encoding the second hydrophobic transmembrane domain resulting in an additional 46 amino acids, as compared to variant 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ASIC1