A2BP1 Antibody (1G10)

Product Discontinued

A2BP1 Antibody (1G10) Summary

• Immunogen: The N-terminal 100 amino acids of human A2BP1 expressed in and purified from E. coli. [UniProt# Q9NWB1]
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBFOX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Western Blot 1:1000-1:2000
• Application Notes: This A2BP1 (1G10) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~46 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 0.035% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for A2BP1 Antibody (1G10)

• 2BP1
• A2BP
• A2BP1 Hexaribonucleotide-binding protein 1
• Ataxin-2-binding protein 1
• FOX1 hexaribonucleotide binding protein 1
• Fox-1 homolog A
• FOX-1
• HRNBP1 ataxin 2-binding protein 1
• RNA binding protein fox-1 homolog 1
• RNA binding protein, fox-1 homolog (C. elegans) 1

Background

A2BP1, also known as Fox1 or ataxin-2 binding protein 1, is one of a family of 3 mammalian Fox1 homologues. A2BP1/Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of "Feminizing locus on X". The C. elegans A2BP1/Fox1 protein and its 3 mammalian homologues are all about 46 kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. As with the other Fox proteins, it is assumed that A2BP1/Fox1 has a role in the RNA splicing in the nervous system. Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm. Like Fox3, A2BP1/Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by A2BP1/Fox1 antibodies. Clone 1G10 was raised against the N-terminal 100 amino acids of human A2BP1/Fox1 as expressed in and purified from E. coli. Full length A2BP1/Fox1 was not used as the immunogen since the three mammalian Fox1 homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal regions of the three molecules are much more variable, so antibodies specific for each of the three molecules can therefore be generated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBFOX1
• Entrez:
  • 54715(Human)
• OMIM:
  • 605104(Human)
• Uniprot:
  • Q9NWB1(Human)