26S proteasome subunit 9 Antibody (3A4)

Western Blot: 26S proteasome subunit 9 Antibody (3A4) [H00005715-M01] - PSMD9 monoclonal antibody (M01), clone 3A4 Analysis of PSMD9 expression in HepG2.

ELISA: 26S proteasome subunit 9 Antibody (3A4) [H00005715-M01] - Detection limit for recombinant GST tagged PSMD9 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

26S proteasome subunit 9 Antibody (3A4) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSMD9 (AAH02383, 1 a.a. ~ 223 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDEEARQSGGSSQAGVVTVSDVQELMRRKEEIEAQIKANYDVLESQKGIGMNEPLVDCEGYPRSDVDLYQVRTARHNIICLQNDHKAVMKQVEEALHQLHARDKEKQARDMAEAHKEAMSRKLGQSESQGPPRAFAKVNSISPGSPASIAGLQVDDEIVEFGSVNTQNFQSLHNIGSVVQHSEGKPLNVTVIRRGEKHQLRLVPTRWAGKGLLGCNIIPLQR
• Specificity: PSMD9 - proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMD9
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for 26S proteasome subunit 9 Antibody (3A4)

• 26S proteasome non-ATPase regulatory subunit 9
• p27
• proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• Rpn4

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMD9
• Entrez:
  • 5715(Human)
• OMIM:
  • 603146(Human)
• Uniprot:
  • AAH02383(Human)