| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Low Endotoxin, Azide and BSA Free | NB600-1443-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1182SS | |||
| Azide and BSA Free | NBP2-80721 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP2-52716AFP488 | |||
| Alexa Fluor Plus 555 | NBP2-52716AFP555 | |||
| Alexa Fluor Plus 594 | NBP2-52716AFP594 | |||
| Alexa Fluor Plus 647 | NBP2-52716AFP647 | |||
| Alexa Fluor Plus 680 | NBP2-52716AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52716-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-9287-0.025mg | |||
![Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. HeLa; 3. Jurkat; 4. Daudi; 5. HEK 293; 6. Rh30; 7. A375; 8. T98G; 9. HCT-116; 10. Hep-2.](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Cell-Lines-SDS-Page-NBP2-30114-img0001.jpg "Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. HeLa; 3. Jurkat; 4. Daudi; 5. HEK 293; 6. Rh30; 7. A375; 8. T98G; 9. HCT-116; 10. Hep-2.")
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0003.jpg "Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in HCa-1 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.")
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in HCa-1 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0001.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro. HCa-1 and MIH-2 cells were injected intramuscularly into the right thighs of the mice, and tumors were irradiated with 10 Gy of radiation. Shown are IL-12 and DC (CD11c+) expressions at 1, 3, and 7 days after irradiation in MIH-2 tumors in the spleens of HCa-1 bearing mice (** P < 0.01). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0002.jpg)
![Immunohistochemistry: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo and DCs in vitro.IL-6 and IL-12 were measured at 1, 3, and 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 and IL-12 in MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice and stimulated with LPS. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0146463), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-12-Antibody-Immunohistochemistry-NB600-1443-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-31020241616187.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416182614.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation-induced IL-6 suppressed IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.IL-6 & IL-12 were measured at 1, 3, & 7 days after 10 Gy irradiation of tumors. Shown are the expressions of IL-6 & IL-12 in (a) HCa-1 & (b) MIH-2 tumors (* P < 0.05). BMDCs were prepared from bone marrow of mice & stimulated with LPS. (c) IL-6 expression analyzed at the indicated times by ELISA in irradiated or non-irradiated BMDCs in vitro. (d) IL-6R (** P < 0.01, vs No IR) & (e) sIL6R (** P < 0.01) were evaluated at 2 day after 10 Gy irradiation to BMDCs by FACS & ELISA, respectively. BMDCs were treated without or with IL-6 (20 ng/mL) plus LPS. (f) Down regulation of IL-12 production by IL-6 treatment in BMDCs (** P < 0.01). BMDCs were stimulated with LPS for activation & cultured with or without anti-IL-6 antibody (final concentration of 500 ng/mL) during the indicated times after irradiation in vitro. IL-6 & IL-12 were analyzed by ELISA. (g) Recovery of IL-12 production & (h) B7-1/ B7-2 expression using an anti-IL-6 antibody in irradiated-BMDCs stimulated with LPS in vitro. (** P < 0.01, * P < 0.05). Data are from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416154619.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-12 Antibody [NB600-1443] - Irradiation inhibited IL-12 expression in tumors of hepatoma-bearing mice in vivo & DCs in vitro.HCa-1 & MIH-2 cells were injected intramuscularly into the right thighs of the mice, & tumors were irradiated with 10 Gy of radiation. Shown are IL-12 & DC (CD11c+) expressions at 1, 3, & 7 days after irradiation in (a) HCa-1 & (b) MIH-2 tumors & (c) IL-12 expression in the spleens of HCa-1 bearing mice (** P < 0.01). (d) IL-12 expression in BMDCs incubated with supernatant of MIH-2 tumor cells or supernatant of 10 Gy irradiating MIH-2 tumor cells. (** P < 0.01). DCs were differentiated from bone marrow of C3H/HeN normal mice (BMDCs). These were incubated for 72 h in supernatant of MIH-2 tumor cells with or without 10 Gy irradiation. IL-12 was intracellular stained with IL-12 antibody & analyzed by FACS. BMDCs also were stimulated with LPS (100 ng/mL) to allow maturation & irradiated with 10 Gy of radiation. (e) IL-12 expression (** P < 0.01), (f) cell viability, & (g) B7.1 expression in LPS stimulated BMDCs receiving 10 Gy radiation. (* P < 0.05). Data are from three independent experiments with five mice per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26745884), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-1443_goat-polyclonal-il-12-antibody-310202416163756.jpg)
![Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Western-Blot-H00004218-M02-img0003.jpg "Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: RAB8A Antibody (3G1) [H00004218-M02] - Neuromast hair cells of zebrafish embryos stained with RAB8A antibody. Donkey anti-mouse Alexa Fluor 594 secondary antibody was used. Image from verified customer.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-Immunocytochemistry-Immunofluorescence-H00004218-M02-img0005.jpg)
![ELISA: RAB8A Antibody (3G1) [H00004218-M02] - Detection limit for recombinant GST tagged RAB8A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAB8A-Antibody-3G1-ELISA-H00004218-M02-img0006.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0008.jpg "Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.")
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of NALM-6 human pre-B cell leukemia cell line Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0001.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD9 (MEM-61) Dy161.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0002.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0003.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) APC-CyTM7.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0004.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Surface staining of human peripheral blood with anti-CD9 (MEM-61) Pacific BlueTM.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0005.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) APC.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0006.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) PE.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-Flow-Cytometry-NB500-327-img0007.jpg)
![CyTOF-ready: CD9 Antibody (MEM-61) [NB500-327] - Analysis of CD9 antibody on human tissue. Scale, 50um. Image from verified customer review.](http://images.novusbio.com/fullsize/CD9-Antibody-MEM-61-CyTOF-ready-NB500-327-img0009.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Immunohistochemistry-Paraffin-NB600-1182-img0009.jpg "Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.")
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Western-Blot-NB600-1182-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Immunocytochemistry-Immunofluorescence-NB600-1182-img0016.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue with 1:500 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The staining was primarily localized to the membranes and inter-cellular spaces of the cancer cells. Staining intensity for 1:500 dilution was lower than what was seen in sections tested with 1:100 dilution. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Immunohistochemistry-Paraffin-NB600-1182-img0014.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Using the PE direct conjugate A cell surface stain was performed on HT-29 cells with EpCAM (AUA1) antibody NB600-1182PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Flow-Cytometry-NB600-1182-img0008.jpg)
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of A431 lysate (A) and Hek293 lysate (B) using EpCAM/CD326 antibody (AUA1) at 2 ug/ml. Non-reduced lysate was used in each lane.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Western-Blot-NB600-1182-img0001.jpg)
![Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using EpCAM/CD326 antibody (blue) and matched Isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/EpCAM-TROP1-Antibody-AUA1-Flow-Cytometry-NB600-1182-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0006.jpg "Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.")
![Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human tonsil tissue.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunohistochemistry-Paraffin-NB300-511-img0003.jpg)
![Flow Cytometry: CFTR Antibody (CF3) [NB300-511] - Human airway epithelial cells stained with CFTR (CF3) coupled with goat anti-mouse FITC conjugated secondary antibody. Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Flow-Cytometry-NB300-511-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - Staining of human pancreas cryosection (200x). Alpha cells are strongly labeled. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CFTR Antibody (CF3) [NB300-511] - CFTR staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CFTR at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight 488 conjugated antibody.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunocytochemistry-Immunofluorescence-NB300-511-img0005.jpg)
![Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Cancer biopsies of deparaffinized Human colon carcinoma tissue.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunohistochemistry-Paraffin-NB300-511-img0001.jpg)
![Immunohistochemistry-Paraffin: CFTR Antibody (CF3) [NB300-511] - Normal biopsies of deparaffinized Human pancreas tissue.](http://images.novusbio.com/fullsize/CFTR-Antibody-CF3-Immunohistochemistry-Paraffin-NB300-511-img0002.jpg)
![Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].](http://images.novusbio.com/fullsize/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.](http://images.novusbio.com/fullsize/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg)
![Western Blot: AICDA Antibody [NB100-93454] - Staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AICDA-Antibody-Western-Blot-NB100-93454-img0001.jpg "Western Blot: AICDA Antibody [NB100-93454] - Staining of Human Tonsil lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Cytokeratin 8 Antibody (AE3) [NB120-9287] - An intracellular stain was performed on A431 cells with Cytokeratin 8 Antibody [AE3] NB120-9287AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytokeratin-8-Antibody-AE3-Flow-Cytometry-NB120-9287-img0001.jpg "Flow Cytometry: Cytokeratin 8 Antibody (AE3) [NB120-9287] - An intracellular stain was performed on A431 cells with Cytokeratin 8 Antibody [AE3] NB120-9287AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - PMA and Ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-PACBLU.](http://images.novusbio.com/fullsize/CD69-Antibody-H1-2F3-Flow-Cytometry-NBP1-28011-img0003.jpg "Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - PMA and Ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-PACBLU.")