Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. ...read more
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Cell Lines IMB-105 is a simple and fast solution for screening proteins from various human cell lines. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-BlotT...8;, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
INSTA-Blot͐ Human Cell Lines NBP2-30114 contains denatured proteins from human cell line lysates (See Cell Lysate Preparation) loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot is sold ready-to-use in your western blot protocol.
Human cell lines are lysed in 1X lysis buffer (10 mM Tris, pH 8.0,130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the cell lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).Use in Western blot reported in scientific literature (PMID: 24842606)
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