INSTA-Blot Human Cell Lines

Western Blot: INSTA-Blot Human Cell Lines [NBP2-30114] - Human Cell Lines. Approximately 20 ug per lane of human cell line lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. HeLa; 3. Jurkat; 4. Daudi; 5. HEK 293; 6. Rh30; 7. A375; 8. T98G; 9. HCT-116; 10. Hep-2.

• Reactivity: Hu
• Applications: WB

INSTA-Blot Human Cell Lines Summary

• Description: Mark12 is used as the molecular weight marker for this product.
• Background: INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Cell Lines IMB-105 is a simple and fast solution for screening proteins from various human cell lines. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-BlotT...8;, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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• Preparation Method: INSTA-Blot͐ Human Cell Lines NBP2-30114 contains denatured proteins from human cell line lysates (See Cell Lysate Preparation) loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot is sold ready-to-use in your western blot protocol.

Applications/Dilutions

• Dilutions:
  • Western Blot reported in scientific literature (PMID 24842606)
• Application Notes: Human cell lines are lysed in 1X lysis buffer (10 mM Tris, pH 8.0,130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the cell lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).Use in Western blot reported in scientific literature (PMID: 24842606)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24842606)

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Notes

Mark12 is used as the molecular weight marker for this product.

Alternate Names for INSTA-Blot Human Cell Lines

• INSTA Blot Human Cell Lines
• INSTABlot Human Cell Lines
• INSTA-Blot Human

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.

Publications for INSTA-Blot Human Cell Lines (NBP2-30114)(3)

Publications using NBP2-30114

• Tai TT, Mu A, Adachi Y et al. Neurotransmitter transporter family including SLC6A6 and SLC6A13 contributes to the 5-aminolevulinic acid (ALA)-induced accumulation of protoporphyrin IX and photo-damage, through uptake of ALA by cancerous cells. Photochem. Photobiol. 2014-05-20 [PMID: 24842606] (WB, Human)
  
  Details:
  The Human Cell Line Blot was used to probe for SLC6A6 and SLC6A13 protein expression, Fig 5.
• Takahashi A, Tokita H, Takahashi K et al. A novel potent tumour promoter aberrantly overexpressed in most human cancers. Sci Rep. 2011-01-01 [PMID: 22355534] (WB, Human)
  
  Details:
  INSTA-Blot Human Cell Lines (IMB-105). WB: The membrane was probed with the FEAT antibody, Fig 2A.
• Hayashi Reiko, Goto Yuya, Ikeda Ryuji et al. CDCA4 is an E2F transcription factor family-induced nuclear factor that regulates E2F-dependent transcriptional activation and cell proliferation. J Biol Chem. 2006-11-24 [PMID: 16984923] (WB, Human)