IL-1 beta/IL-1F2 Antibodies

501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL." class="big_lightbox"> 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL." alt="Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB501). The ND₅₀ is typically 10-30 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB6811). The ND₅₀ is typically 4-15 µg/mL." class="big_lightbox"> 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB6811). The ND₅₀ is typically 4-15 µg/mL." alt="Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB6811). The ND₅₀ is typically 4-15 µg/mL." class="big_thumb" />

681-PI) was serially diluted and captured by Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 681-PI) was serially diluted and captured by Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF007). A specific band was detected for IL-1 beta /IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox"> HAF007). A specific band was detected for IL-1 beta /IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta /IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-1 beta /IL-1F2 Polyclonal Antibody (Catalog # AB-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta /IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta / IL-1F2 (50 pg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Poly-clonal Antibody (Catalog # AB-201-NA). The ND₅₀ is typically 0.05-0.1 µg/mL." class="big_lightbox"> 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta / IL-1F2 (50 pg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL-1F2 Poly-clonal Antibody (Catalog # AB-201-NA). The ND₅₀ is typically 0.05-0.1 µg/mL." class="big_thumb" />

1796-FL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796). The ND₅₀ is typically 0.02-0.08 µg/mL." class="big_lightbox"> 1796-FL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796). The ND₅₀ is typically 0.02-0.08 µg/mL." alt="Recombinant Feline IL-1 beta /IL-1F2 (Catalog # 1796-FL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796). The ND₅₀ is typically 0.02-0.08 µg/mL." class="big_thumb" />

1796-FL) was serially diluted and captured by Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1796-FL) was serially diluted and captured by Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1796-FL) was serially diluted and captured by Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1796-FL) was serially diluted and captured by Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Feline IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND₅₀ is typically 4-20 µg/mL." class="big_lightbox"> 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND₅₀ is typically 4-20 µg/mL." alt="Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681). The ND₅₀ is typically 4-20 µg/mL." class="big_thumb" />

681-PI) was serially diluted and captured by Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 681-PI) was serially diluted and captured by Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF681) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-1 beta /IL-1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB33401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB681). The ND₅₀ is typically 1-3 µg/mL." class="big_lightbox"> 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB681). The ND₅₀ is typically 1-3 µg/mL." alt="Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB681). The ND₅₀ is typically 1-3 µg/mL." class="big_thumb" />

3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3747). The ND₅₀ is typically 2-10 µg/mL." class="big_lightbox"> 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3747). The ND₅₀ is typically 2-10 µg/mL." alt="Recombinant Canine IL-1 beta /IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3747). The ND₅₀ is typically 2-10 µg/mL." class="big_thumb" />