IL-1 beta/IL-1F2 Antibodies
Interleukin-1 beta (IL-1 beta), also called IL-1F2, is a proinflammatory cytokine expressed by cells of the immune system that plays a role in host defense following injury or inflammation. The inactive, pro-form of the human IL-1 beta protein is 269 amino acids (aa) in length with a theoretical molecular weight (MW) of 31 kDa, whereas the cleaved, active form is 18 kDa. We offer IL-1 beta/IL-1F2 Antibodies in our catalog have been cited in over 500+ publications with multiple 4- and 5-star customer reviews. Browse our inventory of recombinant, monoclonal, polyclonal, and IL-1 beta-conjugated antibodies. Validated research applications for our IL-1 beta/IL-1F2 antibodies include Blocking/Neutralizing, CyTOF-ready, Dual RNAscope ISH-IHC, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Simple Western, and Western Blot. These IL-1 beta antibodies are reactive in a range of species such as Human, Mouse, Rat, Canine, Equine, Porcine, Rabbit, and more. Choose the anti-IL-1 beta antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products. Each IL-1 beta/IL-1F2 antibody is fully covered by Novus’ Guarantee+ to give you peace of mind and support when you need it.
Alternate Names for IL-1 beta/IL-1F2 Antibodies
anti-IL-1 beta/IL-1F2 antibody, anti-IL1B antibody, anti-catabolin antibody, anti-IL-1 antibody, anti-IL-1 beta antibody, anti-IL-1B antibody, anti-IL1-BETA antibody, anti-IL1F2IL-1 beta antibody, anti-interleukin 1, beta antibody, anti-interleukin-1 beta antibody, anti-preinterleukin 1 beta antibody, anti-pro-interleukin-1-beta antibody
54 Results for "il-1-beta-il-1f2" in Primary Antibodies
![Western Blot: IL-1 beta/IL-1F2 Antibody (43N1G6) [NBP2-27342] - Analysis of IL-1beta in A) full-length recombinant protein and B) human spleen lysate using IL-1beta antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-43N1G6-Western-Blot-NBP2-27342-img0002.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (43N1G6) [NBP2-27342] - Analysis of IL-1beta in A) full-length recombinant protein and B) human spleen lysate using IL-1beta antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of paraffin-embedded human kidney tissue using anti-IL1 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunohistochemistry-Paraffin-NBP2-67865-img0004.jpg)
![Flow Cytometry: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Analysis of Hela cells with IL1 beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Flow-Cytometry-NBP2-67865-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IL-1 beta/IL-1F2 Antibody (JJ087-3) [NBP2-67865] - Staining IL1 beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-JJ087-3-Immunocytochemistry-Immunofluorescence-NBP2-67865-img0002.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody [NBP3-11364] - Recombinant Rabbit IL-1 beta/IL-1F2 detected using Chicken anti Rabbit IL-1 beta/IL-1F2 as the capture reagent and Chicken anti Rabbit IL-1 beta/IL-1F2 beta:Biotin as the detection reagent followed by Streptavidin:HRP.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-ELISA-NBP3-11364-img0001.jpg "ELISA: IL-1 beta/IL-1F2 Antibody [NBP3-11364] - Recombinant Rabbit IL-1 beta/IL-1F2 detected using Chicken anti Rabbit IL-1 beta/IL-1F2 as the capture reagent and Chicken anti Rabbit IL-1 beta/IL-1F2 beta:Biotin as the detection reagent followed by Streptavidin:HRP.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (PS01-39) [NBP3-32449] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32449) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32449_rabbit-il-1-beta-il-1f2-mab-ps01-39-278202415212445.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (PS01-39) [NBP3-32449] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32449) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")