IL-1 beta/IL-1F2 Antibodies

3340-EL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-1 beta /IL-1F2 (100 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-1 beta /IL-1F2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3340). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox"> 3340-EL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-1 beta /IL-1F2 (100 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-1 beta /IL-1F2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3340). The ND₅₀ is typically 0.05-0.25 µg/mL." alt="Recombinant Equine IL-1 beta / IL-1F2 (Catalog # 3340-EL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Equine IL-1 beta /IL-1F2 (100 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IL-1 beta /IL-1F2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3340). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1009-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1 beta /IL-1F2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1009). The ND₅₀ is typically 0.4-1.2 µg/mL. " class="big_lightbox"> 1009-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1 beta /IL-1F2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1009). The ND₅₀ is typically 0.4-1.2 µg/mL. " alt="Recombinant Cotton Rat IL-1 beta /IL-1F2 (1009-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-1 beta /IL-1F2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-1 beta / IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1009). The ND₅₀ is typically 0.4-1.2 µg/mL. " class="big_thumb" />

F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood monocytes treated with 500 ng/mL LPS overnight, were either (A) stained or (B) not stained with Human anti-Human IL-1 beta/IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody (Catalog # MAB10349) followed by APC-conjugated anti-Human IgG secondary antibody (F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF37471). The ND₅₀ is typically 3-18 ng/mL." class="big_lightbox"> 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF37471). The ND₅₀ is typically 3-18 ng/mL." alt="Recombinant Canine IL-1 beta /IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF37471). The ND₅₀ is typically 3-18 ng/mL." class="big_thumb" />

F0111) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0111) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood monocytes, either (A) untreated, or (B) treated with 500 ng/mL LPS overnight, were stained with Rabbit anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB8406) followed by APC-conjugated anti-Rabbit IgG secondary antibody (Catalog # F0111) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

AR002). Under these conditions, proliferation elicited by IL-1 beta /IL-1F2 is neutralized (green line) by increasing concen-trations of Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB8049). The ND₅₀ is typically 2-10 µg/mL." class="big_lightbox"> AR002). Under these conditions, proliferation elicited by IL-1 beta /IL-1F2 is neutralized (green line) by increasing concen-trations of Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB8049). The ND₅₀ is typically 2-10 µg/mL." alt="Recombinant Equine IL-1 beta /IL-1F2 induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of concanavalin A (1.25 µg/mL) in a dose-depend-ent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-1 beta /IL-1F2 is neutralized (green line) by increasing concen-trations of Mouse Anti-Equine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB8049). The ND₅₀ is typically 2-10 µg/mL." class="big_thumb" />