CXCL1/GRO alpha/KC/CINC-1 Antibodies

453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox"> 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453). The ND₅₀ is typically 0.05-0.25 µg/mL." alt="Recombinant Mouse CXCL1/GROa/KC/ CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

453-KC) was serially diluted and captured by Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 453-KC) was serially diluted and captured by Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox"> 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND₅₀ is typically 0.3-1.5 µg/mL." alt="Recombinant Mouse CXCL1/KC (453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL1/KC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

453-KC) was serially diluted and captured by Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 453-KC) was serially diluted and captured by Rat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB453) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-453-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." class="big_lightbox"> MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." alt="Human peripheral blood mononuclear cell (PBMCs) treated with 1 µg/mL LPS for 24 hours and 3 µM monensin for 2 hours was stained with Mouse Anti-Human/Primate CXCL1/GROa/ KC/CINC-1 Monoclonal Antibody (Catalog # MAB275, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)." class="big_thumb" />

275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GROa/KC/CINC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB275). The ND₅₀ is typically 0.600 - 9.00 µg/mL." class="big_lightbox"> 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Primate CXCL1/GROa/KC/CINC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB275). The ND₅₀ is typically 0.600 - 9.00 µg/mL." class="big_thumb" />

275-GR) was serially diluted and captured by Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 275-GR) was serially diluted and captured by Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

275-GR), Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (453-KC), Recombinant Rat CXCL1/GROa/KC/CINC-1 (515-CN), Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 aa 35-107 (276-GB), Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 (277-GG), Recombinant Human CXCL7/NAP-2 (393-NP), and Recombinant Human CXCL4/PF4 (795-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 275-GR), Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (453-KC), Recombinant Rat CXCL1/GROa/KC/CINC-1 (515-CN), Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 aa 35-107 (276-GB), Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 (277-GG), Recombinant Human CXCL7/NAP-2 (393-NP), and Recombinant Human CXCL4/PF4 (795-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human CXCL1/GROa/KC/CINC-1 (275-GR), Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (453-KC), Recombinant Rat CXCL1/GROa/KC/CINC-1 (515-CN), Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 aa 35-107 (276-GB), Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 (277-GG), Recombinant Human CXCL7/NAP-2 (393-NP), and Recombinant Human CXCL4/PF4 (795-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL1/GROa (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GROa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275). The ND₅₀ is typically 0.03-0.15 µg/mL." class="big_lightbox"> 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL1/GROa (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/GROa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275). The ND₅₀ is typically 0.03-0.15 µg/mL." class="big_thumb" />

275-GR) was serially diluted and captured by Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 275-GR) was serially diluted and captured by Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL1/GRO alpha /KC/CINC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF275) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_lightbox"> 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND₅₀ is typically 0.15-0.75 µg/mL." alt="Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND₅₀ is typically0.05-0.25 µg/mL." class="big_lightbox"> 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND₅₀ is typically0.05-0.25 µg/mL." alt="Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (Catalog # 453-KC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/GROa/KC/CINC-1 aa 20-96 (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB453R). The ND₅₀ is typically0.05-0.25 µg/mL." class="big_thumb" />

515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/ CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB515). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox"> 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/ CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB515). The ND₅₀ is typically 3-15 µg/mL." alt="Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/ CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB515). The ND₅₀ is typically 3-15 µg/mL." class="big_thumb" />

HAF008). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Rat CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB5151) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB5151). The ND₅₀ is typically 0.075-45 µg/mL." class="big_lightbox"> 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB5151). The ND₅₀ is typically 0.075-45 µg/mL." class="big_thumb" />

F0110) and Rat Anti-Mouse TNF-a Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_lightbox"> F0110) and Rat Anti-Mouse TNF-a Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." alt="Mouse splenocytes either (A) treated with 1 µg/mL LPS and Brefeldin A for 4 hours or (B) untreated were stained with Rabbit Anti-Mouse CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB4532) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Rat Anti-Mouse TNF-a Fluorescein-conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_thumb" />