CXCL1/GRO alpha/KC/CINC-1 Antibodies

MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) treated with 1 µg/mL LPS for 24 hours and 3 µM monensin for 2 hours were stained with Recombinant Mouse Anti-Human/Primate CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB275R, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/ GROa/KC/CINC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Primate CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB275R). The ND₅₀ is typically 0.600 - 9.00 µg/mL." class="big_lightbox"> 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/ GROa/KC/CINC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Primate CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB275R). The ND₅₀ is typically 0.600 - 9.00 µg/mL." class="big_thumb" />