Fibroblast Activation Protein alpha/FAP Antibody (983802) [Alexa Fluor™ Plus 647]

• Reactivity: Mu
• Clone: 983802
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 647

Fibroblast Activation Protein alpha/FAP Antibody (983802) [Alexa Fluor™ Plus 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Fibroblast Activation Protein alpha /FAP
• Specificity: Detects mouse Fibroblast Activation Protein alpha /FAP in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibroblast Activation Protein alpha/FAP Antibody (983802) [Alexa Fluor™ Plus 647]

• 170 kDa melanoma membrane-bound gelatinase
• DKFZp686G13158
• DPPIV
• EC 3.4.21.-
• FAP
• FAPA
• Fibroblast Activation Protein alpha
• fibroblast activation protein, alpha
• Integral membrane serine protease
• Seprase
• vibronectin

Background

FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26) (1, 2). Within the extracellular domain, mouse FAP shares 90% and 97% amino acid (aa) sequence identity with human and rat FAP, respectively (3, 4). Alternative splicing of mouse FAP generates isoforms with a 33 aa or 5 aa deletion in the extracellular juxtamembrane region (3). FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis (1, 5-7). It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences (5, 8). FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin (1, 5, 8) as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins) (9). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface (5, 8, 10, 11). The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion (10-13). In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity (14).

1. Zi, F. et al. (2015) Mol. Med. Rep. 11:3203.
2. Pineiro-Sanchez, M.L. et al. (1997) J. Biol. Chem. 272:7595.
3. Niedermeyer, J. et al. (1997) Int. J. Cancer 71:383.
4. Scanlan, M.J. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5657.
5. Park, J.E. et al. (1999) J. Biol. Chem. 274:36505.
6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3110.
7. Bauer, S. et al. (2006) Arthritis Res. 8:R171.
8. Aertgeerts, K. et al. (2005) J. Biol. Chem. 280:19441.
9. Keane, F.M. et al. (2011) FEBS J. 278:1316.
10. Ghersi, G. et al. (2006) Cancer Res. 66:4652.
11. Ghersi, G. et al. (2002) J. Biol. Chem. 277:29231.
12. Cheng, J.D. et al. (2005) Mol. Cancer Ther. 4:351.
13. Cheng, J.D. et al. (2002) Cancer Res. 62:4767.
14. Kraman, M. et al. (2010) Science 330:827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.