| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89338-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43889 | |||
| BSA Free | NBP2-34477-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43887 | |||
| BSA Free | NBP2-34475-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54587-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73237 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB18382-SP | |||
![Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Western-Blot-NBP2-89338-img0002.jpg "Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa")
![Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDa](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-056-Immunoprecipitation-NBP2-89338-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0006.jpg "Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody at 1/1,000 dilution. Lane 1: Human heart tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 100 kDa Observed band size: 130 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Staining Pan-Cadherin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunocytochemistry-Immunofluorescence-NBP2-67540-img0002.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Pan-Cadherin antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodfor 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Hela cells with Pan-Cadherin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Flow-Cytometry-NBP2-67540-img0001.jpg)
![Western Blot: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of Pan-Cadherin on different lysates using anti-Pan-Cadherin antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart Lane 2: Mouse heart Lane 3: Rat heart](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Western-Blot-NBP2-67540-img0005.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0003.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue using anti-Pan-Cadherin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0004.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0007.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (ST54-01) [NBP2-67540] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Pan-Cadherin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-ST54-01-Immunohistochemistry-Paraffin-NBP2-67540-img0008.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: E-Cadherin Antibody (CL1180) [NBP2-34477] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Western-Blot-NBP2-34477-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunocytochemistry-Immunofluorescence-NBP2-34477-img0015.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0010.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0011.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human colorectal cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0012.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1180) [NBP2-34477] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1180-Immunohistochemistry-Paraffin-NBP2-34477-img0013.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining in human duodenum and cerebral cortex tissues. Corresponding CDH1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: E-Cadherin Antibody (CL1170) [NBP2-34475] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Western-Blot-NBP2-34475-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of BEWO cells using the Anti-CDH1 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunocytochemistry-Immunofluorescence-NBP2-34475-img0015.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0010.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0011.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human colorectal cancer shows moderate to strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0012.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (CL1170) [NBP2-34475] - Staining of human cerebral cortex shows no positivity in neuronal cells as expected.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-CL1170-Immunohistochemistry-Paraffin-NBP2-34475-img0013.jpg)
![Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Western-Blot-NBP2-53229-img0005.jpg "Western Blot: E-Cadherin Antibody (4A2) [NBP2-53229] - Western Blot Analysis of MCF-7 cell lysate using E-Cadherin Antibody (4A2).")
![Immunocytochemistry/Immunofluorescence: E-Cadherin Antibody (4A2) [NBP2-53229] - Immunofluorescence analysis of MeOH-fixed MCF-7 cells. E-Cadherin Mouse Monoclonal Antibody (4A2) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Immunocytochemistry-Immunofluorescence-NBP2-53229-img0007.jpg)
![Immunohistochemistry: E-Cadherin Antibody (4A2) [NBP2-53229] - Staining of human pancreatic tumor. Image from a verified customer review.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Immunohistochemistry-NBP2-53229-img0003.jpg)
![Flow Cytometry: E-Cadherin Antibody (4A2) [NBP2-53229] - Flow Cytometric Analysis of human trypsinized MCF-7 cells using E-Cadherin Antibody (4A2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Flow-Cytometry-NBP2-53229-img0006.jpg)
![Immunohistochemistry-Paraffin: E-Cadherin Antibody (4A2) [NBP2-53229] - Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (4A2).](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-4A2-Immunohistochemistry-Paraffin-NBP2-53229-img0001.jpg)
![Western Blot: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin.](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Western-Blot-NBP2-46200-img0011.jpg "Western Blot: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin.")
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0010.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0001.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0002.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0003.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0004.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0005.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0006.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0007.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0008.jpg)
![Immunohistochemistry: pan Cadherin Antibody (4F1) [NBP2-46200] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/pan-Cadherin-Antibody-4F1-Immunohistochemistry-NBP2-46200-img0009.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0003.jpg "Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) PE.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0001.jpg)
![Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) APC.](http://images.novusbio.com/fullsize/E-Cadherin-Antibody-67A4-Flow-Cytometry-NBP1-42793-img0002.jpg)
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Western-Blot-NBP3-12922-img0004.jpg "Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. MCF-7 whole cell extract (30 ug) with the indicated treatments were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Western-Blot-NBP3-12922-img0001.jpg)
![Western Blot: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - E-Cadherin (phospho Ser838/840) antibody detects E-Cadherin (phospho Ser838/840) protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin (phospho Ser838/840) antibody (NBP3-12922) diluted at 1:10000.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Western-Blot-NBP3-12922-img0002.jpg)
![Immunoprecipitation: E-Cadherin [p Ser838, p Ser840] Antibody [NBP3-12922] - Immunoprecipitation of E-Cadherin (phospho Ser838/840) protein from MCF-7 whole cell extracts using 5 ug of E-Cadherin (phospho Ser838/840) antibody (NBP3-12922). Western blot analysis was performed using E-Cadherin (phospho Ser838/840) antibody (NBP3-12922). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/E-Cadherin-[p-Ser838--p-Ser840]-Antibody-Immunoprecipitation-NBP3-12922-img0003.jpg)
![N/A E-Cadherin [Biotin]](http://images.novusbio.com/fullsize2/ECadherin_DY648_ELISA_2718.jpg "N/A E-Cadherin [Biotin]")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435227.jpg "Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32290) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32290, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with 33258 (blue).](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29820241435238.jpg)
![Immunocytochemistry/ Immunofluorescence: E-Cadherin Antibody (SY0287) [NBP3-32290] - Immunocytochemistry analysis of HT-29 (positive) and MDA-MB-231 (negative) cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202410484863.jpg)
![Flow Cytometry: E-Cadherin Antibody (SY0287) [NBP3-32290] - Flow cytometric analysis of HT-29 (positive, red) and MDA-MB-231 (negative, green) cells labeling E-Cadherin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32290, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HT-29 black, MDA-MB-231 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-29202416453231.jpg)
![Western Blot: E-Cadherin Antibody (SY0287) [NBP3-32290] - Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (NBP3-32290) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: HT-29 cell lysate Lane 4: HCT 116 cell lysate Lane 5: A431 cell lysate Lane 6: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 80~120 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32290_rabbit-e-cadherin-mab-sy0287-3920248453539.jpg)