CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="Integrin a9 was detected in perfusion fixed frozen sections of mouse liver tissue using Goat Anti-Mouse Integrin a9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3827) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB3827P | |||
| Carrier Free | AF3827-SP | |||
HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Human Integrin a9 Monoclonal Antibody (Catalog # MAB4574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4574-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21961-50ul | |||
![<b>Biological Strategies Validation. </b>Intergins alpha 4/ alpha 9 are responsible for the action of osteopontin (OPN) in the augmentation of macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Relative mean fluorescence intensities (MFIs) of integrins alpha 4/ alpha 9 were measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 antibodies (Abs) and each isotype-matched control Ab were used at 10 µg/mL, n = 4 [***p < 0.001 vs. untreated, ###p < 0.001 vs. interleukin (IL)-10 alone]. (B) Representative confocal laser scanning immunofluorescence overlay images of integrin alpha 4 (green) and DAPI (blue), as well as those of integrin alpha 9 (red) and DAPI (blue) in Mφ (–) and Mφ (IL-10 + IL-18). Scale bar represents 20 µm. (C) Relative MFI of CD163 was measured by FACS analysis in each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 4 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset. Anti-integrin alpha 4 or alpha 9 Abs and each isotype-matched control Ab were used at 10 µg/mL, n = 6 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, ###p < 0.001, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). Integrin alpha 4 = ITGA4; Integrin alpha 9 = ITGA9. All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af3827_mouse-integrin-alpha-9-affinity-purified-polyclonal-ab-41202412402515.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 9 Antibody [NBP2-16972] - Cultured human keratinocytes plated on fibronectin coated coverslips. Anti integrin alpha 9 antibody diluted 1:50 in 5% BSA, incubated at room temperature for 3 hours. Nuclei stained with DAPI (green) and f-actin with phalloidin (purple). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Integrin-alpha-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16972-img0003.jpg "Immunocytochemistry/Immunofluorescence: Integrin alpha 9 Antibody [NBP2-16972] - Cultured human keratinocytes plated on fibronectin coated coverslips. Anti integrin alpha 9 antibody diluted 1:50 in 5% BSA, incubated at room temperature for 3 hours. Nuclei stained with DAPI (green) and f-actin with phalloidin (purple). ICC/IF image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Integrin alpha 9 Antibody [NBP2-16972] - Human hepatoma, using Integrin alpha 9 antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Integrin-alpha-9-Antibody-Immunohistochemistry-Paraffin-NBP2-16972-img0002.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin alpha 9 antibody [N1N2], N-term (NBP2-16972) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16972_rabbit-polyclonal-integrin-alpha-9-antibody-western-blot-261120251848230.jpg)
![Western Blot: Integrin alpha 9 Antibody (3E4) [H00003680-M01] - ITGA9 monoclonal antibody (M01), clone 3E4. Analysis of ITGA9 expression in HepG2.](http://images.novusbio.com/fullsize/Integrin-alpha-9-Antibody-3E4-Western-Blot-H00003680-M01-img0001.jpg "Western Blot: Integrin alpha 9 Antibody (3E4) [H00003680-M01] - ITGA9 monoclonal antibody (M01), clone 3E4. Analysis of ITGA9 expression in HepG2.")
![Western Blot: Integrin alpha 9 Antibody (SR1155) [NBP3-21961] - Western blot analysis using NBP3-21961 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21961_rabbit-integrin-alpha-9-mab-sr1155-672023953377.jpg "Western Blot: Integrin alpha 9 Antibody (SR1155) [NBP3-21961] - Western blot analysis using NBP3-21961 in HepG2 cell lysate.")
![SDS-Page: Recombinant Human Integrin alpha 9 Protein [H00003680-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003680-Q01-1.jpg "SDS-Page: Recombinant Human Integrin alpha 9 Protein [H00003680-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Integrin alpha 9 Overexpression Lysate (Adult Normal) [NBL1-12067] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 9.](http://images.novusbio.com/fullsize/Integrin-alpha-9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12067-img0002.jpg "Western Blot: Integrin alpha 9 Overexpression Lysate (Adult Normal) [NBL1-12067] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Integrin alpha 9.")
![Western Blot: Integrin alpha 9 Antibody [NBP3-10039] - Western blot analysis of Integrin alpha 9 in Human Neurofibroma Tumor lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/Integrin-alpha-9-Antibody-Western-Blot-NBP3-10039-img0001.jpg "Western Blot: Integrin alpha 9 Antibody [NBP3-10039] - Western blot analysis of Integrin alpha 9 in Human Neurofibroma Tumor lysates. Antibody dilution at 1ug/ml")
