| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44302SS | |||
| Azide and BSA Free | NBP2-44304SS | |||
| Azide and BSA Free | NBP2-44304 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44300SS | |||
| Azide and BSA Free | NBP2-44301SS | |||
| Azide and BSA Free | NBP2-44301 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34848-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64959-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85325-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09015-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28160 | |||
| FITC | NBP1-28159 | |||
| PE | NBP1-28161 | |||
![Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-M5-114-Azide-Free-Flow-Cytometry-NBP2-21789-img0001.jpg "Flow Cytometry: MHC Class II Antibody (M5/114) - Azide Free [NBP2-21789] - Staining of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC.")
![Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Western-Blot-NBP2-44302-img0005.jpg "Western Blot: HLA DMA Antibody (6B12NB) [NBP2-44302] - Western blot analysis of (A) partial recombinant human HLA DMA protein, and lysates of (B) Ramos cells and (C) Raji cells using 1:500 dilution of HLA DMA antibody (clone 6B12NB)")
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (6B12NB) [NBP2-44302] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (6B12NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Immunocytochemistry-Immunofluorescence-NBP2-44302-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody (6B12NB) [NBP2-44302] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 6B12NB). The antibody generated a punctate staining in the cytoplasm/perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Immunohistochemistry-Paraffin-NBP2-44302-img0003.jpg)
![Flow (Cell Surface): HLA DMA Antibody (6B12NB) [NBP2-44302] - Analysis using the Azide Free version of NBP2-44302. Detection of Human PBMC cells (surface) with HLA DMA (6B12NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-6B12NB-Flow-Cell-Surface-NBP2-44302-img0008.jpg)
![Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Western-Blot-NBP2-44300-img0004.jpg "Western Blot: HLA DMA Antibody (5C9NB) [NBP2-44300] - Western blot analysis of HLA DMA (5C9NB) in A. partial recombinant human HLA DMA protein B. Ramos lysate and C. Raji lysate.")
![Immunocytochemistry/Immunofluorescence: HLA DMA Antibody (5C9NB) [NBP2-44300] - Daudi cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with MHC Class II (5C9NB) at a 1:40 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Actin was detected using Phalloidin 568 (Red). Nuclei were detected using DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Immunocytochemistry-Immunofluorescence-NBP2-44300-img0005.jpg)
![Immunohistochemistry: HLA DMA Antibody (5C9NB) [NBP2-44300] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human tonsil using 1:200 dilution of HLA DMA antibody (clone 5C9NB). The antibody generated a diffused to punctate staining in cellular membranes and cytoplasm, especially in the perinuclear region representing late endosomal localization of major histocompatibility complex, class II, DM alpha/HLA DMA protein.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Immunohistochemistry-NBP2-44300-img0003.jpg)
![Flow (Cell Surface): HLA DMA Antibody (5C9NB) [NBP2-44300] - Analysis using the Azide Free version of NBP2-44300. Detection of Human PBMC cells (surface) with HLA DMA (5C9NB) antibody (red) or isotype control (mouse IgG1; green). Blue line represents cells alone. Positive staining was observed using PE conjugated mouse anti-IgG(H+L) secondary antibody. Live cells were gated (FL-2) for analysis.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-5C9NB-Flow-Cell-Surface-NBP2-44300-img0007.jpg)
![Flow Cytometry: MHC Class II Antibody (CVS20) [NBP2-34848] - MHC Class II expression in human PBMCs. Image from verified customer review.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-CVS20-Flow-Cytometry-NBP2-34848-img0002.jpg "Flow Cytometry: MHC Class II Antibody (CVS20) [NBP2-34848] - MHC Class II expression in human PBMCs. Image from verified customer review.")
![Flow Cytometry: MHC Class II Antibody (CVS20) [NBP2-34848] - Staining of equine peripheral blood lymphocytes](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-CVS20-Flow-Cytometry-NBP2-34848-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice reared in a pathogen free environment for the purposed of comparing with mice reared in standard living conditions. [PMID: 24376530]](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-ER-TR2-Immunocytochemistry-Immunofluorescence-NB100-64959-img0003.jpg "Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice reared in a pathogen free environment for the purposed of comparing with mice reared in standard living conditions. [PMID: 24376530]")
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Comparison of MHC II levels in retinal microglia from mice without transplanted GFP+ BM Cells (panel A) and from mice with transplanted GFP+ BM cells. [PMID: 23750207]](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-ER-TR2-Immunocytochemistry-Immunofluorescence-NB100-64959-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (ER-TR2) [NB100-64959] - Staining of MHC II in conjunctiva associated lymphoid tissue in mice kept in standard living conditions for the purpose of comparing to MHC II expression over time in mice kept in pathogen free environment. [PMID: 24376530]](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-ER-TR2-Immunocytochemistry-Immunofluorescence-NB100-64959-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-7420252226526.png "Immunocytochemistry /Immunofluorescence: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with NBP3-43539 at 10 ug/ml for 1h followed by Alexa Fluor488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining of a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-43539, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or NBP3-43539(B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522141218.png)
![Western Blot: MHC Class II Antibody (OX-6) - Chimeric - BSA Free [NBP3-43539] - Rat spleen (A), rat thymus (B) and rat lymph node (C) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-43539 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD74 is ~24.5-31.6kDa, depending on the isoform and post-translational modification. NBP3-43539 successfully detected CD74 in rat spleen, thymus and lymph node lysates.](http://images.novusbio.com/fullsize/nbp3-43539_rabbit-mhc-class-ii-mab-ox-6-chimeric-azide-bsa-free-74202522113030.png)
![Western Blot: HLA DMA Antibody [NBP1-85325] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DMA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Western-Blot-NBP1-85325-img0009.jpg "Western Blot: HLA DMA Antibody [NBP1-85325] - Analysis in control (vector only transfected HEK293T lysate) and HLA-DMA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining in human tonsil and cerebral cortex tissues using anti-HLA-DMA antibody. Corresponding HLA-DMA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunohistochemistry-Paraffin-NBP1-85325-img0008.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunohistochemistry-Paraffin-NBP1-85325-img0005.jpg)
![Immunohistochemistry-Paraffin: HLA DMA Antibody [NBP1-85325] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-Immunohistochemistry-Paraffin-NBP1-85325-img0006.jpg)
![Western Blot: HLA DMA Antibody (3F12-F11) [H00003108-M01] - HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Analysis of HLA-DMA expression in U-2 OS.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-Western-Blot-H00003108-M01-img0009.jpg "Western Blot: HLA DMA Antibody (3F12-F11) [H00003108-M01] - HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Analysis of HLA-DMA expression in U-2 OS.")
![Immunohistochemistry-Paraffin: HLA DMA Antibody (3F12-F11) [H00003108-M01] - Analysis of monoclonal antibody to HLA-DMA on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-Immunohistochemistry-Paraffin-H00003108-M01-img0007.jpg)
![ELISA: HLA DMA Antibody (3F12-F11) [H00003108-M01] - Detection limit for recombinant GST tagged HLA-DMA is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-ELISA-H00003108-M01-img0004.jpg)
![ELISA: HLA DMA Antibody (3F12-F11) [H00003108-M01] - Detection limit for recombinant GST tagged HLA-DMA is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HLA-DMA-Antibody-3F12-F11-ELISA-H00003108-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09015-img0002.jpg "Immunocytochemistry/Immunofluorescence: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with the chimeric rabbit IgG version of P7/7 (NBP3-09015) at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09015, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: MHC Class II Antibody (P7/7) - Chimeric [NBP3-09015] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of P7/7 (NBP3-09015, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-P7-7-Chimeric-Flow-Cytometry-NBP3-09015-img0001.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-UNLB followed by Mouse Anti-Rat IgG2b-AF488.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0009.jpg "Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse MHC Class II-UNLB followed by Mouse Anti-Rat IgG2b-AF488.")
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the Biotin conjugate of NBP1-28158. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0006.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the FITC conjugate of NBP1-28158. Staining of less or equal to ug/10^6 BALB/c spleen cells with Rat Anti-Mouse MHC II-FITC, following which small lymphocytes were gated and analyzed on a flow cytometer.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0007.jpg)
![Flow Cytometry: MHC Class II Antibody (NIMR-4) [NBP1-28158] - Analysis using the PE conjugate of NBP1-28158. Multiple staining of BALB/c splenocytes.](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-NIMR-4-Flow-Cytometry-NBP1-28158-img0008.jpg)
![Flow Cytometry: MHC Class II Antibody (H42A) [NBP2-61023] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/MHC-Class-II-Antibody-H42A-Flow-Cytometry-NBP2-61023-img0001.jpg "Flow Cytometry: MHC Class II Antibody (H42A) [NBP2-61023] - Obtained with bovine leukocytes")