Progranulin/PGRN: Novus Biologicals

AF2557

Progranulin/PGRN Antibody [Unconjugated]

(1 Review)

100 μg

AF2557

HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>PGRN immunolabeling following ND-602.(A) Representative photomicrographs depicting PGRN immunolabeling in the dentate gyrus of the Tg2576 mouse brain following unilateral intracerebral administration of either LV-GFP, or LV-PGRN (ND-602). (B) The hippocampal density of PGRN immunolabeling was significantly elevated following ND-602 administration in both the ipsilateral and contralateral hemispheres. (C) PGRN protein expression (progranulin/ beta -actin gray values) in the hippocampus was detected by western blot assay. (D) Representative fluorescent photomicrographs depicting PGRN immunolabeling in the entorhinal cortex and frontal cortex following unilateral intracerebral administration of either LV-GFP, or LV-PGRN (ND-602). (E) The density of PGRN immunolabeling was significantly elevated following ND-602 administration in both hemispheres. By contrast, elevations in the (F) frontal cortex did not reach statistical significance. (G) Representative fluorescent photomicrograph depicting GFP (green) and NeuN (red) immunolabeling throughout the ipsilateral hippocampus following lentiviral delivery. The image is a tiled composite of multiple images obtained at 20X magnification. A closer view, (H) shows viral transduction of both NeuN-positive and–negative cells. Each bar represents the mean (± S.E.M.) (n = 8–10) optical density measured across 4 coronal sections. ** sig. diff. from GFP-treated controls, p < 0.001; * p < 0.05. ++ sig. diff. from contralateral hemisphere, p < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28837568), licensed under a CC-BY license. Not internally tested by R&D Systems.

PSAP and PGRN levels are decreased in cingulate cortex tissue of schizophrenia patients. (A) Representative immunoblots of PSAP, PGRN, and beta -actin in cingulate cortex tissue from subjects of healthy control (Ctrl), schizophrenia (SCZ), bipolar disorder (BP), and major depressive disorder (MDD). Upper panel, illustration of cingulate cortex in human brain. (B,C) Bar graphs presenting quantification of the ratio of protein levels of PSAP/ beta -actin (B) and PGRN/ beta -actin (C). (D,E) Scatter plots showing the Pearson’s correlation analysis of PSAP and lifetime quantity of fluphenazine or equivalent (FLU) in schizophrenia (D) and bipolar disorder group (E), respectively. Each dot represents one patient of SCZ (blue) or BP (magenta). (F,G) Scatter plots showing the Pearson’s correlation analysis of PGRN and FLU in schizophrenia (F) and bipolar disorder group (G), respectively. One-way ANOVA with Sidak’s post hoc test. N = 11–12 samples in each group. Data are presented as mean ± S.E.M. * p ˂ 0.05 compared to Ctrl. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36233357), licensed under a CC-BY license. Not internally tested by R&D Systems.

Conditional deletion of neuronal PSAP in the cingulate cortex causes downregulation of neuronal PGRN. (A) Representative fluorescent in situ hybridization images of PSAP mRNA in the cingulate cortex of mice injected with AAV-GFP and AAV-GFP-Cre. Upper row, schematic depiction of stereotaxic injection sites in one hemisphere (the same in the other hemisphere). Scale bar, 50 μm. (B) Representative immunofluorescent staining images of GFP (cyan), Cre (orange), PSAP (magenta), and PGRN (yellow) in the cingulate cortex of mice injected with AAV-GFP and AAV-GFP-Cre, respectively. Scale bar, 20 μm. (C,D) Bar graphs showing quantification of mean fluorescence intensity (MFI) of PSAP (C) and PGRN (D) staining in GFP- or Cre-positive cells. A.U, arbitrary unit. Student’s t-test, N = 34 cells in each group. Data are presented as mean ± S.E.M. **** p ˂ 0.0001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36233357), licensed under a CC-BY license. Not internally tested by R&D Systems.

PSAP ablation does not affect PGRN lysosomal trafficking in BV2 cells. (A) Representative confocal images of BV2 cells stained with sheep anti-PGRN, rat anti-LAMP1 and rabbit anti-PSAP antibodies. Scale bar, 10 µm. (B) Quantification of PGRN signals inside LAMP1-positive vesicles in A. Mean ± SEM; n = 3, Student’s t-test, ns, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35169707), licensed under a CC-BY license. Not internally tested by R&D Systems.

PGRN processing in Sortilin- and PSAP-deficient mice. (A,B) Western blot analysis of PGRN and granulin peptides in the cortical lysates from P21 WT, Sort−/− and Sort−/− Psap−/− mice. The levels of PGRN and granulin peptides (GRNs) were quantified and normalized to GAPDH. Lysates from three mice were analysed for each genotype (n = 3), one-way ANOVA, *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35169707), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2557U-100UG
Alexa Fluor 405	AF2557V-100UG
Alexa Fluor 488	AF2557G-100UG
Alexa Fluor 532	AF2557X-100UG
Alexa Fluor 594	AF2557T-100UG
Alexa Fluor 647	AF2557R-100UG
Alexa Fluor 700	AF2557N-100UG
Alexa Fluor 750	AF2557S-100UG
Alexa Fluor Plus 405	AF2557AFP405-100UG
Alexa Fluor Plus 488	AF2557AFP488-100UG
Alexa Fluor Plus 555	AF2557AFP555-100UG
Alexa Fluor Plus 594	AF2557AFP594-100UG
Alexa Fluor Plus 647	AF2557AFP647-100UG
Alexa Fluor Plus 680	AF2557AFP680-100UG
Allophycocyanin	AF2557A
Biotin	BAF2557
CoraFluor 1	AF2557CL1
DyLight 350	AF2557D
DyLight 405	AF2557E
DyLight 488	AF2557K
DyLight 550	AF2557L
DyLight 594	AF2557M
DyLight 650	AF2557W
DyLight 680	AF2557Y
DyLight 755	AF2557Z
FITC	AF2557F
HRP	AF2557H
Janelia Fluor 525	AF2557JF525
Janelia Fluor 549	AF2557I
Janelia Fluor 646	AF2557J
Janelia Fluor 669	AF2557JF669
PE	AF2557P
PerCP	AF2557C
mFluor Violet 450 SE	AF2557MFV450
mFluor Violet 500 SE	AF2557MFV500
mFluor Violet 610 SE	AF2557MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2557-SP

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AF2420

Progranulin/PGRN Antibody [Unconjugated]

(37 Publications)

100 μg

AF2420

HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Progranulin was detected in paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Progranulin knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2420) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for Progranulin/PGRN at approximately 80 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Genetic Strategies Validation. </b>GRN levels are regulated by SORT1 and TMEM106B expression. A, HAP1 PGRN KO cells were treated with C-TAP PGRN or N-TAP PGRN (5 µg/ml) for 24 h and lysates were analyzed for PGRN and GRN-2,3 by immunoblot. B, HAP1 WT, SORT1 KO, and PGRN KO cell lysates were analyzed for endogenous levels of SORT1, PGRN, and GRN-2,3 by immunoblot. C, D, Quantification of (C) PGRN and (D) GRN-2,3 from the experiment in B. E, Overexpression of TMEM106B in HeLa cells for 48-h results in the formation of large vacuoles. Scale bar, 20 µm. F, HeLa cells were transfected with empty vector or TMEM106B for 48 h and lysates were analyzed for PGRN and GRN-2,3 by immunoblot. G-I, Quantification of (G) intracellular PGRN, (H) intracellular GRN-2,3, and (I) secreted PGRN (by ELISA) from the experiment in F. Arrow in F denotes endogenous, intermediate PGRN cleavage product. J, Overexpression of TMEM106B for 24 h in HAP1 PGRN KO cells results in the formation of large vacuoles. Scale bar, 10 µm. K, HAP1 PGRN KO cells were transfected with TMEM106B for 24 h and then treated with mCherry-PGRN (5 µg/ml) for an additional 24 h. Lysates were analyzed for PGRN and GRN-2,3 by immunoblot. L, M, Quantification of (L) PGRN and (M) GRN-2,3 from the experiment in K. For all immunoblots, PGRN and GRN-2,3 were detected with R&D AF2420 and Sigma antibodies, respectively. All immunoblot images are representative of at least three independent experiments and quantitative data are presented as mean ± SEM of three independent experiments; *differs from control p < 0.05; **p < 0.01; ***p < 0.001; ns = not significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28828399), licensed under a CC-BY license. Not internally tested by R&D Systems.

Progranulin Interactions with AD pathological Features. (a-c). Representative photomicrographs of progranulin (PGRN)(purple) immunoreactivity associated with amyloid beta (A beta ) plaques (brown) in MTG sections of low plaque, high plaque and Alzheimer’s disease cases. Scale bar represents 30 μm. (d-f). Photomicrographs of PGRN (purple) immunoreactivity associated with CD45 immunoreactive microglia in MTG sections of low plaque (d), high plaque (e), and Alzheimer’s disease cases (f). Insets a) show at higher magnification PGRN-positive stained neurons present in each section. Neurons are identified by their size and characteristic shape. Insets b) show higher magnification of PGRN-positive microglia. Scale bar represents 20 μm (d-f), and 10 μm for insets. (g-i). Photomicrographs of PGRN (purple) with plaque-associated CD45-positive microglia (brown). Progressive increase in accumulation of CD45-positive microglia in low plaque (g), high plaque (h) and Alzheimer’s disease (i) cases. Scale bar represents 30 μm. (j-o). Absence of PGRN immunoreactivity of neurofibrillary tangles. (j-i) Photomicrographs of PGRN (purple) and phosphorylated tau (AT8)(brown) double-stained sections from low plaque (j), high-plaque (k), and Alzheimer’s disease cases (l). (m-o). Confocal micrographs of PGRN (green) and phosphorylated tau (AT180)-positive tangles in low-plaque (m), high-plaque (n) and Alzheimer’s disease (o) cases. Scale bar represents 10 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31864418), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Hypoglycosylation of proteins in knockout HEK293 cell lines.Cell lines were transfected with expression vectors for the following human glycoproteins: (a) SHBG, (b) SHBG derivatives (see diagram) with single glycosylation sites (SHBG N380Q and N396Q), (c) a cathepsin C derivative with a single glycosylation site (CatC delta 234-HA), (d) prosaposin (pSAP-DDKHis), (e) haptoglobin (Hp-DDKHis), (f) hemopexin (Hpx-DDKHis). Endogenous progranulin (pGran) (d) was analyzed using non-transfected cells. The cells were pulse labeled for 10 min and chased for 10 min (pSAP, pGran and CatC delta 234-HA) or pulse labeled for 5 min and chased for 20 min (Hp, SHBG and Hpx). Glycoproteins precipitated with anti-DDK, anti-HA or anti-SHBG were resolved by PAGE in SDS. EH designates treatment with endoglycosidase H. Quantified values below gel lanes (a–f) are for the displayed image, which is representative of two or more experiments. (b) The vertical line indicates the excision of three intervening gel lanes. (d) Resolution of pGran glycoforms is not sufficient for quantification. Nonglycosylated forms of pSAP, Hp, and Hpx that comigrate with the EH-digested form of the substrate in HEK293 cells correspond to a non-translocated precursor and were not used to calculate the average number of glycans. (a–f) Phosphorimages were cropped to display the region of interest. Full-length phosphorimages are shown in Supplemental Fig. S5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26864433), licensed under a CC-BY license. Not internally tested by R&D Systems.

Confocal microscopy of prosaposin localization on plaques and different cell types. (a-c). A beta (green) (a) and PSAP (red) plaque (b) with limited colocalization (C – yellow) in an AD case. Scale bar represents 30 μm. (d-f). Comparison of colocalization in plaque of PGRN (green) and A beta (blue) (d) with PSAP (red) and A beta (blue) in triple-stained AD section. Merged images show extensive colocalization of PGRN and PSAP (yellow) but limited overlap with A beta -positive structures. Scale bar represents 30 μm. (G-I). Merged images of PSAP (red) immunoreactivity with microglial markers IBA-1 (g) and CD68 (h) (green) and astrocyte marker GFAP (green) show some expression of PSAP in both cell types (yellow). These images show that PSAP (red) is predominantly in cells with morphology of neurons. Scale bar represents 10 μm. (j-l). Merged images of CD68 (green) and PSAP (red) on plaques in low plaque case (J), high plaque case (k) and AD case (l). Significant amounts of PSAP immunoreactivity (red) can be observed on all plaques but with only limited colocalization with CD68 in infiltrating microglia. Scale bar represents 30 μm. (m-n) Merged images of AT180 (pTau) (green) and PSAP (red) on tangle in low plaque case (M), high plaque case (n) and Alzheimer’s disease case (o). Very limited amounts of PSAP immunoreactivity (yellow) can be observed on tangles. Panel M and N show intracellular tangles with DAPI-positive nuclei, while panel O shows extracellular tangle. Scale bar represents 30 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31864418), licensed under a CC-BY license. Not internally tested by R&D Systems.

Biochemical analysis of interactions of progranulin and prosaposin in MTG brain samples. (a-b). Co-immunoprecipitation of Progranulin and Prosaposin. a). Western blot control analyses to show interactions of progranulin (PGRN) and prosaposin (PSAP). Immunoprecipitation of PGRN-overexpressing HEK (HEK and HEK1) cells and AD brain sample with protein G- (goat antibody) or protein A- (rabbit antibody) antibody-conjugated magnetic beads. Beads were prepared using non-immune goat IgG, goat anti-PGRN and rabbit anti-PSAP. Immunoprecipitated samples were separated by gel electrophoresis, transferred to membranes, and probed with antibodies to PSAP and PGRN. Samples of total protein (non-immunoprecipitated) from PGRN-overexpressing HEK (HEK) cells and brain sample (AD) were analyzed as specificity controls. Samples immunoprecipitated with PGRN antibody contained PSAP, and samples precipitated with PSAP antibody contained PGRN. b). All of the brain samples from LP, AD and HP cases precipitated with PGRN antibody pulled-down PSAP. A series of cases (n = 5) from each group were analyzed. Western blot images for both antibodies are shown. (c-f) Biochemical analysis of prosaposin in MTG brain protein extracts. c). Western blot analysis of total PSAP protein levels in MTG samples. Representative image of western blot demonstrating PSAP protein in samples from LP, HP and AD cases. The complete images of all samples analyzed for PSAP are shown as Additional file 4: Figure. S4B. D). Scatter plot showing expression levels of PSAP protein in the different groups. Results represent mean ±S.E.M. Significantly increased levels of PSAP protein were present in AD case compared to LP and HP cases but not between LP and HP cases. Significant increased levels of PSAP protein in MTG samples from AD compared to HP (* p < 0.05) and LP (** p < 0.01). e). Positive correlation between PSAP and PGRN protein levels in MTG samples (Pearson r = 0.4674, p = 0.0070). f). Positive correlation between PSAP

Biochemical analyses of Progranulin in MTG samples compared to A beta and phosphorylated tau. a). Scatter plot showing expression levels of PGRN protein in the different groups. Results presented represent the combination of levels of 75–80 kDa and lower molecular weight (55 kDa) PGRN protein bands. Results represent mean ±S.E.M. Significantly increased levels of PGRN protein were present in AD case compared to LP and HP cases but not between LP and HP cases (** p < 0.01, ns – not significant). b). Scatter plot showing expression levels of A beta protein in the different groups. Results presented represent the levels of all p-tau detected bands. Results represent mean ±S.E.M. Significantly increased levels of A beta (monomer) protein were present in AD case compared to LP and AD cases but not between LP and HP or HP and AD cases (** p < 0.01, ns – not significant). Outlier HP case indicated by red arrow (see text). c). Scatter plot showing expression levels of phosphorylated tau (p-tau – Thr 231) protein in the different disease groups. Results presented represent the levels of all p-tau detected bands. Results represent mean ±S.E.M. Significantly increased levels of p-tau protein were present in AD cases compared to LP and HP cases but not between LP and HP cases (* p < 0.05, ** p < 0.01, ns – not significant). d). Western blots showing bands detected with goat antibody to PGRN (PGRN) in MTG protein extracts from low plaque (LP), high plaque (HP) and AD cases. The complete series of samples are presented in Additional file 4: Fig. S4A). The same blots were reprobed with antibody AT180 to phosphorylated tau (pTau), and beta -actin for normalization purposes. e). Western blots showing bands detected with antibody 6E10 to A beta in MTG protein extracts from low plaque (LP), high plaque (HP) and AD cases. Results represent mean ±S.E.M. Samples were separate from those in panel A by using Tris-tricine gels to resolve low molecular weight bands. f). Correlation Analyses between PGRN prote

<b>Genetic Strategies Validation. </b>PGRN is an endogenous anti-HIV factor.Microglial PGRN was knocked down using RNAi 2–4 days prior to VSVg env HIV exposure as described in the Materials and Methods. Control cultures were treated with control, irrelevant siRNA (Ctr). The amounts of HIV (p24) and PGRN expression were determined by western blot analyses. (A) A representative western blot showing suppression of PGRN and increase of p24 following PGRN siRNA treatment. (B) Pooled densitometry data from four independent experiments showing significant inhibition of PGRN and increase of HIV (gag p24 express) in microglial cells treated with PGRN siRNA (vs. control siRNA). **P<0.01, ***P<0.001 by paired t-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24878635), licensed under a CC-BY license. Not internally tested by R&D Systems.

Crosslink between progranulin and IL-6 expression in breast cancer cell lines. Immunoblotting of IL-6, IL-8 and progranulin protein expression in MCF7 and MDA-MB-231 breast cancer cell lines. (a) Cells treated with increasing concentrations of progranulin showed an increase in IL-6 and IL-8 expression for 48 h. Treatment with an IL-6 (b) or IL-8 (c) concentration gradient led to an increased progranulin expression in MCF7 and MDA-MB-231 cells. SYPRO™ Ruby (Bio-Rad) stain was used as a loading control. Representative images from one of three independent experiments are shown. Western blot data are cut for visualization purposes, and all samples for the respective antibody were analyzed on the same blot. PGRN: progranulin, IL: interleukin. The uncropped bolts are shown in Supplementary Materials. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2072-6694/15/24/5757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2420U-100UG
Alexa Fluor 405	AF2420V-100UG
Alexa Fluor 488	AF2420G-100UG
Alexa Fluor 532	AF2420X-100UG
Alexa Fluor 594	AF2420T-100UG
Alexa Fluor 647	AF2420R-100UG
Alexa Fluor 700	AF2420N-100UG
Alexa Fluor 750	AF2420S-100UG
Alexa Fluor Plus 405	AF2420AFP405-100UG
Alexa Fluor Plus 488	AF2420AFP488-100UG
Alexa Fluor Plus 555	AF2420AFP555-100UG
Alexa Fluor Plus 594	AF2420AFP594-100UG
Alexa Fluor Plus 647	AF2420AFP647-100UG
Alexa Fluor Plus 680	AF2420AFP680-100UG
Allophycocyanin	AF2420A
Biotin	BAF2420
CoraFluor 1	AF2420CL1
DyLight 350	AF2420D
DyLight 405	AF2420E
DyLight 488	AF2420K
DyLight 550	AF2420L
DyLight 594	AF2420M
DyLight 650	AF2420W
DyLight 680	AF2420Y
DyLight 755	AF2420Z
FITC	AF2420F
HRP	AF2420H
Janelia Fluor 525	AF2420JF525
Janelia Fluor 549	AF2420I
Janelia Fluor 646	AF2420J
Janelia Fluor 669	AF2420JF669
PE	AF2420P
PerCP	AF2420C
mFluor Violet 450 SE	AF2420MFV450
mFluor Violet 500 SE	AF2420MFV500
mFluor Violet 610 SE	AF2420MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2420-SP

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DPGRN0

Human Progranulin Quantikine ELISA Kit

(20 Publications)

1 Kit

DPGRN0

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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2420-PG

Recombinant Human Progranulin Protein, CF

(20 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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2557-PG

Recombinant Mouse Progranulin Protein, CF

(14 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB2420

Progranulin/PGRN Antibody (296628) [Unconjugated]

(7 Publications)

100 μg

MAB2420

Specificity of PGRN antibodies and detection of multiple endogenous GRNs. A, Whole-cell lysates from HAP1 WT and HAP1 PGRN KO cells, either reduced (+TCEP) or nonreduced (-TCEP), were analyzed for PGRN antibody specificity by immunoblot. The bottoms of the blots (<15 kDa) were exposed longer to reveal levels of endogenous GRNs. B, Whole-cell lysates from HEK293T WT and N-TAP PGRN stable overexpressing (PGRN) cells were analyzed as in A by immunoblot. C, Representative immunofluorescent images of HAP1 PGRN KO and WT cells stained with the corresponding PGRN antibodies as in A and B. Scale bar, 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28828399), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2420U-100UG
Alexa Fluor 405	FAB2420V-100UG
Alexa Fluor 488	FAB2420G-100UG
Alexa Fluor 532	FAB2420X-100UG
Alexa Fluor 594	FAB2420T-100UG
Alexa Fluor 647	FAB2420R-100UG
Alexa Fluor 700	FAB2420N-100UG
Alexa Fluor 750	FAB2420S-100UG
Alexa Fluor Plus 405	FAB2420AFP405-100UG
Alexa Fluor Plus 488	FAB2420AFP488-100UG
Alexa Fluor Plus 555	FAB2420AFP555-100UG
Alexa Fluor Plus 594	FAB2420AFP594-100UG
Alexa Fluor Plus 647	FAB2420AFP647-100UG
Alexa Fluor Plus 680	FAB2420AFP680-100UG
Allophycocyanin	FAB2420A
Biotin	FAB2420B
CoraFluor 1	FAB2420CL1
DyLight 350	FAB2420D
DyLight 405	FAB2420E
DyLight 488	FAB2420K
DyLight 550	FAB2420L
DyLight 594	FAB2420M
DyLight 650	FAB2420W
DyLight 680	FAB2420Y
DyLight 755	FAB2420Z
FITC	FAB2420F
HRP	FAB2420H
Janelia Fluor 525	FAB2420JF525
Janelia Fluor 549	FAB2420I
Janelia Fluor 646	FAB2420J
Janelia Fluor 669	FAB2420JF669
PE	FAB2420P
PerCP	FAB2420C
mFluor Violet 450 SE	FAB2420MFV450
mFluor Violet 500 SE	FAB2420MFV500
mFluor Violet 610 SE	FAB2420MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2420-SP

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NBP1-87324

Granulin Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-87324

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human placenta shows moderate to strong granular cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87324] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in striated muscle fibers.

Staining of human cell line A-431 shows localization to endoplasmic reticulum & lysosomes.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87324-25ul

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NBP1-32076

Granulin Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-32076

HepG2 conditioned medium (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Granulins antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

HBL435 xenograft.Granulins antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>HepG2 conditioned medium (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Granulin antibody (NBP1-32076) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br><br>*The competitor is not affiliated withand does not endorse this product.

Deletion of progranulin suppressed apoptosis in the airways exposed to ovalbumin, but aggravated the human bronchial epithelial cells apoptosis in vitro. (A) Representative images of lung sections from WT and PGRN KO mice stained for TUNEL. Scale bar: 100 μm. (B) The protein levels of cleaved caspase 3 in mice lung were analyzed by Western blot. The results of densitometric analysis were showed as means +/- SD (n = 3). *p < .05, unpaired t test. (C) Western blot analysis of PGRN and cleaved caspase 3 in 16‐HBE cells treated (or not) with H2O2 for 48 h. The results of densitometric analysis were showed as means +/- SD (n = 3). ****p < .0001, one‐way ANOVA. (D) The knockdown efficiency of siRNA targeting PGRN was identified at the mRNA and protein levels 24 and 48 h following transfection, respectively. The relative mRNA expression and densitometric analysis were showed as means +/- SD (n = 3). ****p < .0001, one‐way ANOVA. (E) Following knockdown of PGRN for 24 h, 16‐HBE cells were treated (or not) with H2O2 for 24 h. The densitometric analysis of cleaved caspase 3 was presented as means +/- SD (n = 3). *p < .05, unpaired t test. The study was repeated for three times. ANOVA, analysis of variance; DAPI, 4′,6‐diamidino‐2‐phenylindole; HBE, human bronchial epithelial cells; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36840485), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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DY2420

Human Progranulin DuoSet ELISA, 15 Plate

(8 Publications)

1 Kit (for 15 Plates)

DY2420

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NBP2-82200

Rat Granulin ELISA Kit (Colorimetric)

1 Kit

NBP2-82200

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Standard Curve:

0.31 - 20 ng/mL

Sensitivity:

0.19 ng/mL

Sample Type:

Serum, Plasma, Cell supernatant

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MAB25571

Progranulin/PGRN Antibody (333731)

(4 Publications)

100 μg

MAB25571

CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Progranulin was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Progranulin Monoclonal Antibody (Catalog # MAB25571) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Progranulin was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Progranulin Monoclonal Antibody (Catalog # MAB25571) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB25571U-100UG
Alexa Fluor 405	FAB25571V-100UG
Alexa Fluor 488	FAB25571G-100UG
Alexa Fluor 532	FAB25571X-100UG
Alexa Fluor 594	FAB25571T-100UG
Alexa Fluor 647	FAB25571R-100UG
Alexa Fluor 700	FAB25571N-100UG
Alexa Fluor 750	FAB25571S-100UG
Alexa Fluor Plus 405	FAB25571AFP405-100UG
Alexa Fluor Plus 488	FAB25571AFP488-100UG
Alexa Fluor Plus 555	FAB25571AFP555-100UG
Alexa Fluor Plus 594	FAB25571AFP594-100UG
Alexa Fluor Plus 647	FAB25571AFP647-100UG
Alexa Fluor Plus 680	FAB25571AFP680-100UG
Allophycocyanin	FAB25571A
Biotin	FAB25571B
CoraFluor 1	FAB25571CL1
DyLight 350	FAB25571D
DyLight 405	FAB25571E
DyLight 488	FAB25571K
DyLight 550	FAB25571L
DyLight 594	FAB25571M
DyLight 650	FAB25571W
DyLight 680	FAB25571Y
DyLight 755	FAB25571Z
FITC	FAB25571F
HRP	FAB25571H
Janelia Fluor 525	FAB25571JF525
Janelia Fluor 549	FAB25571I
Janelia Fluor 646	FAB25571J
Janelia Fluor 669	FAB25571JF669
PE	FAB25571P
PerCP	FAB25571C
mFluor Violet 450 SE	FAB25571MFV450
mFluor Violet 500 SE	FAB25571MFV500
mFluor Violet 610 SE	FAB25571MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB25571-SP

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MPGRN0

Mouse Progranulin Quantikine ELISA Kit

(4 Publications)

1 Kit

MPGRN0

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 2896

Progranulin/PGRN Antibody [Unconjugated]

Progranulin/PGRN Antibody [Unconjugated]

Human Progranulin Quantikine ELISA Kit

Recombinant Human Progranulin Protein, CF

Recombinant Mouse Progranulin Protein, CF

Progranulin/PGRN Antibody (296628) [Unconjugated]

Granulin Antibody - BSA Free

Granulin Antibody - BSA Free

Human Progranulin DuoSet ELISA, 15 Plate

Rat Granulin ELISA Kit (Colorimetric)

Progranulin/PGRN Antibody (333731)

Mouse Progranulin Quantikine ELISA Kit