| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44044 | |||
| BSA Free | NBP2-61425-25ul | |||
HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420is recommended." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Progranulin/PGRN Polyclonal Antibody (Catalog # MAB24201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420is recommended." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB24201-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2557-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70847 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87325-25ul | |||
DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Progranulin/PGRN protein was serially diluted 2-fold and captured by Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2557R-SP | |||
MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Mouse Progranulin/PGRN protein was serially diluted 2-fold and captured by Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB2557R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Progranulin/PGRN Monoclonal Antibody (Catalog # MAB25572) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB25572-SP | |||
DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Progranulin/PGRN protein was serially diluted 2-fold and captured by Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB24203-SP | |||
MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Progranulin/PGRN protein was serially diluted 2-fold and captured by Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24203) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Progranulin/PGRN Monoclonal Antibody (Catalog # MAB24202) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB24202-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44043 | |||
| BSA Free | NBP2-61424-25ul | |||
![Western Blot: Granulin Antibody (CL5695) [NBP2-61425] - Analysis in human cell line PC-3.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Western-Blot-NBP2-61425-img0007.jpg "Western Blot: Granulin Antibody (CL5695) [NBP2-61425] - Analysis in human cell line PC-3.")
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0004.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human endometrium shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0001.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human liver shows cytoplasmic immunoreactivity in Kupffer cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5695) [NBP2-61425] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5695-Immunohistochemistry-Paraffin-NBP2-61425-img0003.jpg)
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution.<br/><br/> Lane 1: HepG2 cell lysate<br/> Lane 2: HEK-293 cell lysate<br/> Lane 3: TF-1 cell lysate<br/> Lane 4: THP-1 cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 64 kDa<br/> Observed band size: 50-75 kDa<br/><br/> Exposure time: 1 minutes; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution.<br/><br/> Lane 1: HepG2 cell lysate<br/> Lane 2: HEK-293 cell lysate<br/> Lane 3: TF-1 cell lysate<br/> Lane 4: THP-1 cell lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 64 kDa<br/> Observed band size: 50-75 kDa<br/><br/> Exposure time: 1 minutes; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution.<br/><br/> Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for ![N/A Progranulin/PGRN [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Progranulin_DY2557_ELISA_1845.jpg "N/A Progranulin/PGRN [Biotin]")
![Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Western-Blot-NBP2-45915-img0001.jpg "Western Blot: Granulin Antibody (OTI3H6) [NBP2-45915] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.")
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human liver tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0003.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (OTI3H6) [NBP2-45915] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GRN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Granulin-Antibody-OTI3H6-Immunohistochemistry-Paraffin-NBP2-45915-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87325] - Staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87325-img0004.jpg "Immunocytochemistry/Immunofluorescence: Granulin Antibody [NBP1-87325] - Staining of human cell line A-431 shows localization to endoplasmic reticulum, endosomes & lysosomes. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Granulin Antibody [NBP1-87325] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/Granulin-Antibody-Immunohistochemistry-Paraffin-NBP1-87325-img0005.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0004.jpg "Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).")
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human prostate shows moderate granular cytoplasmic immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0001.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human kidney shows cytoplasmic immunoreactivity in both renal glomeruli and tubules.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0002.jpg)
![Immunohistochemistry-Paraffin: Granulin Antibody (CL5692) [NBP2-61424] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Granulin-Antibody-CL5692-Immunohistochemistry-Paraffin-NBP2-61424-img0003.jpg)
