Alkaline Phosphatase/ALPL: Novus Biologicals

AF2910

Alkaline Phosphatase/ALPL Antibody [Unconjugated]

100 μg

AF2910

HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox">

Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue).<EM>Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA.</EM>View our protocol for <a class=NoLineLink href=

Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Downregulation of HDAC9 rescued lineage differentiation imbalance and ameliorated senescence in aged BMMSCs. a Alizarin Red staining was performed, and osteogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. b Oil Red O staining was performed, and adipogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. c Alizarin Red staining was performed, and osteogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. d Oil Red O staining was performed, and adipogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. e, f Expressions of the senescence-related proteins p53 and p-p53 in BMMSCs cultured in vitro from aged mice (e) and young mice (f) were examined by western blotting. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of HDAC9 improved the lineage differentiation of endogenous BMMSCs ex vivo. The BMMSCs harvested from the mice from the control, shScr-treated, & shHDAC9-treated groups 4 weeks after bone intrainjection. e Alizarin Red staining was performed, & osteogenesis-related proteins detected in BMMSCs from the three group. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

The representative images of Ki‐67 staining (A) and quantified by the positive‐stained percentage through ImageJ software (B). Scale bar, 100 μm. (C‐D) Alizarin Red staining was performed to detect mineralized nodules formed in Con, AS‐Exo and NS‐Exo 14 days after osteogenic induction and quantified with a spectrophotometer after dissolving with cetylpyridinium chloride. (E‐F) Lipid droplet formation was detected by Oil Red O staining 7 days after adipogenic induction with positive area quantified. Scale bar, 100 μm. (G‐H) The protein expression levels of Runx2, ALP and PPAR‐ gamma in Con, AS‐Exo and NS‐Exo groups were measured through Western blot and quantified by ImageJ software. n = 3 per group. Data are shown as mean ± SD; ns, not significant; *P < .05; **P < .01; ***P < .001. AS‐Exo, adult serum exosomes; Con, control; NS‐Exo, neonatal serum exosomes Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32608556), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of autophagy blocked the ability of HDAC9 siRNA to rebalance BMMSC differentiation. To investigate the HDAC9-autophagy axis regulating BMMSC function, BMMSCs were respectively transfected with Nc siRNA and HDAC9 siRNA or co-transfected with HDAC9 siRNA and BECN1 siRNA. a Alizarin Red staining was performed, and osteogenesis-related proteins were analyzed in aged BMMSCs from above three groups. b Oil Red O staining was performed, and adipogenesis-related proteins were analyzed in three groups of cells described above. Scale bars = 100 μm. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, one-way analysis of variance (ANOVA) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32620134), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2910U-100UG
Alexa Fluor 405	AF2910V-100UG
Alexa Fluor 488	AF2910G-100UG
Alexa Fluor 532	AF2910X-100UG
Alexa Fluor 594	AF2910T-100UG
Alexa Fluor 647	AF2910R-100UG
Alexa Fluor 700	AF2910N-100UG
Alexa Fluor 750	AF2910S-100UG
Alexa Fluor Plus 405	AF2910AFP405-100UG
Alexa Fluor Plus 488	AF2910AFP488-100UG
Alexa Fluor Plus 555	AF2910AFP555-100UG
Alexa Fluor Plus 594	AF2910AFP594-100UG
Alexa Fluor Plus 647	AF2910AFP647-100UG
Alexa Fluor Plus 680	AF2910AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2910-SP

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MAB1448

Alkaline Phosphatase/ALPL Antibody (B4-78) [Unconjugated]

(1 Review)

(15 Publications)

100 μg

MAB1448

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." class="big_lightbox">

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." alt="BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B)." class="big_thumb" />

AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Alkaline Phosphatase/ALPL and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (<a class=

AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

Whole blood granulocytes were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Immunocytochemistry of pluripotency markers.Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc immortalized lines (hNS1, hVM1, and hCTX) and fibroblasts (hFF-1). From top to bottom the markers studied were: NANOG, OCT3/4, SOX2 and AP. Hoechst 33258 nuclear staining is shown in blue. Scale bar represents 100 μm for hESCs in the left hand column (low magnification) and 25 μm for all the other microphotographs. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0118499), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1448U-100UG
Alexa Fluor 405	FAB1448V-100UG
Alexa Fluor 488	FAB1448G-100UG
Alexa Fluor 594	FAB1448T-100UG
Alexa Fluor 647	FAB1448R-100UG
Alexa Fluor 700	FAB1448N-100UG
Alexa Fluor 750	FAB1448S-100UG
Alexa Fluor Plus 405	FAB1448AFP405-100UG
Alexa Fluor Plus 488	FAB1448AFP488-100UG
Alexa Fluor Plus 555	FAB1448AFP555-100UG
Alexa Fluor Plus 594	FAB1448AFP594-100UG
Alexa Fluor Plus 647	FAB1448AFP647-100UG
Alexa Fluor Plus 680	FAB1448AFP680-100UG
Allophycocyanin	FAB1448A-025
Allophycocyanin	FAB1448A-100
Biotin	BAM1448
Phycoerythrin	FAB1448P-025
Phycoerythrin	FAB1448P-100

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1448-SP
Carrier Free	FAB1448P-100
Carrier Free	FAB1448P-025
Carrier Free	FAB1448A-100
Carrier Free	FAB1448A-025
Carrier Free	FAB1448V-100UG
Carrier Free	FAB1448U-100UG
Carrier Free	FAB1448T-100UG
Carrier Free	FAB1448S-100UG
Carrier Free	FAB1448R-100UG
Carrier Free	FAB1448N-100UG
Carrier Free	FAB1448G-100UG

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NBP2-67295

Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00)

(10 Publications)

100 ul

NBP2-67295

Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB29092

Alkaline Phosphatase/ALPL Antibody (928929) [Unconjugated]

(5 Publications)

100 μg

MAB29092

HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, BG01V human embryonic stem cells, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Alkaline Phosphatase/ALPL at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to bile canaliculi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, Saos‑2 human osteosarcoma cells and BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for Alkaline Phosphatase/ALPL at approximately 115 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29092) . This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Analysis of the control and CAVD aortic valve samples (n = 5 and 5, respectively). (A) Western blotting for CDK1 and ALP proteins on valve tissues. (B) Semi-quantitative analysis of protein expression. (C) Immunofluorescence staining of CDK1 and Ki-67 on valve tissues. (D) Semi-quantitative analysis of fluorescence intensity. (E) PCR test for cell proliferation genes on valve tissues. (*) p < 0.05 indicates a significant difference. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35571082), licensed under a CC-BY license. Not internally tested by R&D Systems.

CCND1 participates in the osteogenic differentiation of VICs following OM. A–F) VICs were transfected with CCND1 siRNA or scrambled siRNA, and then stimulated with OM for 7 days. Immunoblot analysis of CCND1, ALP, Runx2 P53, and P21 expression in VICs from indicated groups (n = 3, each group). Bar plots showing the semiquantitative analysis of indicated genes expression. G,H) With OM induction for 7 days, representative ALP staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. I–K) With OM induction for 21 days, representative Alizarin red staining showed the calcific nodules in VICs from indicated groups (n = 3, each group). Scale bar 50 µm. L–M) Representative SA‐ beta ‐gal staining of VICs from indicated groups (n = 3, each group). Bar plot showing the percentage of SA‐ beta ‐gal staining positive cells. Scale bar 50 µm. Data are means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Morusin attenuates VIC calcification by activating Nrf2 signaling pathway. A–D) ML385 was used to inhibit the activation of Nrf2 in VICs. Representative immunoblot images and quantification of the levels of ALP, Runx2, and P21 in VICs from indicated groups (n = 3, each group). E,F) With OM induction for 7 days, representative ALP staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. G–I) With OM induction for 21 days, representative Alizarin red staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. Data are means ± SD. **p < 0.01; ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Morusin inhibits OM‐induced osteogenic differentiation of VICs. A) Immunoblot analysis of Runx2 and ALP expression in VICs from indicated groups (n = 3, each group). B) Bar plot showing the fold change of Runx2 expression over control. C) Bar plot showing the fold change of ALP expression over control. D) Immunofluorescent staining of ALP (green), Runx2 (red), and DAPI (blue) in the VICs from indicated groups. Scale bar 50 µm. E,F) With OM induction for 7 days, representative ALP staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. G–I) With OM induction for 21 days, representative Alizarin red staining showed the calcific nodules in VICs from indicated groups (n = 3, each group). Scale bar 50 µm. J) With OM induction for 21 days, representative Von Kossa and Alizarin Red staining of aortic valve leaflets. K) Bar plot showing the percentage of Von Kossa positive staining area of indicated groups (n = 3, each group). L) Bar plot showing the percentage of Alizarin Red positive staining area of indicated groups (n = 3, each group). Data are mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Morusin attenuates VIC calcification depending on Trim25. A,B) VICs were transfected with CCND1 siRNA or scrambled siRNA, immunoblot analysis of Trim25 expression in VICs from indicated groups (n = 3, each group). Bar plots showing the semiquantitative analysis of Trim25 expression. C) VICs were transfected with Trim25 siRNA or scrambled siRNA. Co‐IP analysis of the Keap1 ubiquitination level in VICs from indicated groups (n = 3, each group). D–M) Representative immunoblot images and quantification of the levels of Trim25, ALP, Runx2, and P21 in VICs from indicated groups (n = 3, each group). N,S) VICs were transfected with Trim25 siRNA or scrambled siRNA, representative ALP staining of VICs from indicated groups (n = 3, each group). P,U) VICs were transfected with Trim25 siRNA or scrambled siRNA, representative Alizarin red staining of VICs from indicated groups (n = 3, each group). Scale bar 50 µm. Data are means ± SD. NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA with Tukey's multiple comparisons test). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38502885), licensed under a CC-BY license. Not internally tested by R&D Systems.

Analyses of TNAP expression and activity by immunofluorescence staining, Western blot, and enzyme activity assay. Cell analyses were performed in five different ALPLtg PDL-hTERT cell lines, the wt line and control PDL-hTERT that did not undergo genome editing. (A) TNAP expression (green) was detected by immunofluorescence after nuclear counterstaining with DAPI (blue). Representative images are shown. Scale bars = 50 µm. (B) Quantification of TNAP signals in IF images were done by corrected total cell fluorescence (CTCF) for ten random cells per image, and five images per cell line (n = 50). (C) Specific TNAP activity was measured by a CSPD assay confirming the spontaneous activity of the expressed protein. N = 4 per condition. (D–F) TNAP expression level and the expression of the mitochondrial protein TOMM20 in total cell lysates were determined semi-quantitatively by Western blot analysis in relation to the housekeeping protein beta -actin/ACTB. A lysate of a TNAP overexpressing cell line (ALPL over) was used as positive control. A representative immunoblot is shown in (C). White pixels indicate signal oversaturation. Quantification results in E and F are presented as mean ± SEM, N = 3 per condition. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40530336), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB29092U-100UG
Alexa Fluor 405	FAB29092V-100UG
Alexa Fluor 488	FAB29092G-100UG
Alexa Fluor 532	FAB29092X-100UG
Alexa Fluor 594	FAB29092T-100UG
Alexa Fluor 647	FAB29092R-100UG
Alexa Fluor 700	FAB29092N-100UG
Alexa Fluor 750	FAB29092S-100UG
Alexa Fluor Plus 405	FAB29092AFP405-100UG
Alexa Fluor Plus 488	FAB29092AFP488-100UG
Alexa Fluor Plus 555	FAB29092AFP555-100UG
Alexa Fluor Plus 594	FAB29092AFP594-100UG
Alexa Fluor Plus 647	FAB29092AFP647-100UG
Alexa Fluor Plus 680	FAB29092AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB29092-SP

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NB600-540

Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free

(4 Publications)

0.05 mg

NB600-540

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).

Comparison on osteogenic-related proteins (OCN, BMP2, COL1a1, DDk1, ALP) for osteogenic micro-tissue and digested cells by Western blot. MT, osteogenic micro-tissue; DC, digested cells

Reactivity:

Hu, Po, Fe, Rb

Species Glossary

Reactivity: Hu, Po, Fe, Rb

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-68197

Human Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric)

1 Kit

NBP2-68197

Human Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) - Standard Curve Reference

ELISA: Human Alkaline Phosphatase, Liver/Bone/Kidney ELISA Kit (Colorimetric) [NBP2-68197] - Standard Curve Reference

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

78.13 - 5000 pg/mL

Sensitivity:

46.88 pg/mL

Sample Type:

Serum, Plasma, Saliva, Urine, Milk, Cell Supernatant, Tissue Homogenate

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NBP2-22193

Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free

(2 Publications)

0.1 ml

NBP2-22193

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Image from verified customer reviews.

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mAb against human Alkaline Phosphatase, Tissue Non-Specific (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)

Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.

Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of MCF-7 cells using Alkaline Phosphatase, Tissue Non-Specific mouse mAb (green) and negative control (red).

ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Validation of EV-CATCHER customized antibodies and characterization of placental EVs. (a). Western blots displaying the specificity of the monoclonal placental alkaline phosphatase antibody against the PLAP recombinant protein (Novus, Centennial, CO, USA, #NBP2-52266) used for EV-CATCHER customization versus the alkaline phosphatase, tissue-nonspecific isozyme antibody against the ALPL recombinant protein (Novus, Centennial, CO, USA, #2909-AP-010). (b). EV-CATCHER assay customized with an anti-PLAP antibody conjugated to a uracilated DNA linker that is connected by a biotin to the bottom of a streptavidin coated well. Using uracil DNA glycosylase (UNG), the immobilized EV-anti-PLAP duplex is released by enzymatic digestion of the double-stranded uracilated DNA linker. (c). Representative Transmission electron microscopy (TEM) images of EVs isolated from placenta previa and placenta percreta maternal plasma, scale bar 100 um. (d). Representative ONi super resolution images of EVs isolated from placenta previa and placenta percreta maternal plasma displaying both high PLAP+ expressing EVs and low PLAP+ expressing EVs, scale bar of 20 um for large field-of-view images and 200 nm for individual representative EVs. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38791142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22193-0.025ml

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NBP3-15273

Alkaline Phosphatase/ALPL Antibody (10B1M5)

100 ul

NBP3-15273

Immunohistochemistry: Alkaline Phosphatase/ALPL Antibody (10B1M5) [Alkaline Phosphatase/ALPL] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Alkaline Phosphatase/ALPL Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Western Blot: Alkaline Phosphatase/ALPL Antibody (10B1M5) [NBP3-15273] -Analysis of various lysates using Alkaline Phosphatase (ALPL) Rabbit mAb at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB29091

Alkaline Phosphatase/ALPL Antibody (388828)

(6 Publications)

100 μg

MAB29091

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Alkaline Phosphatase/ALPL was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB29091) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB29091U-100UG
Alexa Fluor 405	FAB29091V-100UG
Alexa Fluor 488	FAB29091G-100UG
Alexa Fluor 532	FAB29091X-100UG
Alexa Fluor 594	FAB29091T-100UG
Alexa Fluor 647	FAB29091R-100UG
Alexa Fluor 700	FAB29091N-100UG
Alexa Fluor 750	FAB29091S-100UG
Alexa Fluor Plus 405	FAB29091AFP405-100UG
Alexa Fluor Plus 488	FAB29091AFP488-100UG
Alexa Fluor Plus 555	FAB29091AFP555-100UG
Alexa Fluor Plus 594	FAB29091AFP594-100UG
Alexa Fluor Plus 647	FAB29091AFP647-100UG
Alexa Fluor Plus 680	FAB29091AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB29091-SP

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2909-AP

Recombinant Human Alkaline Phosphatase/ALPL Protein, CF

(6 Publications)

10 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB2909

Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated]

(5 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2909U-100UG
Alexa Fluor 405	FAB2909V-100UG
Alexa Fluor 488	FAB2909G-100UG
Alexa Fluor 532	FAB2909X-100UG
Alexa Fluor 594	FAB2909T-100UG
Alexa Fluor 647	FAB2909R-100UG
Alexa Fluor 700	FAB2909N-100UG
Alexa Fluor 750	FAB2909S-100UG
Alexa Fluor Plus 405	FAB2909AFP405-100UG
Alexa Fluor Plus 488	FAB2909AFP488-100UG
Alexa Fluor Plus 555	FAB2909AFP555-100UG
Alexa Fluor Plus 594	FAB2909AFP594-100UG
Alexa Fluor Plus 647	FAB2909AFP647-100UG
Alexa Fluor Plus 680	FAB2909AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2909-SP

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NBP1-32948

Alkaline Phosphatase, Tissue Non-Specific Antibody - BSA Free

(1 Publication)

0.1 ml

NBP1-32948

<b>Biological Strategies Validation. </b>Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32948-img0006.jpg

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody (NBP1-32948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Deprivation of NO induces bi-lineage phenotype and expression of stem cell markers in mammary epithelial cells both in vivo and in 3D cultures. (A) CK14 (basal) and CK8/18 (luminal) staining of mouse mammary glands treated with control (PBS), L-arginine or L-NAME for 6 weeks. Co-localization of CK14 and CK8/18 was captured with ImageJ. Scale bars: 100 μm. (See the quantification in Supplementary Fig. 6A). (B) Representative images of drug-treated mammary glands stained for stem cell markers: alkaline phosphatase (AP) (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 μm. (See the quantification in Supplementary Fig. 6B–D). (C) CK14 and CK8/18 staining of non-malignant MCF10A cells cultivated in 3D ECM in the presence of control (PBS), L-arginine or L-NAME for 3 weeks. Scale bars: 50 μm. (See the quantification in Supplementary Fig. 6E). (D) Drug-treated MCF10A cells in 3D cultures stained for AP (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 μm. (See the quantification in Supplementary Fig. 6F–H). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31040372), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Sh, Rt, Fe, RM

Species Glossary

Reactivity: Hu, Mu, Sh, Rt, Fe, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

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Species Glossary

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Recombinant Human Alkaline Phosphatase/ALPL Protein, CF

Alkaline Phosphatase/ALPL Antibody (388816) [Unconjugated]

Alkaline Phosphatase, Tissue Non-Specific Antibody - BSA Free