Products Related to Gene-ID: 22943

3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7 to induce cardiomyocyte differentiation. Cells were then cultured in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond. Cells were fixed and stained for stage-specific markers at select time points during the procedure. The pluripotency marker Oct-4 was detected using a Mouse Anti-Human Oct-4A Monoclonal Antibody (MAB17591). The mesoderm marker, Snail is expressed intermediately during differentiation (Day 1). It was detected using a Goat Anti-Human Snail Polyclonal Antibody (AF3639). The cardiomyocyte markers NKX2.5 and Troponin T are not present in cells during early (Day 0) and intermediate (Day1) differentiation and become more highly expressed during the later stages of differentiation (Day 7, Day 30). NKX2.5 was detected using a Goat Anti-Human NKX2.5 Polyclonal Antibody (AF2444) and Troponin T was detected using a Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (MAB1874). Snail and NKX2.5 primary antibodies were visualized with a NorthernLights™ 557-conjugated Donkey Anti-Goat IgG Secondary Antibody (NL001). Oct-4 and Troponin T were visualized with a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (NL007)." class="big_lightbox"> 3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7 to induce cardiomyocyte differentiation. Cells were then cultured in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond. Cells were fixed and stained for stage-specific markers at select time points during the procedure. The pluripotency marker Oct-4 was detected using a Mouse Anti-Human Oct-4A Monoclonal Antibody (MAB17591). The mesoderm marker, Snail is expressed intermediately during differentiation (Day 1). It was detected using a Goat Anti-Human Snail Polyclonal Antibody (AF3639). The cardiomyocyte markers NKX2.5 and Troponin T are not present in cells during early (Day 0) and intermediate (Day1) differentiation and become more highly expressed during the later stages of differentiation (Day 7, Day 30). NKX2.5 was detected using a Goat Anti-Human NKX2.5 Polyclonal Antibody (AF2444) and Troponin T was detected using a Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (MAB1874). Snail and NKX2.5 primary antibodies were visualized with a NorthernLights™ 557-conjugated Donkey Anti-Goat IgG Secondary Antibody (NL001). Oct-4 and Troponin T were visualized with a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (NL007)." class="big_thumb" />

3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7. Following culture in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond, cells were fixed and stained for the atrial-specific marker, MLC2a, and the ventricle-specific marker, MYH7 using a Rabbit Anti-Human MYH7 Monoclonal Antibody (MAB90961)." class="big_lightbox"> 3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7. Following culture in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond, cells were fixed and stained for the atrial-specific marker, MLC2a, and the ventricle-specific marker, MYH7 using a Rabbit Anti-Human MYH7 Monoclonal Antibody (MAB90961)." class="big_thumb" />

HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse Dkk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for Dkk-1 at approximately 28-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for Dkk-1 at approximately 35 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for Dkk-1 at approximately 35 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1765-DK) was serially diluted and captured by Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1765) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Dkk-1 was detected in immersion fixed paraffin-embedded sections of human colon using Human Dkk-1 Monoclonal Antibody (Catalog # MAB10962) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 5439-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑1 Monoclonal Antibody (Catalog # MAB10962) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Dkk‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1096) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 30-180 ng/mL (orange circles)." class="big_lightbox"> 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 30-180 ng/mL (orange circles)." alt="Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 30-180 ng/mL (orange circles)." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Dkk‑1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c) using Mouse Dkk‑1 Monoclonal Antibody (Catalog # MAB1765) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells in lung. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 50-300 ng/mL (orange circles)." class="big_lightbox"> 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 50-300 ng/mL (orange circles)." alt="Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 50-300 ng/mL (orange circles)." class="big_thumb" />