NKG2D/CD314: Novus Biologicals

MAB139

NKG2D/CD314 Antibody (149810) [Unconjugated]

(1 Review)

100 μg

MAB139

FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # <a class=

FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) gated on CD3- cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox">

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD8 APC-conjugated Monoclonal Antibody (<a class=

FAB1509A) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB139) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG PE-conjugated Monoclonal Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.

CD4+NKG2D+ T cells exert pro-migratory, cytolytic and pro-inflammatory properties.(A) Flow cytometry: The dot plot shows a representative example of NKG2D expression on CD3+CD4+CD8-CD56− T cells derived from the peripheral blood of a healthy donor (HD). The bar graph represents the mean frequency of CD4+NKG2D+ T cells in the peripheral blood of HDs (n = 20), stable RRMS (n = 15) and active RRMS patients (n = 14). (B–E) Mean fluorescence intensity (MFI) of different markers indicative for migratory capacity (B), activation (C), or cytolytic capacity (D, E) of CD4+NKG2D+ and CD4+NKG2D− T cells from the peripheral blood of HDs (n = 6) or active RRMS patients (n = 6). (F) Percentages of naive (CD45RA+CD62L+), T central memory (Tcm, CD45RA-CD62L+), T effector memory (Tem, CD45RA-CD62L−) and T effector memory RA (Tem-RA, CD45RA+CD62L-) cells in the CD4+NKG2D+ and CD4+NKG2D− T cell compartment assessed by flow cytometry (n = 6 HDs). (G) Carboxyfluorescein succinimidyl ester (CFSE) proliferation assays of CD4+NKG2D+ T cells and CD4+NKG2D− T cells under CD3/CD28, CD3/NKG2D, MOG35-55 (10 µg/ml or 100 µg/ml), MBP1-11 or PLP190-209 stimulation (n = 8 HDs). (H) Intracellular cytokine staining for IFN-gamma and IL-17 of CD4+NKG2D+ and CD4+NKG2D− T cells derived from the peripheral blood of HDs (n = 7). The dot plots depict a representative example of IFN-gamma - and IL-17-positive cells upon CD3/CD28 stimulation. The bar graphs show the frequencies of IFN-gamma or IL-17 positive cells of unstimulated, CD3/CD28- or CD3/NKG2D-stimulated cells. (I) Comparison of the proportions of IFN-gamma or IL-17 positive CD3/CD28-stimulated CD4NKG2D+ T cells derived from frozen PBMCs of HDs (n = 6) or active RRMS patients (n = 6). *P < 0.05. ns, not significant; unstim., unstimulated. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24282598), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D and LFA-1 ligation trigger morphologically different responses in NK cells. (H) Quantification of f-actin (phalloidin-AlexaFluor633) across the diameter of cells stimulated on anti-NKG2D (solid line) or anti-LFA-1 (dotted line) (horizontal bars indicate p<0.05 by ANOVA, n = 20). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/19636352), licensed under a CC-BY license. Not internally tested by R&D Systems.

Consequences of MICA-mediated and antibody-mediated NKG2D engagement on cell activation, NKG2D and TIGIT expression. Primary cultured NK cells were incubated with plate-bound recombinant MICA Fc-chimera or anti-NKG2D mAb and goat anti-mouse antibody (GAM). GAM-stimulated NK cells were used as control of NKG2D stimulation. (A) Cells were harvested after 4 hours of stimulation, and degranulation was measured via flow cytometry by assessing CD107a endosomal marker expression. A representative experiment is shown in left panel while means ± SD of three independent experiments are shown in right panel. Paired t test was used for comparisons. *P < 0.05. (B, C) Surface expression of NKG2D and TIGIT receptors was evaluated by FACS analysis upon 18 hours of stimulation. One representative experiment is shown in left panels. Right panels: data pooled from six independent experiments (mean ± SD) are shown (the mean fluorescence intensity, MFI, value of controls NK cells in each experiment is set to 1). Paired t test was used for comparisons. *ρ < 0.05, **ρ < 0.01, ***ρ < 0.001. (D) Relative TIGIT mRNA amount, normalized with GAPDH, was expressed as arbitrary units and referred to the relative controls, considered as calibrator (the value of the calibrator in each run is set to 1). Means ± SD of five independent experiments are shown. Paired t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D stimulation impairs DNAM-1-triggered ERK1/2 phosphorylation. (A, B) goat anti-mouse (GAM) and anti-NKG2D-stimulated NK cells were incubated with anti-DNAM-1 mAb or isotype control Ab followed by goat anti-mouse Ab for different lengths of times. Then, cells were fixed and permeabilized, stained with anti-pAKT (panel A) and anti-pERK1/2 (panel B) and analyzed by FACS. Results from 2 minutes of stimulation with anti-DNAM-1 mAb (filled histograms) or isotype control Ab (empty histograms) from one representative of three independent experiments is shown in the left panels. Quantitative changes in protein phosphorylation at the indicated lengths of time of three independent experiments (mean ± SD) are shown in the right panels. The ratio between the mean fluorescence intensity (MFI) of anti-DNAM-1-stimulated and Ctrl-Ig samples for each time point was used to calculate fold changes. Multiple t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

NKG2D internalization is required for impairment in DNAM-1-mediated cytotoxic granule polarization. (A) Surface expression of NKG2D (upper panels) and TIGIT (lower panels) receptors was evaluated on goat anti-mouse (GAM) or anti-NKG2D-stimulated DAP10 WT and DAP10K84R NKL. One representative of three independent experiments is shown in left panels. Data pooled from three independent experiments (mean ± SD) are shown in right panels (the mean fluorescence intensity, MFI value of GAM-stimulated cells in each experiment is set to 1). Paired t test was used for comparisons. *ρ < 0.05. (B) GAM and anti-NKG2D-stimulated DAP10 WT and DAP10K84R NKL (effector cells, E) were incubated with Ba/F3-PVR (target cells, T) for 30 minutes, fixed, permeabilized and stained with the anti-Perforin mAb followed by AlexaFluor594-conjugated GAM-IgG2b and AlexaFluor488-conjugated Phalloidin to visualize polarized actin. Cells were then counterstained with DAPI to visualize nuclei and acquired with LSM980 confocal microscope with a 63x/1.4 NA UPlanSAPO oil immersion objective (all from Zeiss). Right panel: Percentages of conjugates with polarized granules are shown as mean ± SD calculated on ≥30 conjugates for each NK cell type of three independent experiments. Paired t test was used for comparisons. *ρ < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40421025), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB139U-100UG
Alexa Fluor 405	FAB139V-100UG
Alexa Fluor 488	FAB139G-100UG
Alexa Fluor 532	FAB139X
Alexa Fluor 594	FAB139T-100UG
Alexa Fluor 647	FAB139R-100UG
Alexa Fluor 700	FAB139N-100
Alexa Fluor 700	FAB139N-025
Alexa Fluor 750	FAB139S-100UG
Alexa Fluor Plus 405	FAB139AFP405-100UG
Alexa Fluor Plus 488	FAB139AFP488-100UG
Alexa Fluor Plus 555	FAB139AFP555-100UG
Alexa Fluor Plus 594	FAB139AFP594-100UG
Alexa Fluor Plus 647	FAB139AFP647-100UG
Alexa Fluor Plus 680	FAB139AFP680-100UG
Allophycocyanin	FAB139A-025
Allophycocyanin	FAB139A-100
Allophycocyanin/Cy7	FAB139APCCY7
Biotin	FAB139B
CoraFluor 1	FAB139CL1
DyLight 350	FAB139D
DyLight 405	FAB139E
DyLight 488	FAB139K
DyLight 550	FAB139L
DyLight 594	FAB139M
DyLight 650	FAB139W
DyLight 680	FAB139Y
DyLight 755	FAB139Z
FITC	FAB139F
HRP	FAB139H
Janelia Fluor 525	FAB139JF525
Janelia Fluor 549	FAB139I
Janelia Fluor 646	FAB139J
Janelia Fluor 669	FAB139JF669
PE/Atto594	FAB139PEATT594
PE/Cy5.5	FAB139PECY55
PE/Cy7	FAB139PECY7
Phycoerythrin	FAB139P-100
Phycoerythrin	FAB139P-025
mFluor Violet 450 SE	FAB139MFV450
mFluor Violet 500 SE	FAB139MFV500
mFluor Violet 610 SE	FAB139MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB139G-100UG
Carrier Free	FAB139P-025
Carrier Free	FAB139P-100
Carrier Free	FAB139A-025
Carrier Free	FAB139A-100
Carrier Free	FAB139N-025
Carrier Free	FAB139N-100
Carrier Free	MAB139-SP
Carrier Free	MAB139-500
Carrier Free	FAB139V-100UG
Carrier Free	FAB139U-100UG
Carrier Free	FAB139T-100UG
Carrier Free	FAB139S-100UG
Carrier Free	FAB139R-100UG

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1299-NK

Recombinant Human NKG2D Fc Chimera Protein, CF

(35 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB1547

NKG2D/CD314 Antibody (191004) [Unconjugated]

(21 Publications)

100 μg

MAB1547

NK Require NKG2D to Control Early Virus Dissemination & for Optimal Cytotoxicity but Not Activation(A) B6 mice infected with 3,000 pfu ECTV. On day 5 PI, mice pulsed with BrdU for 3 h & their spleens analyzed by flow cytometry. Plots are gated on CD3ɛ− NK1.1+ . Data correspond to pools of three mice from three individual experiments.(B) Increased viral titers of infected mice with NKG2D blockade in vivo. Intact B6 mice, B6 mice with NKG2D blockade, B6 mice depleted of NK , B6 mice depleted of T (treated with anti-CD4 + anti-CD8 mAbs), or depleted of T & with NKG2D blockade infected with 3,000 pfu ECTV & the viral titers in spleen determined on day 3 PI. Data are the average ± SD of three individual mice & are representative of two similar experiments.(C) The indicated mice infected with 3,000 pfu ECTV, & the NK responses in the D-LN determined at 2 d PI. Upper panel: Dot plot showing the proportion of NK (DX5+, CD3e−) in the D-LN of infected & control uninfected mice. Lower panel: GzB & IFN-gamma production by NK . correspond to the DX5+, CD3ɛ− gate of the upper panels. Data correspond to pools of three mice & are representative of three similar experiments.(D) NK purified from spleens (5 d PI) of ECTV-infected intact or NKG2D-blocked mice & stained as indicated. Filled histogram: isotype-matched control Ig; gray line: NKG2D-blocked mice; black line: intact mice.(E) NK purified from spleens of intact or NKG2D-blocked mice as indicated & used as effectors in a 4-h 51Cr release assay against the indicated targets. Data correspond to pools of three mice & are representative of three experiments.(F) As in (D), but the NK purified from untreated mice & a neutralizing anti-NKG2D mAb was added to the in vitro cytotoxicity assays, as indicated. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/18266471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1547U-100UG
Alexa Fluor 405	FAB1547V-100UG
Alexa Fluor 488	FAB1547G-100UG
Alexa Fluor 532	FAB1547X
Alexa Fluor 594	FAB1547T-100UG
Alexa Fluor 647	FAB1547R-100UG
Alexa Fluor 700	FAB1547N-100UG
Alexa Fluor 750	FAB1547S-100UG
Alexa Fluor Plus 405	FAB1547AFP405-100UG
Alexa Fluor Plus 488	FAB1547AFP488-100UG
Alexa Fluor Plus 555	FAB1547AFP555-100UG
Alexa Fluor Plus 594	FAB1547AFP594-100UG
Alexa Fluor Plus 647	FAB1547AFP647-100UG
Alexa Fluor Plus 680	FAB1547AFP680-100UG
Allophycocyanin/Cy7	FAB1547APCCY7
Biotin	BAM1547
CoraFluor 1	FAB1547CL1
DyLight 350	FAB1547D
DyLight 405	FAB1547E
DyLight 488	FAB1547K
DyLight 550	FAB1547L
DyLight 594	FAB1547M
DyLight 650	FAB1547W
DyLight 680	FAB1547Y
DyLight 755	FAB1547Z
HRP	FAB1547H
Janelia Fluor 525	FAB1547JF525
Janelia Fluor 549	FAB1547I
Janelia Fluor 646	FAB1547J
Janelia Fluor 669	FAB1547JF669
PE/Atto594	FAB1547PEATT594
PE/Cy5.5	FAB1547PECY55
PE/Cy7	FAB1547PECY7
PerCP	FAB1547C
Phycoerythrin	FAB1547P-100UG
mFluor Violet 450 SE	FAB1547MFV450
mFluor Violet 500 SE	FAB1547MFV500
mFluor Violet 610 SE	FAB1547MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1547-SP
Carrier Free	MAB1547-500
Carrier Free	FAB1547V-100UG
Carrier Free	FAB1547U-100UG
Carrier Free	FAB1547T-100UG
Carrier Free	FAB1547S-100UG
Carrier Free	FAB1547R-100UG
Carrier Free	FAB1547N-100UG
Carrier Free	FAB1547G-100UG
Carrier Free	FAB1547P-100UG

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139-NK

Recombinant Mouse NKG2D Fc Chimera Protein, CF

(20 Publications)

50 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NB100-65956

NKG2D/CD314 Antibody (1D11) - Azide and BSA Free

(1 Review)

(3 Publications)

0.25 mg

NB100-65956

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody,

Immunohistochemistry-Frozen: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody.

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD314:Low Endotoxin.

Flow Cytometry: NKG2D/CD314 Antibody (1D11) [NB100-65956] - Human peripheral blood lymphocytes.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-65956AF350
Alexa Fluor 405	NB100-65956AF405
Alexa Fluor 488	NB100-65956AF488
Alexa Fluor 532	NB100-65956AF532
Alexa Fluor 594	NB100-65956AF594
Alexa Fluor 647	NB100-65956AF647
Alexa Fluor 700	NB100-65956AF700
Alexa Fluor 750	NB100-65956AF750
Allophycocyanin	NB100-65956APC
Biotin	NB100-65956B
CoraFluor 1	NB100-65956CL1
DyLight 350	NB100-65956UV
DyLight 405	NB100-65956V
DyLight 488	NB100-65956G
DyLight 550	NB100-65956R
DyLight 594	NB100-65956DL594
DyLight 650	NB100-65956C
DyLight 680	NB100-65956FR
DyLight 755	NB100-65956IR
FITC	NB100-65956F
HRP	NB100-65956H
Janelia Fluor 525	NB100-65956JF525
Janelia Fluor 549	NB100-65956JF549
Janelia Fluor 646	NB100-65956JF646
Janelia Fluor 669	NB100-65956JF669
PE	NB100-65956PE
PerCP	NB100-65956PCP
mFluor Violet 450 SE	NB100-65956MFV450
mFluor Violet 500 SE	NB100-65956MFV500
mFluor Violet 610 SE	NB100-65956MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB100-65956-0.05mg

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NBP2-43645

NKG2D/CD314 Antibody - BSA Free

0.1 ml

NBP2-43645

Immunocytochemistry/ Immunofluorescence: NKG2D/CD314 Antibody [NBP2-43645] - NKG2D/CD314 antibody [N3C2], Internal detects NKG2D/CD314 protein at cell membrane by immunofluorescent analysis.</br>Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: NKG2D/CD314 stained by NKG2D/CD314 antibody [N3C2], Internal (NBP2-43645) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .</br>Scale bar= 10um.

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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10960-NK

Recombinant Cynomolgus/Rhesus NKG2D Fc Chimera Protein, CF

50 μg

10960-NK

AVI1300) binds with an ED₅₀ of 25.0-200 ng/mL." class="big_lightbox">

AVI1300) binds with an ED₅₀ of 25.0-200 ng/mL." alt="When Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human MICA Fc Chimera Avi-tag (AVI1300) binds with an ED₅₀ of 25.0-200 ng/mL." class="big_thumb" />

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque NKG2D/CD314 Fc Chimera Protein (Catalog # 10960-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

Reactivity:

Pm-Cm, RM

Species Glossary

Reactivity: Pm-Cm, RM

Applications:

Applications:

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MAB1391

NKG2D/CD314 Antibody (2855A) [Unconjugated]

(1 Publication)

100 μg

MAB1391

202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10ng/ml Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with either (A) Rabbit Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1391) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

<b>Biological Strategies Validation. </b>Day 10 activated and expanded NK cells with CD56 costain treated with 27 ng/ml Recombinant Human IL-2 Protein (<a class=

202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10ng/ml Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with either (A) Rabbit Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1391) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins. " alt="Day 10 activated and expanded NK cells with CD56 costain treated with 27 ng/ml Recombinant Human IL-2 Protein (202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10ng/ml Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with either (A) Rabbit Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1391) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1391U-100UG
Alexa Fluor 405	FAB1391V-100UG
Alexa Fluor 488	FAB1391G-100UG
Alexa Fluor 532	FAB1391X
Alexa Fluor 594	FAB1391T-100UG
Alexa Fluor 647	FAB1391R-100UG
Alexa Fluor 700	FAB1391N-100
Alexa Fluor 750	FAB1391S-100UG
Alexa Fluor Plus 405	FAB1391AFP405-100UG
Alexa Fluor Plus 488	FAB1391AFP488-100UG
Alexa Fluor Plus 555	FAB1391AFP555-100UG
Alexa Fluor Plus 594	FAB1391AFP594-100UG
Alexa Fluor Plus 647	FAB1391AFP647-100UG
Alexa Fluor Plus 680	FAB1391AFP680-100UG
Allophycocyanin	FAB1391A
Allophycocyanin/Cy7	FAB1391APCCY7
Biotin	FAB1391B
CoraFluor 1	FAB1391CL1
DyLight 350	FAB1391D
DyLight 405	FAB1391E
DyLight 488	FAB1391K
DyLight 550	FAB1391L
DyLight 594	FAB1391M
DyLight 650	FAB1391W
DyLight 680	FAB1391Y
DyLight 755	FAB1391Z
FITC	FAB1391F
HRP	FAB1391H
Janelia Fluor 525	FAB1391JF525
Janelia Fluor 549	FAB1391I
Janelia Fluor 646	FAB1391J
Janelia Fluor 669	FAB1391JF669
PE	FAB1391P
PE/Atto594	FAB1391PEATT594
PE/Cy5.5	FAB1391PECY55
PE/Cy7	FAB1391PECY7
PerCP	FAB1391C
mFluor Violet 450 SE	FAB1391MFV450
mFluor Violet 500 SE	FAB1391MFV500
mFluor Violet 610 SE	FAB1391MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1391R-100UG
Carrier Free	MAB1391-SP
Carrier Free	FAB1391V-100UG
Carrier Free	FAB1391T-100UG
Carrier Free	FAB1391U-100UG
Carrier Free	FAB1391S-100UG
Carrier Free	FAB1391G-100UG
Carrier Free	FAB1391N-100

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MAB1392

NKG2D/CD314 Antibody (1046002) [Unconjugated]

100 μg

MAB1392

202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10 ng/ml Recombinant Human IL-21 Protein (8879-IL) for 15 days) were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Rabbit IgG Alexa Fluor® 700-conjugated Antibody (Catalog # IC1051N). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

NK cells (PBMC treated with 27 ng/ml Recombinant Human IL-2 Protein (<a class=

202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10 ng/ml Recombinant Human IL-21 Protein (8879-IL) for 15 days) were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Rabbit IgG Alexa Fluor® 700-conjugated Antibody (Catalog # IC1051N). View our protocol for Staining Membrane-associated Proteins. " alt="NK cells (PBMC treated with 27 ng/ml Recombinant Human IL-2 Protein (202-IL), 10 ng/ml Recombinant Human IL-12 Protein (219-IL), 10 ng/ml Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and 10 ng/ml Recombinant Human IL-21 Protein (8879-IL) for 15 days) were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Rabbit IgG Alexa Fluor® 700-conjugated Antibody (Catalog # IC1051N). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

CD3- CD56+ naïve PBMC were stained with Rabbit Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # <a class=

FAB24086P) and either (A) Mouse Anti-Human NKG2D/CD314 Monoclonal Antibody (Catalog # MAB1392) or (B) Mouse IgG_2B Isotype Control (Catalog # MAB004) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1392U-100UG
Alexa Fluor 405	FAB1392V-100UG
Alexa Fluor 488	FAB1392G-100UG
Alexa Fluor 532	FAB1392X
Alexa Fluor 594	FAB1392T-100UG
Alexa Fluor 647	FAB1392R-100UG
Alexa Fluor 700	FAB1392N-100UG
Alexa Fluor 750	FAB1392S-100UG
Alexa Fluor Plus 405	FAB1392AFP405-100UG
Alexa Fluor Plus 488	FAB1392AFP488-100UG
Alexa Fluor Plus 555	FAB1392AFP555-100UG
Alexa Fluor Plus 594	FAB1392AFP594-100UG
Alexa Fluor Plus 647	FAB1392AFP647-100UG
Alexa Fluor Plus 680	FAB1392AFP680-100UG
Allophycocyanin	FAB1392A-100
Allophycocyanin/Cy7	FAB1392APCCY7
Biotin	FAB1392B
CoraFluor 1	FAB1392CL1
DyLight 350	FAB1392D
DyLight 405	FAB1392E
DyLight 488	FAB1392K
DyLight 550	FAB1392L
DyLight 594	FAB1392M
DyLight 650	FAB1392W
DyLight 680	FAB1392Y
DyLight 755	FAB1392Z
FITC	FAB1392F
HRP	FAB1392H
Janelia Fluor 525	FAB1392JF525
Janelia Fluor 549	FAB1392I
Janelia Fluor 646	FAB1392J
Janelia Fluor 669	FAB1392JF669
PE	FAB1392P
PE/Atto594	FAB1392PEATT594
PE/Cy5.5	FAB1392PECY55
PE/Cy7	FAB1392PECY7
PerCP	FAB1392C
mFluor Violet 450 SE	FAB1392MFV450
mFluor Violet 500 SE	FAB1392MFV500
mFluor Violet 610 SE	FAB1392MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1392V-100UG
Carrier Free	FAB1392S-100UG
Carrier Free	FAB1392G-100UG
Carrier Free	MAB1392-SP
Carrier Free	FAB1392U-100UG
Carrier Free	FAB1392R-100UG
Carrier Free	FAB1392T-100UG
Carrier Free	FAB1392N-100UG
Carrier Free	FAB1392A-100

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NB100-1891

NKG2D/CD314 Antibody

(2 Publications)

0.1 mg

NB100-1891

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP3-20156

NKG2D/CD314 Antibody (6E5A7) - Chimeric - Azide and BSA Free

0.2 mg

NBP3-20156

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-20156AF350
Alexa Fluor 405	NBP3-20156AF405
Alexa Fluor 488	NBP3-20156AF488
Alexa Fluor 532	NBP3-20156AF532
Alexa Fluor 594	NBP3-20156AF594
Alexa Fluor 647	NBP3-20156AF647
Alexa Fluor 700	NBP3-20156AF700
Alexa Fluor 750	NBP3-20156AF750
Alexa Fluor Plus 405	NBP3-20156AFP405
Alexa Fluor Plus 488	NBP3-20156AFP488
Alexa Fluor Plus 555	NBP3-20156AFP555
Alexa Fluor Plus 594	NBP3-20156AFP594
Alexa Fluor Plus 647	NBP3-20156AFP647
Alexa Fluor Plus 680	NBP3-20156AFP680
Allophycocyanin	NBP3-20156APC
Biotin	NBP3-20156B
CoraFluor 1	NBP3-20156CL1
DyLight 350	NBP3-20156UV
DyLight 405	NBP3-20156V
DyLight 488	NBP3-20156G
DyLight 550	NBP3-20156R
DyLight 594	NBP3-20156DL594
DyLight 650	NBP3-20156C
DyLight 680	NBP3-20156FR
DyLight 755	NBP3-20156IR
FITC	NBP3-20156F
HRP	NBP3-20156H
Janelia Fluor 525	NBP3-20156JF525
Janelia Fluor 549	NBP3-20156JF549
Janelia Fluor 646	NBP3-20156JF646
Janelia Fluor 669	NBP3-20156JF669
PE	NBP3-20156PE
PerCP	NBP3-20156PCP
mFluor Violet 450 SE	NBP3-20156MFV450
mFluor Violet 500 SE	NBP3-20156MFV500
mFluor Violet 610 SE	NBP3-20156MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-20156-0.025mg

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NBP3-41507

NKG2D/CD314 Antibody (C1) - BSA Free

100 ul

NBP3-41507

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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