CNTFR alpha: Novus Biologicals

AF-303-NA

CNTFR alpha Antibody [Unconjugated]

(1 Review)

(8 Publications)

100 μg

AF-303-NA

Recombinant Human CNTF R alpha (Catalog # 303‑CR) enhances Recombinant Human CNTF (Catalog # 257-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recombinant Human CNTF R alpha (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑303‑NA). The ND50 is typically 10‑20 µg/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF303

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-303-SP

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AF-559-NA

CNTFR alpha Antibody [Unconjugated]

(3 Publications)

100 μg

AF-559-NA

Recombinant Rat CNTF R alpha (Catalog # 558-CR) enhances Recombinant Rat CNTF (Catalog # 557-NT) induced proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Rat CNTF (10 ng/mL) activity elicited by Recombinant Rat CNTF R alpha (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑559‑NA). The ND50 is typically 2‑6 µg/mL.

CNTF activates tyrosine hydroxylase by phosphorylation in mouse and human locus coeruleusCntfr mRNA expression in mouse locus coeruleus (LC, white arrows). Increasing mRNA content is indicated by a color gradient ranging from dark blue (low) to red (high). The ependymal surface as contact point with the 4th ventricle is shown in blue. Scale bar = 100 μm.Hypothetical orientation of NE neurons with their dendrites emanating toward the 4th ventricle. (B1) CNTF receptor (CNTFR) localization in tyrosine hydroxylase (TH)+ processes extending toward the ventricular wall (arrow marks ventricular surface). Scale bar = 10 μm.c‐Fos activation in LC 2 h after formalin stress. Left: Increased density of c‐Fos+ cells in the LC. Scale bar = 150 μm. Right: Quantitative data. *P 4 mice/genotype.Data information: Data are expressed as means ± s.e.m. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30209240), licensed under a CC-BY license. Not internally tested by R&D Systems.

$Secretagogin expression in rodent brain stem (related to Fig 4)A, A1Labeling specificity of our anti‐secretagogin antibody was verified in Scgn−/− mice (A1). These experiments also showed that tyrosine hydroxylase (TH)+ LC neurons (*) co‐express secretagogin in wild‐type (solid arrowheads) but not null mice (open arrowheads). Scale bars = 200 μm (low‐power micrographs, left) and 10 μm (high‐resolution insets, right).BSecretagogin+ neurons populate the locus coeruleus (LC) in the rat. Solid red circles pinpoint the general location of perikarya, while fine lines correspond to axons coursing toward the surface (4th ventricle).C, C1Secretagogin was expressed in both the somata (arrowheads; C) and dendrites (arrowheads; C1) of most TH+ neurons in the rat, too. Scale bars = 30 μm.DSecretagogin is localized to axons (arrowheads) in the rat. (D) Dorsal nucleus of vagus (nX), “2” shows the nucleus at high resolution. Scale bars = 200 μm (D), 30 μm (2).ESecretagogin (SCGN) is enriched in the synaptosomal fraction prepared from the mouse prefrontal cortex, lending additional support for its axonal localization. Synaptophysin (SYP) was used to control the enrichment of presynaptic proteins.FSecretagogin+ neurons can co‐express neuropeptide Y (NPY) in the LC of the rat. Open and solid arrowheads indicate the lack and existence of NPY co‐localization in adjacent neurons, respectively. Scale bars = 10 μm.GRepresentative Western blots from Cntf−/−, Scgn−/−, and wild‐type mice with or without intracerebroventricular CNTF treatment.H, H1INS‐1E cells were used as a cellular model exclusively because of their native co‐expression of CNTF receptor‐ alpha (CNTFR alpha ), tyrosine hydroxylase (TH), secretagogin (SCGN), and extracellular signal‐regulated kinase 1/2 (Erk1/2). Red arrows label target proteins at their predicted sizes. Multiple bands likely represent splice variants, posttranslational modifications, or multimers. Antibodies were also validated histochemically often using knock‐out tissues.ISecretagogin, phospho‐Ser31‐TH, phospho‐Erk1, and total Erk1 expression in INS‐1E cells stimulated with CNTF with/without concomitant silencing of secretagogin (Scgn) gene expression (for 48 h). Note the lack of Erk1 phosphorylation upon genetic inactivation of secretagogin.JSignificant reduction of secretagogin (SCGN) protein levels upon acute RNAi‐mediated gene silencing in vitro (for 48 h). CNTF exposure did not affect the efficacy of gene silencing. Experiments were performed in triplicate; *P 4 mice/genotype.Data information: Data are expressed as means ± s.e.m. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30209240), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF559

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-559-SP

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MAB303

CNTFR alpha Antibody (121723) [Unconjugated]

(1 Publication)

500 μg

MAB303

CNTFR alpha was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human CNTFR alpha Monoclonal Antibody (Catalog # MAB303) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma and sarcoplasm of skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB303-SP

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558-CR

Recombinant Rat CNTFR alpha Protein

(2 Publications)

50 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	558-CR-050/CF

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303-CR

Recombinant Human CNTFR alpha Protein

(1 Publication)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	303-CR-050/CF

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2415-CR/CF

Recombinant Human CLC/CNTFR alpha Chimera Protein, CF

25 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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2415-CR

Recombinant Human CLC/CNTFR alpha Chimera Protein

25 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	2415-CR-025/CF

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NB100-55418

CNTFR Antibody

(1 Publication)

0.1 mg

NB100-55418

Reactivity:

Mu, Hu, Rt, Ca

Species Glossary

Reactivity: Mu, Hu, Rt, Ca

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP1-68943

CNTFR Antibody - BSA Free

100 ul

NBP1-68943

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-12196

CNTFR Antibody (MM0190-10L29) - Azide and BSA Free

0.1 mg

NBP2-12196

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-12196AF350
Alexa Fluor 405	NBP2-12196AF405
Alexa Fluor 488	NBP2-12196AF488
Alexa Fluor 532	NBP2-12196AF532
Alexa Fluor 594	NBP2-12196AF594
Alexa Fluor 647	NBP2-12196AF647
Alexa Fluor 700	NBP2-12196AF700
Alexa Fluor 750	NBP2-12196AF750
Allophycocyanin	NBP2-12196APC
Biotin	NBP2-12196B
CoraFluor 1	NBP2-12196CL1
DyLight 350	NBP2-12196UV
DyLight 405	NBP2-12196V
DyLight 488	NBP2-12196G
DyLight 550	NBP2-12196R
DyLight 594	NBP2-12196DL594
DyLight 650	NBP2-12196C
DyLight 680	NBP2-12196FR
DyLight 755	NBP2-12196IR
FITC	NBP2-12196F
HRP	NBP2-12196H
Janelia Fluor 525	NBP2-12196JF525
Janelia Fluor 549	NBP2-12196JF549
Janelia Fluor 646	NBP2-12196JF646
Janelia Fluor 669	NBP2-12196JF669
PE	NBP2-12196PE
PerCP	NBP2-12196PCP
mFluor Violet 450 SE	NBP2-12196MFV450
mFluor Violet 500 SE	NBP2-12196MFV500
mFluor Violet 610 SE	NBP2-12196MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-12196-0.025mg

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NBP2-92414

CNTFR Antibody - BSA Free

0.1 ml

NBP2-92414

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-92414-0.02ml

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NBP3-47566

CNTFR Antibody - BSA Free

100 μg

NBP3-47566

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

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Modification

Clonality

Clone

Gene Symbol

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Has Sample Size

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Species Glossary

Products Related to Gene-ID: 1271

CNTFR alpha Antibody [Unconjugated]

CNTFR alpha Antibody [Unconjugated]

CNTFR alpha Antibody (121723) [Unconjugated]

Recombinant Rat CNTFR alpha Protein

Recombinant Human CNTFR alpha Protein

Recombinant Human CLC/CNTFR alpha Chimera Protein, CF

Recombinant Human CLC/CNTFR alpha Chimera Protein

CNTFR Antibody

CNTFR Antibody - BSA Free

CNTFR Antibody (MM0190-10L29) - Azide and BSA Free

CNTFR Antibody - BSA Free

CNTFR Antibody - BSA Free