CNTFR alpha: Novus Biologicals

AF-303-NA

CNTFR alpha Antibody [Unconjugated]

(1 Review)

100 μg

AF-303-NA

303-CR) enhances Recom-binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom-binant Human CNTF Ra (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Ra Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-303-NA). The ND₅₀ is typically 10-20 µg/mL." class="big_lightbox">

Recombinant Human CNTF Ra (Catalog # <a class=

303-CR) enhances Recom-binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom-binant Human CNTF Ra (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Ra Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-303-NA). The ND₅₀ is typically 10-20 µg/mL." alt="Recombinant Human CNTF Ra (Catalog # 303-CR) enhances Recom-binant Human CNTF (Catalog # 257-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human CNTF (20 ng/mL) activity elicited by Recom-binant Human CNTF Ra (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Ra Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF-303-NA). The ND₅₀ is typically 10-20 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-303-NAU-100UG
Alexa Fluor 405	AF-303-NAV-100UG
Alexa Fluor 488	AF-303-NAG-100UG
Alexa Fluor 532	AF-303-NAX-100UG
Alexa Fluor 594	AF-303-NAT-100UG
Alexa Fluor 647	AF-303-NAR-100UG
Alexa Fluor 700	AF-303-NAN-100UG
Alexa Fluor 750	AF-303-NAS-100UG
Alexa Fluor Plus 405	AF-303-NAAFP405
Alexa Fluor Plus 488	AF-303-NAAFP488
Alexa Fluor Plus 555	AF-303-NAAFP555
Alexa Fluor Plus 594	AF-303-NAAFP594
Alexa Fluor Plus 647	AF-303-NAAFP647
Alexa Fluor Plus 680	AF-303-NAAFP680
Allophycocyanin	AF-303-NAA
Biotin	BAF303
CoraFluor 1	AF-303-NACL1
DyLight 350	AF-303-NAD
DyLight 405	AF-303-NAE
DyLight 488	AF-303-NAK
DyLight 550	AF-303-NAL
DyLight 594	AF-303-NAM
DyLight 650	AF-303-NAW
DyLight 680	AF-303-NAY
DyLight 755	AF-303-NAZ
FITC	AF-303-NAF
HRP	AF-303-NAH
Janelia Fluor 525	AF-303-NAJF525
Janelia Fluor 549	AF-303-NAI
Janelia Fluor 646	AF-303-NAJ
Janelia Fluor 669	AF-303-NAJF669
PE	AF-303-NAP
PerCP	AF-303-NAC
mFluor Violet 450 SE	AF-303-NAMFV450
mFluor Violet 500 SE	AF-303-NAMFV500
mFluor Violet 610 SE	AF-303-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-303-SP

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AF-559-NA

CNTFR alpha Antibody [Unconjugated]

(3 Publications)

100 μg

AF-559-NA

558-CR) enhances Recombinant Rat CNTF (Catalog # 557-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Rat CNTF (10 ng/mL) activity elicited by Recombinant Rat CNTF Ra (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-559-NA). The ND₅₀ is typically 2-6 µg/mL." class="big_lightbox">

Recombinant Rat CNTF Ra (Catalog # <a class=

558-CR) enhances Recombinant Rat CNTF (Catalog # 557-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Rat CNTF (10 ng/mL) activity elicited by Recombinant Rat CNTF Ra (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-559-NA). The ND₅₀ is typically 2-6 µg/mL." alt="Recombinant Rat CNTF Ra (Catalog # 558-CR) enhances Recombinant Rat CNTF (Catalog # 557-NT) induced proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Rat CNTF (10 ng/mL) activity elicited by Recombinant Rat CNTF Ra (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-559-NA). The ND₅₀ is typically 2-6 µg/mL." class="big_thumb" />

CNTF activates tyrosine hydroxylase by phosphorylation in mouse and human locus coeruleusCntfr mRNA expression in mouse locus coeruleus (LC, white arrows). Increasing mRNA content is indicated by a color gradient ranging from dark blue (low) to red (high). The ependymal surface as contact point with the 4th ventricle is shown in blue. Scale bar = 100 μm.Hypothetical orientation of NE neurons with their dendrites emanating toward the 4th ventricle. (B1) CNTF receptor (CNTFR) localization in tyrosine hydroxylase (TH)+ processes extending toward the ventricular wall (arrow marks ventricular surface). Scale bar = 10 μm.c‐Fos activation in LC 2 h after formalin stress. Left: Increased density of c‐Fos+ cells in the LC. Scale bar = 150 μm. Right: Quantitative data. *P < 0.05, n = 4/group.Representative Western blots of ex vivo LC explants acutely exposed to recombinant CNTF (for 20 min) show increased TH (D1) and Erk1 (D2) phosphorylation (n ≥ 3 samples/group). *P < 0.05.Intracerebroventricular infusion of Cntf siRNA blunts stress‐induced TH phosphorylation in the LC. (E1) Acute stress was triggered by injecting 4% PFA into a left hind paw unilaterally. Sampling followed 20 min later. *P < 0.05, n = 8/group.Schematic diagram of the BDA‐based retrograde labeling of LC neurons projecting to the mPFC. (F1) Multiple‐labeling immunofluorescence detection of CNTFRs in a BDA+/TH+ neuron. Solid arrowheads point to perisomatic CNTFRs, and open arrowheads label BDA accumulation, while asterisks identify BDA−/TH+ neurons. Scale bar = 7 μm. (F2) Quantitative analysis of CNTFR density on the plasmalemmal surface of BDA− vs. BDA+ NE neurons in n = 4 rats. *P < 0.05.Graphical rendering of the experimental outline to test CNTF effects on NE neurons projecting to the mPFC. GCaMP6f was microinjected into the mPFC in vivo with exogenous CNTF (2 nM) applied to brain slices containing the LC 14 days later. (G1) Representative traces of GCaMP6f fluorescence changes in two NE neurons in respons

$<b>Biological Strategies Validation. </b>Secretagogin expression in rodent brain stem (related to Fig 4)A, A1Labeling specificity of our anti‐secretagogin antibody was verified in Scgn−/− mice (A1). These experiments also showed that tyrosine hydroxylase (TH)+ LC neurons (*) co‐express secretagogin in wild‐type (solid arrowheads) but not null mice (open arrowheads). Scale bars = 200 μm (low‐power micrographs, left) and 10 μm (high‐resolution insets, right).BSecretagogin+ neurons populate the locus coeruleus (LC) in the rat. Solid red circles pinpoint the general location of perikarya, while fine lines correspond to axons coursing toward the surface (4th ventricle).C, C1Secretagogin was expressed in both the somata (arrowheads; C) and dendrites (arrowheads; C1) of most TH+ neurons in the rat, too. Scale bars = 30 μm.DSecretagogin is localized to axons (arrowheads) in the rat. (D) Dorsal nucleus of vagus (nX), “2” shows the nucleus at high resolution. Scale bars = 200 μm (D), 30 μm (2).ESecretagogin (SCGN) is enriched in the synaptosomal fraction prepared from the mouse prefrontal cortex, lending additional support for its axonal localization. Synaptophysin (SYP) was used to control the enrichment of presynaptic proteins.FSecretagogin+ neurons can co‐express neuropeptide Y (NPY) in the LC of the rat. Open and solid arrowheads indicate the lack and existence of NPY co‐localization in adjacent neurons, respectively. Scale bars = 10 μm.GRepresentative Western blots from Cntf−/−, Scgn−/−, and wild‐type mice with or without intracerebroventricular CNTF treatment.H, H1INS‐1E cells were used as a cellular model exclusively because of their native co‐expression of CNTF receptor‐ alpha (CNTFR alpha ), tyrosine hydroxylase (TH), secretagogin (SCGN), and extracellular signal‐regulated kinase 1/2 (Erk1/2). Red arrows label target proteins at their predicted sizes. Multiple bands likely represent splice variants, posttranslational modifications, or multimers. Antibodies were also validated histochemically often using knock‐out tiss$

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-559-NAU-100UG
Alexa Fluor 405	AF-559-NAV-100UG
Alexa Fluor 488	AF-559-NAG-100UG
Alexa Fluor 532	AF-559-NAX-100UG
Alexa Fluor 594	AF-559-NAT-100UG
Alexa Fluor 647	AF-559-NAR-100UG
Alexa Fluor 700	AF-559-NAN-100UG
Alexa Fluor 750	AF-559-NAS-100UG
Alexa Fluor Plus 405	AF-559-NAAFP405
Alexa Fluor Plus 488	AF-559-NAAFP488
Alexa Fluor Plus 555	AF-559-NAAFP555
Alexa Fluor Plus 594	AF-559-NAAFP594
Alexa Fluor Plus 647	AF-559-NAAFP647
Alexa Fluor Plus 680	AF-559-NAAFP680
Allophycocyanin	AF-559-NAA
Biotin	BAF559
CoraFluor 1	AF-559-NACL1
DyLight 350	AF-559-NAD
DyLight 405	AF-559-NAE
DyLight 488	AF-559-NAK
DyLight 550	AF-559-NAL
DyLight 594	AF-559-NAM
DyLight 650	AF-559-NAW
DyLight 680	AF-559-NAY
DyLight 755	AF-559-NAZ
FITC	AF-559-NAF
HRP	AF-559-NAH
Janelia Fluor 525	AF-559-NAJF525
Janelia Fluor 549	AF-559-NAI
Janelia Fluor 646	AF-559-NAJ
Janelia Fluor 669	AF-559-NAJF669
PE	AF-559-NAP
PerCP	AF-559-NAC
mFluor Violet 450 SE	AF-559-NAMFV450
mFluor Violet 500 SE	AF-559-NAMFV500
mFluor Violet 610 SE	AF-559-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-559-SP

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MAB303

CNTFR alpha Antibody (121723) [Unconjugated]

(1 Publication)

500 μg

MAB303

CNTFR alpha was detected in immersion fixed paraffin-embedded sections of Human Skeletal Muscle using Mouse Anti-Human CNTFR alpha Monoclonal Antibody (Catalog # MAB303) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sarcolemma and sarcoplasm of skeletal muscle cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB303U-100UG
Alexa Fluor 405	FAB303V-100UG
Alexa Fluor 488	FAB303G-100UG
Alexa Fluor 532	FAB303X-100UG
Alexa Fluor 594	FAB303T-100UG
Alexa Fluor 647	FAB303R-100UG
Alexa Fluor 700	FAB303N-100UG
Alexa Fluor 750	FAB303S-100UG
Alexa Fluor Plus 405	FAB303AFP405-100UG
Alexa Fluor Plus 488	FAB303AFP488-100UG
Alexa Fluor Plus 555	FAB303AFP555-100UG
Alexa Fluor Plus 594	FAB303AFP594-100UG
Alexa Fluor Plus 647	FAB303AFP647-100UG
Alexa Fluor Plus 680	FAB303AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB303-SP

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558-CR

Recombinant Rat CNTFR alpha Protein

(2 Publications)

50 μg

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	558-CR-050/CF

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303-CR

Recombinant Human CNTFR alpha Protein

(1 Publication)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	303-CR-050/CF

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2415-CR

Recombinant Human CLC/CNTFR alpha Chimera Protein

25 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	2415-CR-025/CF

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NB100-55418

CNTFR Antibody

(1 Publication)

0.1 mg

NB100-55418

Reactivity:

Mu, Hu, Rt, Ca

Species Glossary

Reactivity: Mu, Hu, Rt, Ca

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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NBP1-68943

CNTFR Antibody - BSA Free

100 ul

NBP1-68943

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-92414

CNTFR Antibody - BSA Free

0.1 ml

NBP2-92414

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-92414-0.02ml

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NBP2-12196

CNTFR Antibody (MM0190-10L29) - Azide and BSA Free

0.1 mg

NBP2-12196

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-12196AF350
Alexa Fluor 405	NBP2-12196AF405
Alexa Fluor 488	NBP2-12196AF488
Alexa Fluor 532	NBP2-12196AF532
Alexa Fluor 594	NBP2-12196AF594
Alexa Fluor 647	NBP2-12196AF647
Alexa Fluor 700	NBP2-12196AF700
Alexa Fluor 750	NBP2-12196AF750
Alexa Fluor Plus 405	NBP2-12196AFP405
Alexa Fluor Plus 488	NBP2-12196AFP488
Alexa Fluor Plus 555	NBP2-12196AFP555
Alexa Fluor Plus 594	NBP2-12196AFP594
Alexa Fluor Plus 647	NBP2-12196AFP647
Alexa Fluor Plus 680	NBP2-12196AFP680
Allophycocyanin	NBP2-12196APC
Biotin	NBP2-12196B
CoraFluor 1	NBP2-12196CL1
DyLight 350	NBP2-12196UV
DyLight 405	NBP2-12196V
DyLight 488	NBP2-12196G
DyLight 550	NBP2-12196R
DyLight 594	NBP2-12196DL594
DyLight 650	NBP2-12196C
DyLight 680	NBP2-12196FR
DyLight 755	NBP2-12196IR
FITC	NBP2-12196F
HRP	NBP2-12196H
Janelia Fluor 525	NBP2-12196JF525
Janelia Fluor 549	NBP2-12196JF549
Janelia Fluor 646	NBP2-12196JF646
Janelia Fluor 669	NBP2-12196JF669
PE	NBP2-12196PE
PerCP	NBP2-12196PCP
mFluor Violet 450 SE	NBP2-12196MFV450
mFluor Violet 500 SE	NBP2-12196MFV500
mFluor Violet 610 SE	NBP2-12196MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-12196-0.025mg

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NBP3-47566

CNTFR Antibody - BSA Free

100 μg

NBP3-47566

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-92702

CNTFR Antibody - BSA Free

0.1 ml

NBP2-92702

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-92702-0.02ml

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 1271

CNTFR alpha Antibody [Unconjugated]

CNTFR alpha Antibody [Unconjugated]

CNTFR alpha Antibody (121723) [Unconjugated]

Recombinant Rat CNTFR alpha Protein

Recombinant Human CNTFR alpha Protein

Recombinant Human CLC/CNTFR alpha Chimera Protein

CNTFR Antibody

CNTFR Antibody - BSA Free

CNTFR Antibody - BSA Free

CNTFR Antibody (MM0190-10L29) - Azide and BSA Free

CNTFR Antibody - BSA Free

CNTFR Antibody - BSA Free