Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-45283SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1584-SP | |||
| Carrier Free | IC1584U-100UG | |||
| Carrier Free | IC1584T-100UG | |||
| Carrier Free | IC1584S-100UG | |||
| Carrier Free | IC1584R-100UG | |||
| Carrier Free | IC1584N-100UG | |||
| Carrier Free | IC1584G-100UG | |||
| Carrier Free | IC1584V-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB21441-SP | |||
| Carrier Free | IC21441V-100UG | |||
| Carrier Free | IC21441U-100UG | |||
| Carrier Free | IC21441T-100UG | |||
| Carrier Free | IC21441S-100UG | |||
| Carrier Free | IC21441R-100UG | |||
| Carrier Free | IC21441N-100UG | |||
| Carrier Free | IC21441G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4376-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74571SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21678 | |||
| DyLight 488 | NBP1-77701 | |||
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0019.jpg "Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here. This image was submitted via customer Review.")
![Immunohistochemistry: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Immunohistochemistry-NB100-1687-img0015.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Affinity purified phosphospecific antibody to monophosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19 was used at a 1:1000 dilution to detect myosin light chain by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated or insulin or CLA treated according as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gta- Rabbit IgG (611-103-122) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0014.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0016.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody: Myosin pS19/pS20 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Regulatory Light Chain Phospho. Other band(s): None.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0017.jpg)
![Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.](http://images.novusbio.com/fullsize/MRCL3-[p-Ser19--p-Ser20]-Antibody-Western-Blot-NB100-1687-img0018.jpg)
![Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SNX27 on mouse brain extracts.](http://images.novusbio.com/fullsize/SNX27-Antibody-Western-Blot-NBP1-45283-img0009.jpg "Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SNX27 on mouse brain extracts.")
![Immunocytochemistry/Immunofluorescence: SNX27 Antibody [NBP1-45283] - Detection of SNX27 (Green) in Hela cells using NBP1-45283. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/SNX27-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-45283-img0006.jpg)
![Immunohistochemistry: SNX27 Antibody [NBP1-45283] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SNX27-Antibody-Immunohistochemistry-NBP1-45283-img0005.jpg)
![Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SXN27 antibody in A. 293T + tagged SNX27 protein and B. 293T empty vector.](http://images.novusbio.com/fullsize/SNX27-Antibody-Western-Blot-NBP1-45283-img0008.jpg)
![Simple Western: SNX27 Antibody [NBP1-45283] - Simple Western lane view shows a specific band for SNX27 in 0.5 mg/ml of Human Cerebellum (left) and Mouse Cerebellum (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SNX27-Antibody-Simple-Western-NBP1-45283-img0010.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Western-Blot-NB110-74571-img0004.jpg "Western Blot: Striatin Antibody [NB110-74571] - Immunoblot of PP2A immunoprecipitate with NB110-74571.")
![Immunocytochemistry/Immunofluorescence: Striatin Antibody [NB110-74571] - Immunofluorescent staining of NIH3T3 cells ± blocking peptide using NB110-74571.](http://images.novusbio.com/fullsize/Striatin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-74571-img0003.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0011.jpg "Western Blot: Staufen Antibody [NBP1-33202] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ expanded) FBs were used as additional controls. Four normal and five SCA2 FBs, and two normal and three SCA2 LBCs were used.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194296/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Staufen Antibody [NBP1-33202] - Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33202-img0008.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Sample (30 ug of whole cell lysate) A: Neuro2A diluted at 1:1000](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0005.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Staufen antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Staufen-Antibody-Western-Blot-NBP1-33202-img0010.jpg)
![Immunoprecipitation: Staufen Antibody [NBP1-33202] - Staufen antibody staining 30 ug whole cell lysate of Lane A: 293 (input), Lane B: control rabbit IgG-IP, Lane C: Stau1-IP, Lane D: Post-IP lysate from control rabbit IgG-IP, Lane E:Post-IP lysate from Stau1-IP; diluted at 1:3000.](http://images.novusbio.com/fullsize/Staufen-Antibody-Immunoprecipitation-NBP1-33202-img0006.jpg)
![Western Blot: Staufen Antibody [NBP1-33202] - Silencing of STAU1 mitigates SCA2 phenotypes. aStau1 haploinsufficiency improves abnormal motor behavior of ATXN2Q127 mice as determined by rotarod behavior at 8, 12, 16, & 20 weeks of age. ATXN2Q127;Stau1+/- mice (green) have improved rotarod performance compared with ATXN2Q127 littermates (red) starting at 12 weeks of age. Note that Stau1 haploinsufficiency (orange) by itself does not alter motor function; n = 9–15 mice per group. Values shown are mean ± SE. Significance was determined using generalized estimating equations (GEE). NS, nonsignificant, *P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P  4 pixels per cell positive for both ATXN2 & STAU1 are shown. One-hundred normal & 96 SCA2-FBs were used for analyses. Data are mean ± SD, **P Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Staufen stained by Staufen antibody (NBP1-33202) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33202_rabbit-polyclonal-staufen-antibody-immunocytochemistry-immunofluorescence-26112025185041.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0011.jpg "Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A431 cells. Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins. A massive increase in AKT-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin. Panel A shows STED data (AKT-pS473, red channel) collected simultaneously with confocal signal (alpha-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT pS473 at the cell periphery.")
![Flow Cytometry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the DyLight 488 conjugate of AKT1 phospho Ser473 antibody. Image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Flow-Cytometry-NBP1-69923-img0021.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). A: Lane 1) PDGF stimulated NIH 3T3 cells [10ul]. Lane 2) NIH 3T3 cells [10ul]. Lane 3) Hela whole cell lysate [10ul] (weak signal). B: Lane 4) GST negative control protein [100ng]. Lane 5) GST negative control protein [25ng]. Lane 6) AKT 1 recombinant protein [100ng]. Lane 7) AKT 1 recombinant protein [25ng].Block: 5% BSA overnight at 4C.Primary antibody: Monoclonal anti-AKT antibody used at 1:1000 for overnight at 4C.Secondary antibody: HRP Conjugated goat anti-mouse 1:25K for 45 min at RT.Detection: TMB for 20 minutes, rinsed with deionized water, dried and scanned on conventional flatbed scanner.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0045.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - High resolution STED immunofluorescence nanoscopy of AKT11 [p Ser473] antibody (17F6.B11). Tissue: A431 cells. The merge images (A) and at high magnification (B) show phosphorylated AKT1 colocalized with the distal microtubules. Fixation: 4% paraformaldehyde for 5 min and after washes blocked with 10% NGS/0.2% Triton X-100 for 30 min.Antigen retrieval: serum deprivation for 12 h.Primary antibody: AKT1 pS473 antibody at 10 ug/mL and alpha-tubulin (cyan) at 1.4 ug/mL for 1 h at RT.Secondary antibody: Atto 647N anti-Mouse IgG (ATTO TEC GmbH), and DyLight(TM)488 anti-Rabbit IgG were used at 1.0 ug/mL for 1h at RT for indirect detection.Localization: AKT1 pS473 is in the cytoplasm and also organized at the periphery of the cell.Staining: AKT1 pS473 as red signal with bis-benzimide (blue) nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0040.jpg)
![Immunohistochemistry: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunohistochemistry of AKT11 [p Ser473] antibody (17F6.B11). Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: AKT1 pS473 antibody at 20 ug/mL for 1 h at RT.Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT.Localization: AKT1 pS473 is nuclear and occasionally cytoplasmic.Staining: AKT1 pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-NBP1-69923-img0046.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunoblotting analysis. Representative images of the immunoblot analysis for (A) Akt, (B) p53 (C) AMPK, and (D) LC3 at 24 h of incubation with or without 20 mM dG are shown. beta-actin was used as loading control. Densitometry analysis was performed and the Akt-P/Akt ratio was calculated. The results are the mean +/- SEM of three independent experiments. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/7/2115) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0037.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: untreated NIH/3T3 cell lysates. Lane 2: detects phosphorylated AKT1 (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 ug per lane.Primary antibody: AKT1 pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4 C.Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 1 h at 4 C with FemtoMax(TM) enhanced chemiluminescent reagent.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0042.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT11 [p Ser473] antibody (17F6.B11). Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane.Primary antibody: AKT1pS473 antibody at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 pS473 Monoclonal Antibody at 1:10,000 for 45 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C.Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0043.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Western Blot of AKT1 [p Ser473] antibody (17F6.B11). Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated AKT11, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated AKT11 (red band). Load: 10 ug per lane.Primary antibody: rabbit anti-AKT1 (pan) and mouse anti-AKT1 pS473 specific antibodies at 1:400 for overnight at 4C.Secondary antibody: DyLight(TM) 549 conjugated anti-rabbit IgG (green) and DyLight(TM) 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Western-Blot-NBP1-69923-img0044.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of DyLight 488 conjugate of NBP1-69923. This image shows anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0035.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923]](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Immunofluorescence Microscopy of AKT11 [p Ser473] antibody (17F6.B11) using STED nanoscopy to evaluate AKT1 activation and migration. Tissue: A431 cells. Antigen retrieval: Panel A: serum starved, unstimulated cells. Panel B: serum starved, EGF stimulated for 15 mins.A massive increase in AKT1-pS473 activation, as measured by intensity signal, peaked at 15 minutes and was associated with depolymerized tubulin.Staining: Panel A shows STED data (AKT1-pS473, red channel) collected simultaneously with confocal signal (a-tubulin, green channel). Upon stimulation of cells with EGF, a rapid activation of AKT1 is observed (Panel B) along with a coincident change in the tubulin organization (yellow signal), as well as an extensive cell shape-change (cell membrane folding) and accumulation of AKT1pS473 at the cell periphery.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunocytochemistry-Immunofluorescence-NBP1-69923-img0039.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of Biotin conjugate of NBP1-69923. 20 ug/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear count](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0034.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis of FFPE prostate tissue using AKT1 phospho Ser473 antibody (left panel). Negative control (right panel). Antigen retrieval with heat and pressure in citrate buffer pH 6.2. AKT1 phospho Ser473 antibody at 20 ug/mL. Secondary antibody Streptavidin-HRP at 10 ug/mL. Hematoxylin nuclear counterstain (purple). Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-17F6-B11-Immunohistochemistry-Paraffin-NBP1-69923-img0036.jpg)
![Western Blot of Mouse Anti-AKTpS473 antibody. Lane 1: A431 cells. Lane 2: A431 cells stimulated for 15 min with EGF. Load: 35 ug per lane. Primary antibody: AKTpS473 antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight(TM)649 Conjugated Anti-AKT1 [p Ser473] Antibody (17F6.B11) at 1:10,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting overnight at 4C. Predicted/Observed size: 56kDa. Other band(s): none.](http://images.novusbio.com/fullsize/nbp1-69923_mouse-monoclonal-akt1-p-ser473-antibody-17f6-b11-255202313136.jpg)
![Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB600-1043-img0009.jpg "Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.")
![Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043] - Osteopontin/OPN/OPN antibody was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin/OPN is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin/OPN is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-NB600-1043-img0010.jpg)
![Osteopontin/OPN Antibody [NB600-1043]](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-N-A-NB600-1043-img0008.jpg)
![Chromatin Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of OCT7 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.](http://images.novusbio.com/fullsize/OCT7-Antibody-Chromatin-Immunoprecipitation-NBP2-21585-img0004.jpg "Chromatin Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of OCT7 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.")
![Immunohistochemistry-Paraffin: POU3F2/OCT7 Antibody [NBP2-21585] - TOV-21G xenograft, using Brn2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/OCT7-Antibody-Immunohistochemistry-Paraffin-NBP2-21585-img0002.jpg)
![Immunoprecipitation: POU3F2/OCT7 Antibody [NBP2-21585] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of OCT7 protein by 2 ug of Brn2 antibody 10% SDS-PAGE The immunoprecipitated OCT7 protein was detected by OCT7. antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/OCT7-Antibody-Immunoprecipitation-NBP2-21585-img0003.jpg)
![Western Blot: POU3F2/OCT7 Antibody [NBP2-21585] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with POU3F2/OCT7 antibody (NBP2-21585) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-21585_rabbit-polyclonal-pou3f2-oct7-antibody-91020242041295.jpg)
![Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (NBP2-21585) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-21585_rabbit-polyclonal-pou3f2-oct7-antibody-immunohistochemistry-frozen-2611202518204713.jpg)
![Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Brn2 protein stained by Brn2 antibody (NBP2-21585) diluted at 1:1000.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-21585_rabbit-polyclonal-pou3f2-oct7-antibody-immunocytochemistry-immunofluorescence-2611202518484821.jpg)
