Flow Cytometry is a laser based biological assay used for cell detection, counting and sorting of specific biomarkers. Cells are first suspended in solution and fluorescently labeled antibodies are applied. Then the cells are passed single-file through a series of lasers which analyze size, shape and the fluorescent characteristics of each cell. Results can then be electronically gated to filter the data to a specific cell type and whether or not they contain bound antibodies. This form of assay is particularly useful for analyzing blood samples.
Novus offers a range of support options for flow cytometry when you buy a product to help you with your flow cytometry experiment including over 1,000 Flow images using our pre-conjugated antibodies:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71834 | |||
![Western Blot: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.](http://images.novusbio.com/fullsize/IL1F6-Antibody-1A4-Western-Blot-NBP2-02758-img0002.jpg "Western Blot: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1F6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1F6.")
![Immunocytochemistry/Immunofluorescence: IL1F6 Antibody (1A4) [NBP2-02758] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL1F6.](http://images.novusbio.com/fullsize/IL1F6-Antibody-1A4-Immunocytochemistry-Immunofluorescence-NBP2-02758-img0003.jpg)
![Flow Cytometry: IL1F6 Antibody (1A4) [NBP2-02758] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IL1F6 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IL1F6-Antibody-1A4-Flow-Cytometry-NBP2-02758-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FBXO44 Antibody [NBP1-51915] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/FBXO44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51915-img0002.jpg "Immunocytochemistry/Immunofluorescence: FBXO44 Antibody [NBP1-51915] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: FBXO44 Antibody [NBP1-51915] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/FBXO44-Antibody-Flow-Cytometry-NBP1-51915-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FBXO44 Antibody [NBP1-51915] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/FBXO44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51915-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DISP1 Antibody [NB100-1408] - (2.5ug/ml) staining of U87. Detected by mmunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.](http://images.novusbio.com/fullsize/DISP1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1408-img0003.jpg "Immunocytochemistry/Immunofluorescence: DISP1 Antibody [NB100-1408] - (2.5ug/ml) staining of U87. Detected by mmunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.")
![Flow Cytometry: DISP1 Antibody [NB100-1408] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DISP1-Antibody-Flow-Cytometry-NB100-1408-img0004.jpg)
![Flow Cytometry: PATJ Antibody [NB100-1354] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PATJ-Antibody-Flow-Cytometry-NB100-1354-img0004.jpg "Flow Cytometry: PATJ Antibody [NB100-1354] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Immunofluorescence: PATJ Antibody [NB100-1354] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing junctional staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PATJ-Antibody-Immunofluorescence-NB100-1354-img0003.jpg)
![Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Western-Blot-NB100-93365-img0002.jpg "Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ABCB5-Antibody-Flow-Cytometry-NB100-93365-img0001.jpg)
![Western Blot: P2X4 Antibody [NBP1-00141] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/P2X4-Antibody-Western-Blot-NBP1-00141-img0001.jpg "Western Blot: P2X4 Antibody [NBP1-00141] - Staining of Human Kidney lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: P2X4 Antibody [NBP1-00141] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/P2X4-Antibody-Flow-Cytometry-NBP1-00141-img0002.jpg)
![Immunohistochemistry: HOXD10 Antibody [NBP1-28476] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HOXD10-Antibody-Immunohistochemistry-NBP1-28476-img0003.jpg "Immunohistochemistry: HOXD10 Antibody [NBP1-28476] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: HOXD10 Antibody [NBP1-28476] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/HOXD10-Antibody-Flow-Cytometry-NBP1-28476-img0002.jpg)
![Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.](http://images.novusbio.com/fullsize/NFIL3-Antibody-Western-Blot-NB100-842-img0003.jpg "Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.")
![Flow Cytometry: NFIL3 Antibody [NB100-842] - analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized](http://images.novusbio.com/fullsize/NFIL3-Antibody-Flow-Cytometry-NB100-842-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP1-00171] - Analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00171-img0001.jpg "Immunocytochemistry/Immunofluorescence: CLEC16A Antibody [NBP1-00171] - Analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong nuclear and weak cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: CLEC16A Antibody [NBP1-00171] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CLEC16A-Antibody-Flow-Cytometry-NBP1-00171-img0002.jpg)
![Western Blot: NKG2D/CD314 Antibody [NB100-1891] - Staining of MOLT-4 cell lysate (A) + peptide (B) with antibody at 0.5 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-Western-Blot-NB100-1891-img0001.jpg "Western Blot: NKG2D/CD314 Antibody [NB100-1891] - Staining of MOLT-4 cell lysate (A) + peptide (B) with antibody at 0.5 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: NKG2D/CD314 Antibody [NB100-1891] - Flow cytometric analysis of human peripheral blood monocytes. Primary incubation 30 minutes (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).](http://images.novusbio.com/fullsize/NKG2D-CD314-Antibody-Flow-Cytometry-NB100-1891-img0002.jpg)
![Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) PE.](http://images.novusbio.com/fullsize/CD229-SLAMF3-Lymphocyte-Antigen-9-Antibody-HLy9-25-Flow-Cytometry-NBP1-50070-img0002.jpg "Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) PE.")
![Flow Cytometry: CD229/SLAMF3/Lymphocyte Antigen 9 Antibody (HLy9.25) [NBP1-50070] - Surface staining of human peripheral blood cells with anti-human CD229 (HLy9.25) APC.](http://images.novusbio.com/fullsize/CD229-SLAMF3-Lymphocyte-Antigen-9-Antibody-HLy9-25-Flow-Cytometry-NBP1-50070-img0001.jpg)
![Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SCD-1-Antibody-Immunohistochemistry-Paraffin-NB100-2476-img0005.jpg "Immunohistochemistry-Paraffin: SCD-1 Antibody [NB100-2476] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: SCD Antibody [NB100-2476] - staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 (CYT-450C) in red.](http://images.novusbio.com/fullsize/SCD-Antibody-Flow-Cytometry-NB100-2476-img0004.jpg)
![Immunohistochemistry-Paraffin: SCD Antibody [NB100-2476] - (5ug/ml) staining Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SCD-Antibody-Immunohistochemistry-Paraffin-NB100-2476-img0002.jpg)